Q46FY8 (ILVC_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Mbar_A0220

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000226217
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q46FY8 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 5B77DB29A7BD7356	FASTA	335	36,994
	10 20 30 40 50 60 MAKIIYDNET TFDALKDKTI AIMGYGSQGH AHARNLHESG LNVIVGLRKS SSSWAKAEND 70 80 90 100 110 120 GLKVMTVAEA AKAADVIMIL LPDENQASVY YSEIAPNLEA GNALAFAHGF NIHYNQIVPP 130 140 150 160 170 180 KDVDVFMAAP KGPGHIVRRT YTEGIGVPAL IAVYQDATGN AREIALSYVK GIGATRAGVY 190 200 210 220 230 240 ETTFREETET DLFGEQVDLC GGLSSLIKTA FETLVEAGYQ PEMAYFETCH EVKLIVDLIY 250 260 270 280 290 300 EGGLERMWHS VSNTAEYGGM TVGPRIINDE SREAMREALK RIQNGEFAKE FVLEGMVNHP 310 320 330 VLKAMERQEK EHQLEVVGKQ IRANIPWLNR EIDDD

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69204.1.
RefSeq	YP_303784.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46FY8.
SMR	Q46FY8. Positions 3-328.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46FY8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3626000.
GenomeReviews	Gene locus Mbar_A0220 in contig CP000099_GR.
KEGG	mba:Mbar_A0220.
NMPDR	fig|269797.3.peg.304.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04335.
HOGENOM	HBG286525.
OMA	EYANGRG.
PhylomeDB	Q46FY8.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0220-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_METBF

Accession

Primary (citable) accession number: Q46FY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program