ID   MER_METBF               Reviewed;         328 AA.
AC   Q46FV4;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 27.
DE   RecName: Full=5,10-methylenetetrahydromethanopterin reductase;
DE            EC=1.5.99.11;
DE   AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase;
DE   AltName: Full=Methylene-H(4)MPT reductase;
GN   Name=mer; OrderedLocusNames=Mbar_A0254;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16980466; DOI=10.1128/JB.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P.,
RA   Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R.,
RA   Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT
CC       to methyl-H(4)MPT (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5-methyltetrahydromethanopterin + coenzyme
CC       F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme
CC       F420.
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from carbone
CC       dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 1/2.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the mer family.
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DR   EMBL; CP000099; AAZ69238.1; -; Genomic_DNA.
DR   RefSeq; YP_303818.1; -.
DR   PDB; 1Z69; X-ray; 2.61 A; A/B/C/D=1-327.
DR   PDBsum; 1Z69; -.
DR   SMR; Q46FV4; 1-327.
DR   GeneID; 3624867; -.
DR   GenomeReviews; CP000099_GR; Mbar_A0254.
DR   KEGG; mba:Mbar_A0254; -.
DR   NMPDR; fig|269797.3.peg.370; -.
DR   HOGENOM; Q46FV4; -.
DR   OMA; Q46FV4; LNEISDG.
DR   BioCyc; MBAR269797:MBAR_A0254-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetr...; IEA:HAMAP.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01091; -; 1.
DR   InterPro; IPR011251; Luciferase-like.
DR   InterPro; IPR016048; Luciferase-like_sub.
DR   InterPro; IPR019946; MeH4methanopterin_reductase.
DR   Gene3D; G3DSA:3.20.20.30; Luciferase_like; 1.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   TIGRFAMs; TIGR03555; F420_mer; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Cytoplasm; Methanogenesis;
KW   One-carbon metabolism; Oxidoreductase.
FT   CHAIN         1    328       5,10-methylenetetrahydromethanopterin
FT                                reductase.
FT                                /FTId=PRO_1000064889.
SQ   SEQUENCE   328 AA;  34592 MW;  AC4BD2A64B93ADC7 CRC64;
     MKFGIEFVPS DPALKIAYYA KLSEQQGFDH VWITDHYNNR DVYSTLTVLA LNTNSIKIGP
     GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWKKP LATTKEAIQA
     IRDFISGKKV SMDGEMIKFA GAKLAFKAGN IPIYMGAQGP KMLELAGEIA DGVLINASHP
     KDFEVAVEQI KKGAEKAGRD PSEVDVTAYA CFSIDKDPVK AVNAAKVVVA FIVAGSPDLV
     LERHGIPVEA KSQIGAAIAK GDFGALMGGL VTPQMIEAFS ICGTPDDCMK RIKDLEAIGV
     TQIVAGSPIG PAKEKAIKLI GKEIIAKM
//