Reviewed, UniProtKB/Swiss-Prot Q46FV4 (MER_METBF)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydromethanopterin reductase
    EC=1.5.99.11
Alternative name(s):
    Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
    Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	Mbar_A0254

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT By similarity.
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 1/2. HAMAP MF_01091
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091		PRO_1000064889

Sequences

	Sequence		Length	Mass (Da)
	Q46FV4-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: AC4BD2A64B93ADC7	FASTA	328	34,592
	10 20 30 40 50 60 MKFGIEFVPS DPALKIAYYA KLSEQQGFDH VWITDHYNNR DVYSTLTVLA LNTNSIKIGP 70 80 90 100 110 120 GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWKKP LATTKEAIQA 130 140 150 160 170 180 IRDFISGKKV SMDGEMIKFA GAKLAFKAGN IPIYMGAQGP KMLELAGEIA DGVLINASHP 190 200 210 220 230 240 KDFEVAVEQI KKGAEKAGRD PSEVDVTAYA CFSIDKDPVK AVNAAKVVVA FIVAGSPDLV 250 260 270 280 290 300 LERHGIPVEA KSQIGAAIAK GDFGALMGGL VTPQMIEAFS ICGTPDDCMK RIKDLEAIGV 310 320 TQIVAGSPIG PAKEKAIKLI GKEIIAKM

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

CP000099 Genomic DNA. Translation: AAZ69238.1.

RefSeq

YP_303818.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z69	X-ray	2.61	A/B/C/D	1-327	[»]

SMR

Q46FV4. Positions 1-327.

ModBase

Search...

Genome annotation databases

GeneID

3624867.

GenomeReviews

Gene locus Mbar_A0254 in contig CP000099_GR.

KEGG

mba:Mbar_A0254.

NMPDR

fig|269797.3.peg.370.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q46FV4.

Enzyme and pathway databases

BioCyc

MBAR269797:MBAR_A0254-MON.

Family and domain databases

HAMAP

MF_01091.
[Tree]

InterPro

IPR011251. Luciferase-like_bac.
IPR016048. Luciferase_mOase.
[Graphical view]

Gene3D

G3DSA:3.20.20.30. Luciferase_like. 1 hit.

Pfam

PF00296. Bac_luciferase. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MER_METBF

Accession

Primary (citable) accession number: Q46FV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 13, 2005
Last modified:	November 4, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)