Q46FV4 (MER_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydromethanopterin reductase
EC=1.5.99.11

Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	Mbar_A0254

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT By similarity. HAMAP MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP MF_01091
Subcellular location	Cytoplasm By similarity HAMAP MF_01091.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091		PRO_1000064889

Sequences

	Sequence		Length	Mass (Da)
	Q46FV4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: AC4BD2A64B93ADC7	FASTA	328	34,592
	10 20 30 40 50 60 MKFGIEFVPS DPALKIAYYA KLSEQQGFDH VWITDHYNNR DVYSTLTVLA LNTNSIKIGP 70 80 90 100 110 120 GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWKKP LATTKEAIQA 130 140 150 160 170 180 IRDFISGKKV SMDGEMIKFA GAKLAFKAGN IPIYMGAQGP KMLELAGEIA DGVLINASHP 190 200 210 220 230 240 KDFEVAVEQI KKGAEKAGRD PSEVDVTAYA CFSIDKDPVK AVNAAKVVVA FIVAGSPDLV 250 260 270 280 290 300 LERHGIPVEA KSQIGAAIAK GDFGALMGGL VTPQMIEAFS ICGTPDDCMK RIKDLEAIGV 310 320 TQIVAGSPIG PAKEKAIKLI GKEIIAKM

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000099 Genomic DNA. Translation: AAZ69238.1.

RefSeq

YP_303818.1. NC_007355.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z69	X-ray	2.61	A/B/C/D	1-327	[»]

ProteinModelPortal

Q46FV4.

SMR

Q46FV4. Positions 1-327.

ModBase

Search...

Protein-protein interaction databases

STRING

Q46FV4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3624867.

GenomeReviews

Gene locus Mbar_A0254 in contig CP000099_GR.

KEGG

mba:Mbar_A0254.

NMPDR

fig|269797.3.peg.370.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

arNOG04928.

HOGENOM

HBG497169.

OMA

GIEFVPG.

ProtClustDB

PRK02271.

Enzyme and pathway databases

BioCyc

MBAR269797:MBAR_A0254-MONOMER.

Family and domain databases

HAMAP

MF_01091. F420_mer.
[Tree]

InterPro

IPR011251. Luciferase-like_dom.
IPR019946. MeH4methanopterin_reductase.
[Graphical view]

Gene3D

G3DSA:3.20.20.30. Luciferase_like. 1 hit.

KO

K00320.

Pfam

PF00296. Bac_luciferase. 1 hit.
[Graphical view]

SUPFAM

SSF51679. Luciferase_like. 1 hit.

TIGRFAMs

TIGR03555. F420_mer. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MER_METBF

Accession

Primary (citable) accession number: Q46FV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program