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Q46FS9 (KAE1B_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. tRNA N6-adenosine threonylcarbamoyltransferase
    EC=2.3.1.-
    Alternative name(s):
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
  2. Serine/threonine-protein kinase Bud32
    EC=2.7.11.1
Gene names
Ordered Locus Names:Mbar_A0279
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length545 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01447

Subunit structure

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / TsaD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 545545Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000303655

Regions

Domain340 – 545206Protein kinase
Nucleotide binding353 – 3619ATP By similarity
Region1 – 329329Kae1 HAMAP-Rule MF_01447
Region134 – 1385Threonylcarbamoyl-AMP binding By similarity

Sites

Active site4621Proton acceptor; for kinase activity By similarity
Metal binding1131Iron By similarity
Metal binding1171Iron By similarity
Metal binding1341Iron By similarity
Metal binding2901Iron By similarity
Binding site1661Threonylcarbamoyl-AMP By similarity
Binding site1791Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding site1831Threonylcarbamoyl-AMP By similarity
Binding site2621Threonylcarbamoyl-AMP By similarity
Binding site3751ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46FS9 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: B1DA7E54DB69929B

FASTA54560,247
        10         20         30         40         50         60 
MKNTFILGIE GTAWNLSAAI VTETEIIAEV TETYKPTAGG IHPREAAQHH AKYAASVIKR 

        70         80         90        100        110        120 
LLAEAKEKGV KPSDIDGIAF SQGPGLGPCL RTVATAARML SISLGIPLIG VNHCIAHIEI 

       130        140        150        160        170        180 
GIWRTPAMDP VVLYVSGANS QVISYMGGRY RVFGETLDIG LGNALDKFAR GANLPHPGGP 

       190        200        210        220        230        240 
KIEAYAKNAT KYIHLPYVIK GMDLSFSGLS TAASEALKKA PLEDVCYSYQ ETAFAMVVEV 

       250        260        270        280        290        300 
AERALAHTGK KEVLLAGGVG ANTRLREMLN DMCEARGAKF YVPEKRFMGD NGTMIAYTGL 

       310        320        330        340        350        360 
LMYKSGNTLS LEDSRVNPSY RTDDVKVTWI QEEKMKRVPE ISPGTSLKLG ELLDNGAEAI 

       370        380        390        400        410        420 
VYLEEGPEGK KILVKERVPK AYRYKEIDER IRTERNRTEA RLMSESRRHG VSTPIIYDVE 

       430        440        450        460        470        480 
DFKLKMQYID GVPIKYLVTP ELSEKVGELV GKLHSAGIVH GDLTTSNILL AGERLYLLDF 

       490        500        510        520        530        540 
GLAYYDKGLE ARGVDVHVLF QTFESTHRNH EALIEAFKKG YRRTFIDSED VLTRVEEIKK 


RARYA 

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ69263.1.
RefSeqYP_303843.1. NC_007355.1.

3D structure databases

ProteinModelPortalQ46FS9.
SMRQ46FS9. Positions 5-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269797.Mbar_A0279.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ69263; AAZ69263; Mbar_A0279.
GeneID3624399.
KEGGmba:Mbar_A0279.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
HOGENOMHOG000109569.
KOK15904.
OMARDNAGMI.
ProtClustDBPRK09605.

Enzyme and pathway databases

BioCycMBAR269797:GHUW-287-MONOMER.

Family and domain databases

HAMAPMF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERPTHR11735:SF6. PTHR11735:SF6. 1 hit.
PfamPF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. SSF56112. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_METBF
AccessionPrimary (citable) accession number: Q46FS9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families