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Q46FS9

- KAE1B_METBF

UniProt

Q46FS9 - KAE1B_METBF

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Protein
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Gene
Mbar_A0279
Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Iron By similarity
Metal bindingi117 – 1171Iron By similarity
Metal bindingi134 – 1341Iron By similarity
Binding sitei166 – 1661Threonylcarbamoyl-AMP By similarity
Binding sitei179 – 1791Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding sitei183 – 1831Threonylcarbamoyl-AMP By similarity
Binding sitei262 – 2621Threonylcarbamoyl-AMP By similarity
Metal bindingi290 – 2901Iron By similarity
Binding sitei375 – 3751ATP By similarity
Active sitei462 – 4621Proton acceptor; for kinase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi353 – 3619ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
  5. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  6. protein tyrosine kinase activity Source: InterPro
  7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  8. zinc ion binding Source: InterPro

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-287-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
Serine/threonine-protein kinase Bud32 (EC:2.7.11.1)
Gene namesi
Ordered Locus Names:Mbar_A0279
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 545545Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
PRO_0000303655Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Protein-protein interaction databases

STRINGi269797.Mbar_A0279.

Structurei

3D structure databases

ProteinModelPortaliQ46FS9.
SMRiQ46FS9. Positions 5-330.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini340 – 545206Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 329329Kae1UniRule annotation
Add
BLAST
Regioni134 – 1385Threonylcarbamoyl-AMP binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109569.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46FS9-1 [UniParc]FASTAAdd to Basket

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MKNTFILGIE GTAWNLSAAI VTETEIIAEV TETYKPTAGG IHPREAAQHH    50
AKYAASVIKR LLAEAKEKGV KPSDIDGIAF SQGPGLGPCL RTVATAARML 100
SISLGIPLIG VNHCIAHIEI GIWRTPAMDP VVLYVSGANS QVISYMGGRY 150
RVFGETLDIG LGNALDKFAR GANLPHPGGP KIEAYAKNAT KYIHLPYVIK 200
GMDLSFSGLS TAASEALKKA PLEDVCYSYQ ETAFAMVVEV AERALAHTGK 250
KEVLLAGGVG ANTRLREMLN DMCEARGAKF YVPEKRFMGD NGTMIAYTGL 300
LMYKSGNTLS LEDSRVNPSY RTDDVKVTWI QEEKMKRVPE ISPGTSLKLG 350
ELLDNGAEAI VYLEEGPEGK KILVKERVPK AYRYKEIDER IRTERNRTEA 400
RLMSESRRHG VSTPIIYDVE DFKLKMQYID GVPIKYLVTP ELSEKVGELV 450
GKLHSAGIVH GDLTTSNILL AGERLYLLDF GLAYYDKGLE ARGVDVHVLF 500
QTFESTHRNH EALIEAFKKG YRRTFIDSED VLTRVEEIKK RARYA 545
Length:545
Mass (Da):60,247
Last modified:September 13, 2005 - v1
Checksum:iB1DA7E54DB69929B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ69263.1.
RefSeqiYP_303843.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ69263; AAZ69263; Mbar_A0279.
GeneIDi3624399.
KEGGimba:Mbar_A0279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ69263.1 .
RefSeqi YP_303843.1. NC_007355.1.

3D structure databases

ProteinModelPortali Q46FS9.
SMRi Q46FS9. Positions 5-330.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269797.Mbar_A0279.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ69263 ; AAZ69263 ; Mbar_A0279 .
GeneIDi 3624399.
KEGGi mba:Mbar_A0279.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109569.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci MBAR269797:GHUW-287-MONOMER.

Family and domain databases

HAMAPi MF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiKAE1B_METBF
AccessioniPrimary (citable) accession number: Q46FS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 13, 2005
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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