Q46FR7 (G1PDH_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Ordered Locus Names:	Mbar_A0291

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497_A
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497_A
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497_A
Subcellular location	Cytoplasm Potential HAMAP MF_00497_A.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_A		PRO_1000050602
Regions
	Nucleotide binding	103 – 107	5	NAD By similarity
	Nucleotide binding	125 – 128	4	NAD By similarity
Sites
	Metal binding	177	1	Zinc; catalytic By similarity
	Metal binding	257	1	Zinc; catalytic By similarity
	Metal binding	273	1	Zinc; catalytic By similarity
	Binding site	130	1	Substrate By similarity
	Binding site	134	1	NAD By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	261	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46FR7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 3343A27525A82D49	FASTA	356	37,990
	10 20 30 40 50 60 MKLTINKNSA KWMQLPRDVL VGHGVLEEIG DVCRDLKMKG NALIVTGSTT NNIAGKRVSN 70 80 90 100 110 120 LLESAGCSAE MVLTCKATKE EVEKVMEKAL EVETNFLIGV GSGRSIDLAK LASTRLELPF 130 140 150 160 170 180 ISVPTAASHD GIASSRASIV DNGRSTSAQA QAPIAVIADT EIISAAPFRF LAAGCGDIIS 190 200 210 220 230 240 NYTAVLDWEL ASRLRNEYFG AYAAALSRMA ARVIIECADS IKPEHETSAR LVVKALVSNG 250 260 270 280 290 300 VAMSIAGSSR PASGSEHMFS HALDKIAPKP ALHGEQCGVG TIMMMYLHGG NWQEIREALK 310 320 330 340 350 KIGAPVTAEE LGIEDRYIIE ALLHAHSIRP ERYTILGSGL NPSAAEKVAR ITKVIN

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69275.1.
RefSeq	YP_303855.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46FR7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46FR7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3624411.
GenomeReviews	Gene locus Mbar_A0291 in contig CP000099_GR.
KEGG	mba:Mbar_A0291.
NMPDR	fig|269797.3.peg.438.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04488.
HOGENOM	HBG672951.
OMA	CGVGTIM.
PhylomeDB	Q46FR7.
ProtClustDB	PRK00843.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0291-MONOMER.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR023002. G1P_dehydrogenase_arc. IPR016205. Glycerol_DH. [Graphical view]
KO	K00096.
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METBF

Accession

Primary (citable) accession number: Q46FR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program