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Q46FK9

- ENDA_METBF

UniProt

Q46FK9 - ENDA_METBF

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei286 – 2861UniRule annotation
    Active sitei297 – 2971UniRule annotation
    Active sitei328 – 3281UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-357-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:Mbar_A0349
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350tRNA-splicing endonucleasePRO_0000309819Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi269797.Mbar_A0349.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46FK9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000226561.
    KOiK01170.
    OMAiRGFYLSP.

    Family and domain databases

    Gene3Di3.40.1350.10. 2 hits.
    HAMAPiMF_01834. EndA_long.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsiTIGR00324. endA. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q46FK9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTQLKGDRV LAEKEAVAEF YKTGYFGRPK GEGLELSLVE AAFLLSRGKL    50
    EIELEGTMLD FRYFFEQASL RQPNFELKYI VYKDLKERGY YVQPSAADFR 100
    VYPRGSHPGK SAARIFVHVQ SERQLLPVKL LQDSVISAEN VHKQFILAVV 150
    DEESDLTFYE VKTAVPKGEM TEPYPAIKTD ATFLEDRVIA WDAQASETLY 200
    KRGFYGKMLD SERLQLSLVE SLYLFSRGII TVRDRKGKVF SFDEFIEKAS 250
    EIESFFLRKY SAYKNLRDSG HVVKTGFKFG TNFRVYRKVE SIEKIPHSEY 300
    LVNAIPADFE FRLSVMSGAV RLANSVRKKM LFAVEKDEEI EYLDISRTKM 350
    Length:350
    Mass (Da):40,401
    Last modified:September 13, 2005 - v1
    Checksum:iBC26ABA079BA2549
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ69333.1.
    RefSeqiYP_303913.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ69333; AAZ69333; Mbar_A0349.
    GeneIDi3626593.
    KEGGimba:Mbar_A0349.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ69333.1 .
    RefSeqi YP_303913.1. NC_007355.1.

    3D structure databases

    ProteinModelPortali Q46FK9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A0349.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ69333 ; AAZ69333 ; Mbar_A0349 .
    GeneIDi 3626593.
    KEGGi mba:Mbar_A0349.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000226561.
    KOi K01170.
    OMAi RGFYLSP.

    Enzyme and pathway databases

    BioCyci MBAR269797:GHUW-357-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 2 hits.
    HAMAPi MF_01834. EndA_long.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsi TIGR00324. endA. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.

    Entry informationi

    Entry nameiENDA_METBF
    AccessioniPrimary (citable) accession number: Q46FK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3