Q46F30 (PUR7_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	Mbar_A0531

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137		PRO_1000018729

Sequences

	Sequence		Length	Mass (Da)
	Q46F30 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: BEBF715BEDF30F91	FASTA	237	27,174
	10 20 30 40 50 60 MTREQLYSGK AKTIYKTDDP DTLITEFRNS LTAFNGEKKG EMEKKGYYNA QISKKIFEML 70 80 90 100 110 120 EAEGIKTHFV EMLSDIDMLV KKVEIIKIEV IVRNIAAGSI TKRYPIKEGT VFKTPVLVFD 130 140 150 160 170 180 FKNDEYGDPM LNDDIALALG LATQEELATL RELALKINEL LVPHLDKKGI LLPDFKLEFG 190 200 210 220 230 RRNGEIILAD EISCDTCRFW DKKTGQSMDK DVFRFDKGDI SKAYEEVARR IVPEIFE

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69512.1.
RefSeq	YP_304092.1. NC_007355.1.
3D structure databases
HSSP	HSSP built from PDB template 1KUT based on UniProtKB Q9X0X0.
ProteinModelPortal	Q46F30.
SMR	Q46F30. Positions 1-232.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46F30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3627090.
GenomeReviews	Gene locus Mbar_A0531 in contig CP000099_GR.
KEGG	mba:Mbar_A0531.
NMPDR	fig|269797.3.peg.708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05989.
HOGENOM	HBG306070.
OMA	YKDDALG.
PhylomeDB	Q46F30.
ProtClustDB	PRK09362.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0531-MONOMER.
Family and domain databases
HAMAP	MF_00137. SAICAR_synth. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synth. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K01923.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00081. PurC. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_METBF

Accession

Primary (citable) accession number: Q46F30

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program