Q46F25 (PURL_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Mbar_A0536

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000236675
Regions
	Nucleotide binding	90 – 101	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q46F25 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 35C55000D9D654E3	FASTA	715	76,285
	10 20 30 40 50 60 MLPEEDLKII KKELGREPTL VEQGCFLNLW SEHCSYRSSA PLLKTFTSKG ENVIIGPGDD 70 80 90 100 110 120 AAIIKFDDGY VLAIGMESHN HPSYVDPYNG AATGIGGIVR DIISMGARPI ALMDPLYFGP 130 140 150 160 170 180 LDTPKNMFLF EQIIKGIAGY GNCIGVPVVN GETFFDRRYS GNPLVNVVAV GLCREEEVIT 190 200 210 220 230 240 ARSQKAGNKL VLAGSSTGKD GLGGASFASR DLSESAEAED RPSVQVGDPY TEKLVIEMTL 250 260 270 280 290 300 EAMEKGYIKS CKDLGAAGLG GASAELAAKG GLGAHITADA VTQREPNMNA YEILLAESQE 310 320 330 340 350 360 RMVFEVAPED VDAVLALVAK YDLNGAVVGY LTEKPNYTVE FKGEVVVDIP IDFLTGGAPT 370 380 390 400 410 420 CEKSSVAPIP QVEEGKAPKT PEDLKAAFLK VISSYNIASK EWIYRQYDHE VQLRTVIKPG 430 440 450 460 470 480 EDAGVLKITD KKGLVLSCGC QPRATLLDPY NGGKNVVIEN AMNLAVKGAD GLAIVNCLNF 490 500 510 520 530 540 GNPDRPEIYW QLKNSVLGLG DGARELSIPV VGGNVSLYNE SDEFKTAILP TPSIGIIGKV 550 560 570 580 590 600 NFETPLPSSF FAKSGDAIIL VGETTADMGG SEYYACFEAL NAGKVPSVPK NAPEIIKAVI 610 620 630 640 650 660 EAARSGKLSS AHDLSLGGIA AGLARMCRNS GAKVDLSEVS ELKAEELLFS EAPARALLAT 670 680 690 700 710 SEPEAVLEIL KDVPHMVIGK VEGNSLEVKG KDFEISLSLK EISDAYSSLT RFMMR

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69517.1.
RefSeq	YP_304097.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46F25.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46F25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3627095.
GenomeReviews	Gene locus Mbar_A0536 in contig CP000099_GR.
KEGG	mba:Mbar_A0536.
NMPDR	fig|269797.3.peg.713.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q46F25.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0536-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METBF

Accession

Primary (citable) accession number: Q46F25

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program