Q46EW4 (OGT_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylated-DNA--protein-cysteine methyltransferase
EC=2.1.1.63

Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:	ogt
Ordered Locus Names:	Mbar_A0598

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated By similarity. HAMAP MF_00772
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. HAMAP MF_00772
Subcellular location	Cytoplasm By similarity HAMAP MF_00772.
Miscellaneous	This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles. HAMAP MF_00772
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 158	158	Methylated-DNA--protein-cysteine methyltransferase HAMAP MF_00772		PRO_1000017350
Sites
	Active site	126	1	Nucleophile; methyl group acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46EW4 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9512FB5BCCECD850	FASTA	158	17,887
	10 20 30 40 50 60 MYYDIIESPI GPILLAGNEK GLKHLDFLKG KKRIEIPADW IENKKFFREA ARQLEAYFSG 70 80 90 100 110 120 KLESFDLKLA PEGTDFQKSV WKALCEIPYG ETRTYKEIAV SIGKPRAYRA VGLANNRNPI 130 140 150 AIIIPCHRVI GSDGKLTGYA SGLDIKEFLL KLEENNLR

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69578.1.
RefSeq	YP_304158.1. NC_007355.1.
3D structure databases
HSSP	HSSP built from PDB template 1WRJ based on UniProtKB Q973C7.
ProteinModelPortal	Q46EW4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46EW4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3627658.
GenomeReviews	Gene locus Mbar_A0598 in contig CP000099_GR.
KEGG	mba:Mbar_A0598.
NMPDR	fig|269797.3.peg.781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10742.
HOGENOM	HBG752398.
OMA	RHIDSPL.
PhylomeDB	Q46EW4.
ProtClustDB	PRK00901.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0598-MONOMER.
Family and domain databases
HAMAP	MF_00772. OGT. [Tree]
InterPro	IPR023546. MethylDNA_cys_MeTrfase. IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR008332. MethylG_MeTrfase. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K00567.
Pfam	PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view]
SUPFAM	SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMs	TIGR00589. Ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OGT_METBF

Accession

Primary (citable) accession number: Q46EW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program