Q46EL9 (IF5A_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor 5A

Alternative name(s):
Hypusine-containing protein
eIF-5A

Gene names

Name:	eif5a
Ordered Locus Names:	Mbar_A0695

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina barkeri

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions by promoting the formation of the first peptide bond By similarity. HAMAP-Rule MF_00085
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the eIF-5A family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
PTM	Hypusine
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: InterPro positive regulation of translational elongation Inferred from electronic annotation. Source: InterPro positive regulation of translational termination Inferred from electronic annotation. Source: InterPro translational frameshifting Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ribosome binding Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	Translation initiation factor 5A HAMAP-Rule MF_00085		PRO_0000259437
Amino acid modifications
	Modified residue	35	1	Hypusine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46EL9 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 3A6467D6506BC5F4	FASTA	128	14,238
	10 20 30 40 50 60 MKQQVEVKEL KEGKYVIIDD EACVIKSITK SKPGKHGAAK ARVEAIGLFD GQKRSYIGSV 70 80 90 100 110 120 ANKIYVPIVE RKTAQVISIT GDITQLMDMG DFSTFEIVVP DEYKDKIKEG EEVSYITALG KIKLDIRT

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69673.1.
RefSeq	YP_304253.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46EL9.
ModBase	Search...
Protein-protein interaction databases
STRING	269797.Mbar_A0695.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ69673; AAZ69673; Mbar_A0695.
GeneID	3625762.
KEGG	mba:Mbar_A0695.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0231.
HOGENOM	HOG000106269.
KO	K03263.
OMA	KIWVPII.
ProtClustDB	PRK03999.
Enzyme and pathway databases
BioCyc	MBAR269797:GHUW-768-MONOMER.
Family and domain databases
Gene3D	2.30.30.30. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_00085. eIF-5A.
InterPro	IPR012340. NA-bd_OB-fold. IPR014722. Rib_L2_dom2. IPR019769. Trans_elong_IF5A_hypusine_site. IPR001884. Transl_elong_IF5A. IPR022847. Transl_elong_IF5A_arc. IPR020189. Transl_elong_IF5A_C. IPR008991. Translation_prot_SH3-like. [Graphical view]
PANTHER	PTHR11673. PTHR11673. 1 hit. PTHR11673:SF1. PTHR11673:SF1. 1 hit.
Pfam	PF01287. eIF-5a. 1 hit. [Graphical view]
PIRSF	PIRSF003025. eIF5A. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR00037. eIF_5A. 1 hit.
PROSITE	PS00302. IF5A_HYPUSINE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF5A_METBF

Accession

Primary (citable) accession number: Q46EL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program