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Q46EG2 (DUT_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
dUTP pyrophosphatase
Gene names
Name:dut
Ordered Locus Names:Mbar_A0752
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length171 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00635

Catalytic activity

dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00635

Pathway

Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00635

Sequence similarities

Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.

Ontologies

Keywords
   Biological processNucleotide metabolism
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdUMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

dUTP metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 171171Probable deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00635
PRO_1000061430

Sequences

Sequence LengthMass (Da)Tools
Q46EG2 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 23854036D3A59BD0

FASTA17119,100
        10         20         30         40         50         60 
MTLLSSTELR KLIQATPSLL ENAIDIETQI QPNGLELTLK EVKTIDGSGA VDFDNSERQL 

        70         80         90        100        110        120 
PDGKTLEFGN DGWIHLPKGI YKVLFNEIVN IPMNLAAIAK PRSTLIRCGT TLETAVWDAG 

       130        140        150        160        170 
YRGRSESMLV VYNTEGFRLK KDARIMQLLF YTLGAEVEKG YSGIYQNENT K 

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ69730.1.
RefSeqYP_304310.1. NC_007355.1.

3D structure databases

ProteinModelPortalQ46EG2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269797.Mbar_A0752.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ69730; AAZ69730; Mbar_A0752.
GeneID3626370.
KEGGmba:Mbar_A0752.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0717.
HOGENOMHOG000228599.
KOK01520.
OMAWDAGYEG.
ProtClustDBPRK02253.

Enzyme and pathway databases

BioCycMBAR269797:GHUW-767-MONOMER.
UniPathwayUPA00610; UER00666.

Family and domain databases

HAMAPMF_00635. dUTPase_arch.
InterProIPR008180. dUTP_pyroPase.
IPR023537. dUTP_pyroPase_arch.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDUT_METBF
AccessionPrimary (citable) accession number: Q46EG2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways