Q46E77 (PYLS_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrrolysine--tRNA ligase
EC=6.1.1.26

Alternative name(s):
Pyrrolysine--tRNA(Pyl) ligase
Pyrrolysyl-tRNA synthetase
Short name=PylRS

Gene names

Name:	pylS
Ordered Locus Names:	Mbar_A0839

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG. Ref.2
Catalytic activity	ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl). HAMAP MF_01573
Subcellular location	Cytoplasm HAMAP MF_01573.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Pyrrolysine--tRNA ligase HAMAP MF_01573		PRO_0000260450

Sequences

	Sequence		Length	Mass (Da)
	Q46E77 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 3AA51387F53F8350	FASTA	419	47,690
	10 20 30 40 50 60 MDKKPLDVLI SATGLWMSRT GTLHKIKHYE VSRSKIYIEM ACGDHLVVNN SRSCRTARAF 70 80 90 100 110 120 RHHKYRKTCK RCRVSDEDIN NFLTRSTEGK TSVKVKVVSA PKVKKAMPKS VSRAPKPLEN 130 140 150 160 170 180 PVSAKASTDT SRSVPSPAKS TPNSPVPTSA PAPSLTRSQL DRVEALLSPE DKISLNIAKP 190 200 210 220 230 240 FRELESELVT RRKNDFQRLY TNDREDYLGK LERDITKFFV DRDFLEIKSP ILIPAEYVER 250 260 270 280 290 300 MGINNDTELS KQIFRVDKNL CLRPMLAPTL YNYLRKLDRI LPDPIKIFEV GPCYRKESDG 310 320 330 340 350 360 KEHLEEFTMV NFCQMGSGCT RENLESLIKE FLDYLEIDFE IVGDSCMVYG DTLDIMHGDL 370 380 390 400 410 ELSSAVVGPV PLDREWGIDK PWIGAGFGLE RLLKVMHGFK NIKRASRSES YYNGISTNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.
[2]	"An aminoacyl-tRNA synthetase that specifically activates pyrrolysine." Polycarpo C., Ambrogelly A., Berube A., Winbush S.M., McCloskey J.A., Crain P.F., Wood J.L., Soell D. Proc. Natl. Acad. Sci. U.S.A. 101:12450-12454(2004) [PubMed: 15314242] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69815.1.
RefSeq	YP_304395.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46E77.
SMR	Q46E77. Positions 153-419.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46E77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3625577.
GenomeReviews	Gene locus Mbar_A0839 in contig CP000099_GR.
KEGG	mba:Mbar_A0839.
NMPDR	fig|269797.3.peg.315.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05099.
HOGENOM	HBG459849.
OMA	FCQMGSG.
ProtClustDB	PRK09537.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0839-MONOMER.
Family and domain databases
HAMAP	MF_01573. Pyl_tRNA_synth. [Tree]
InterPro	IPR018150. aa-tRNA-synt_II-like. IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR012739. Pyrrolysyl-tRNA_ligase. IPR023877. Pyrrolysyl-tRNA_ligase_C. IPR023878. Pyrrolysyl-tRNA_ligase_N. [Graphical view]
KO	K11627.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
TIGRFAMs	TIGR02367. PylS_Cterm. 1 hit. TIGR03912. PylS_Nterm. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYLS_METBF

Accession

Primary (citable) accession number: Q46E77

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program