Reviewed,
UniProtKB/Swiss-Prot Q46E77 (PYLS_METBF)
Last modified
June 16, 2009.
Version 33.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Pyrrolysyl-tRNA synthetase EC=6.1.1.26 Alternative name(s): Pyrrolysine--tRNA ligase Short name=PylRS | ||||
| Gene names |
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| Organism | Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 269797 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina |
Protein attributes
| Sequence length | 419 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG. Ref.2 |
| Catalytic activity | ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl). HAMAP MF_01573 |
| Subcellular location | |
| Sequence similarities | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Aminoacyl-tRNA synthetase Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: HAMAP |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 419 | 419 | Pyrrolysyl-tRNA synthetase HAMAP MF_01573 | PRO_0000260450 | |||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [2] | "An aminoacyl-tRNA synthetase that specifically activates pyrrolysine." Polycarpo C., Ambrogelly A., Berube A., Winbush S.M., McCloskey J.A., Crain P.F., Wood J.L., Soell D. Proc. Natl. Acad. Sci. U.S.A. 101:12450-12454(2004) [PubMed: 15314242] [Abstract] Cited for: FUNCTION. |
Cross-references
Sequence databases | |
|---|---|
| CP000099 Genomic DNA. Translation: AAZ69815.1. | |
| RefSeq | YP_304395.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 3625577. |
| GenomeReviews | Gene locus Mbar_A0839 in contig CP000099_GR. |
| KEGG | mba:Mbar_A0839. |
| NMPDR | fig|269797.3.peg.315. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q46E77. |
| OMA | Q46E77. SGCTREN. |
Enzyme and pathway databases | |
| BioCyc | MBAR269797:MBAR_A0839-MON. |
Family and domain databases | |
| HAMAP | MF_01573. [Tree] |
| InterPro | IPR018150. aa-tRNA-synt_II-like. IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR012739. PylS. [Graphical view] |
| PANTHER | PTHR22594. aa-tRNA-synt_II. 1 hit. |
| Pfam | PF00587. tRNA-synt_2b. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR02367. PylS. 1 hit. |
| PROSITE | PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PYLS_METBF | ||||||||
| Accession | Primary (citable) accession number: Q46E77 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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