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Q46E77 (PYLS_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyrrolysine--tRNA ligase

EC=6.1.1.26
Alternative name(s):
Pyrrolysine--tRNA(Pyl) ligase
Pyrrolysyl-tRNA synthetase
Short name=PylRS
Gene names
Name:pylS
Ordered Locus Names:Mbar_A0839
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG. Ref.2

Catalytic activity

ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl). HAMAP MF_01573

Subcellular location

Cytoplasm HAMAP MF_01573.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Pyrrolysine--tRNA ligase HAMAP MF_01573
PRO_0000260450

Sequences

Sequence LengthMass (Da)Tools
Q46E77 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 3AA51387F53F8350

FASTA41947,690
        10         20         30         40         50         60 
MDKKPLDVLI SATGLWMSRT GTLHKIKHYE VSRSKIYIEM ACGDHLVVNN SRSCRTARAF 

        70         80         90        100        110        120 
RHHKYRKTCK RCRVSDEDIN NFLTRSTEGK TSVKVKVVSA PKVKKAMPKS VSRAPKPLEN 

       130        140        150        160        170        180 
PVSAKASTDT SRSVPSPAKS TPNSPVPTSA PAPSLTRSQL DRVEALLSPE DKISLNIAKP 

       190        200        210        220        230        240 
FRELESELVT RRKNDFQRLY TNDREDYLGK LERDITKFFV DRDFLEIKSP ILIPAEYVER 

       250        260        270        280        290        300 
MGINNDTELS KQIFRVDKNL CLRPMLAPTL YNYLRKLDRI LPDPIKIFEV GPCYRKESDG 

       310        320        330        340        350        360 
KEHLEEFTMV NFCQMGSGCT RENLESLIKE FLDYLEIDFE IVGDSCMVYG DTLDIMHGDL 

       370        380        390        400        410 
ELSSAVVGPV PLDREWGIDK PWIGAGFGLE RLLKVMHGFK NIKRASRSES YYNGISTNL 

« Hide

References

« Hide 'large scale' references
[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.
[2]"An aminoacyl-tRNA synthetase that specifically activates pyrrolysine."
Polycarpo C., Ambrogelly A., Berube A., Winbush S.M., McCloskey J.A., Crain P.F., Wood J.L., Soell D.
Proc. Natl. Acad. Sci. U.S.A. 101:12450-12454(2004) [PubMed: 15314242] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ69815.1.
RefSeqYP_304395.1. NC_007355.1.

3D structure databases

ProteinModelPortalQ46E77.
SMRQ46E77. Positions 153-419.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ46E77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3625577.
GenomeReviewsGene locus Mbar_A0839 in contig CP000099_GR.
KEGGmba:Mbar_A0839.
NMPDRfig|269797.3.peg.315.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG05099.
HOGENOMHBG459849.
OMAFCQMGSG.
ProtClustDBPRK09537.

Enzyme and pathway databases

BioCycMBAR269797:MBAR_A0839-MONOMER.

Family and domain databases

HAMAPMF_01573. Pyl_tRNA_synth.
[Tree]
InterProIPR018150. aa-tRNA-synt_II-like.
IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR012739. Pyrrolysyl-tRNA_ligase.
IPR023877. Pyrrolysyl-tRNA_ligase_C.
IPR023878. Pyrrolysyl-tRNA_ligase_N.
[Graphical view]
KOK11627.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PfamPF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
TIGRFAMsTIGR02367. PylS_Cterm. 1 hit.
TIGR03912. PylS_Nterm. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYLS_METBF
AccessionPrimary (citable) accession number: Q46E77
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: September 13, 2005
Last modified: January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families