Q46E56 (PUR5_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	Mbar_A0862

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_A
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_A.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_A		PRO_0000258438

Sequences

	Sequence		Length	Mass (Da)
	Q46E56 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 55D7717444090455	FASTA	333	36,284
	10 20 30 40 50 60 MSEKHLTYAD SGVDITKEEK TVKTLIEKLS YVRKGMGAPL TGIGHYAGLL DFGEYALALT 70 80 90 100 110 120 TDGVGSKVLI ANEMQRWNTV GIDCIAMNVN DLLAIGAEPV AFVDYLALEK HEEGFAAQIG 130 140 150 160 170 180 EGLVKGAEIS RMSIVGGETA TLPEIIKGFD LAGTCLGIVR KDEIVEGEKV RVGDVIVGVP 190 200 210 220 230 240 STGVHSNGYT LVRKIIEESR YSYHDPCPYD NSKMIGDELL TPTRIYIEIL DVLKACEVHG 250 260 270 280 290 300 LAHITGSGLL KLRRVTKLGF DFYDPLEPQE IFKFLQKEGG VEDLEMYRTF NMGMGFLVIL 310 320 330 PEKDAAKAAK ITGGKIVGKI VESGIRVKDL VIE

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69836.1.
RefSeq	YP_304416.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46E56.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46E56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3627274.
GenomeReviews	Gene locus Mbar_A0862 in contig CP000099_GR.
KEGG	mba:Mbar_A0862.
NMPDR	fig|269797.3.peg.336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04189.
HOGENOM	HBG531222.
OMA	GIDMIAM.
PhylomeDB	Q46E56.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0862-MONOMER.
Family and domain databases
HAMAP	MF_00741_A. AIRS_A. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_METBF

Accession

Primary (citable) accession number: Q46E56

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 13, 2005
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program