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Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Shikimate-3-phosphateUniRule annotation
Binding sitei131 – 1311PhosphoenolpyruvateUniRule annotation
Binding sitei204 – 2041Shikimate-3-phosphateUniRule annotation
Active sitei324 – 3241Proton acceptorUniRule annotation
Binding sitei351 – 3511Shikimate-3-phosphateUniRule annotation
Active sitei352 – 3521Proton donorUniRule annotation
Binding sitei355 – 3551PhosphoenolpyruvateUniRule annotation
Binding sitei396 – 3961PhosphoenolpyruvateUniRule annotation

GO - Molecular functioni

  1. 3-phosphoshikimate 1-carboxyvinyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationImported

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesisUniRule annotation

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-905-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:Mbar_A0891Imported
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)Imported
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269797.Mbar_A0891.

Structurei

3D structure databases

ProteinModelPortaliQ46E27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 342Shikimate-3-phosphate bindingUniRule annotation
Regioni101 – 1044PhosphoenolpyruvateUniRule annotation
Regioni176 – 1783Shikimate-3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247372.
KOiK00800.
OMAiHDHRLVM.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46E27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNPIRNRRD LIHMRVSIDK SSIKGEVFAP PSKSYTHRAV TLAALSKEST
60 70 80 90 100
VRHPLISADT LATVRASEMF GALVEREEDR LIIHGINGKP NVPDDVIDAA
110 120 130 140 150
NSGTTLRFMT AVAALTDGIT VLTGDASLRT RPNGPLLEVL NRLGVKACST
160 170 180 190 200
RGNERAPLVV KGGLKGQDVS IDGSISSQFI SALLITCPLA ENSTILSITG
210 220 230 240 250
KIKSRPYVDI TLEMLELAGV KVHIDDSNGT RFIIPGKQKY DFKDYTVPGD
260 270 280 290 300
FSSASYLLAA AAMTDGSEVT VKNLFPSKQG DKVIIETLKQ MGADITWDKE
310 320 330 340 350
AGNVTVKGGR QLKAITFDAG ANPDLVPTVA VLAAVAKGTS RIENAEHVRY
360 370 380 390 400
KETDRLRALA TELPKLGVDL KEERDSLTIT GGKLHGASVH GWDDHRIVMA
410 420 430 440
LSVAGIVAGN TKIDTTESAS ISYPEFFKDM RSLGAKIKDF SKE
Length:443
Mass (Da):47,678
Last modified:September 12, 2005 - v1
Checksum:i0922FCDE57D7B94C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ69865.1.
RefSeqiYP_304445.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ69865; AAZ69865; Mbar_A0891.
GeneIDi3625936.
KEGGimba:Mbar_A0891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ69865.1.
RefSeqiYP_304445.1. NC_007355.1.

3D structure databases

ProteinModelPortaliQ46E27.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A0891.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ69865; AAZ69865; Mbar_A0891.
GeneIDi3625936.
KEGGimba:Mbar_A0891.

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247372.
KOiK00800.
OMAiHDHRLVM.

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-905-MONOMER.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804Imported.

Entry informationi

Entry nameiQ46E27_METBF
AccessioniPrimary (citable) accession number: Q46E27
Entry historyi
Integrated into UniProtKB/TrEMBL: September 12, 2005
Last sequence update: September 12, 2005
Last modified: March 31, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.