Q46E19 (DP2L_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	Mbar_A0899

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	1146 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1146	1146	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294692

Sequences

	Sequence		Length	Mass (Da)
	Q46E19 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 867140FA2D6E386A	FASTA	1,146	128,094
	10 20 30 40 50 60 MGETIASKEM HEYFDELEAR LKKAIEIANT ARARGGDPKP VVEIPLAKDL ADRVENLIGV 70 80 90 100 110 120 EGVAAKIRVL EEKMSREECA LEIGRQVAEG EVGNFATKKD AVEAAIRVSM AVITEGVVAA 130 140 150 160 170 180 PIEGINKVEL GKNDDGSEYI RIFYSGPIRS AGGTAQALSV LVGDYVRRGI GIDRYKPRPE 190 200 210 220 230 240 EVERYVEEIV LYKRVASLQY MPSEDEIRLI VKNCPVCIDG DPTEEAEVEG HRNLERIGTN 250 260 270 280 290 300 RIRGGMCLVL AEGLALKAPK VKKHVNKLNM DGWDWLDILI GGAKTGGDEE DEKKNKIKPK 310 320 330 340 350 360 DKYIRDLIAG RPVFSHPSRP GGFRLRYGRS RNTSFAAAGI NPSTMVLLDD FITNGTQLKV 370 380 390 400 410 420 ERPGKAAAMS AVDSIEGPTV RLYSGDLVRI DDIKEAYELR SQVEVIVDIG EILINYGDFL 430 440 450 460 470 480 ENNHPLMPSP YVFEWWRYDY EAACSEIIPE DELKDPSSAL ALRLAEEYDV PLHPKFTYLW 490 500 510 520 530 540 HDINRTEFEA LRKFVVGRGN FSVEDEILRL PLKACIENGV KLILEKLLVL HRVKADTILI 550 560 570 580 590 600 KEALPFILCL GLDCHLKEKA PMPDTDNMVN ATAFLSGFKV LPRAPSRIGA RMGRPEKANL 610 620 630 640 650 660 RKMSPAAQVL FPIGNAGGLT RNLVSASNYS VSMNGKIGEI EVEMGIRECP ACGKETYFWR 670 680 690 700 710 720 CDCGEYTRPK LFCPRCKIEV RNSETCPKCG RKPTSVSNVK LDFRSIYKRA FENVGEREKM 730 740 750 760 770 780 DLIKGVKRLM NGQMTPEPLE KGILRAKHDV YIFKDGTVRY DMSDIPLTHV RADEIGITAA 790 800 810 820 830 840 KLRELGYVED IYGNPLERDD QIVCLKVQDL VISYDGGGYM LRTAQYVDDL LVKYYGVEPY 850 860 870 880 890 900 YNAKTIQDLV GVLLIGLAPH TSAGVLGRLI GFTRASVGYA HPFFHASKRR NCDGDEDCVM 910 920 930 940 950 960 LLMDGILNFS RAYLPEKRGG KMDAPLVLTT RIDPKEVDKE AHNIDLPARY PLEFYRATQE 970 980 990 1000 1010 1020 IKNPTELEGI MDLVSSRLGT PEQYEHFMFT HDTSDIAAGP LNSSYKTLGS MIDKMEAQLS 1030 1040 1050 1060 1070 1080 LANKIRAVDA PDVAERVLKS HFLPDLLGNL RSFSRQRMRC IKCGAKFRRP PLTGACPKCG 1090 1100 1110 1120 1130 1140 GNVVLTVHEG AVRKYLEISK EIGKRYGVSS YTQQRIELLD KDICSLFENH RVKQLGISDF MSGSAR

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69873.1.
RefSeq	YP_304453.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46E19.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46E19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3625944.
GenomeReviews	Gene locus Mbar_A0899 in contig CP000099_GR.
KEGG	mba:Mbar_A0899.
NMPDR	fig|269797.3.peg.460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04008.
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK04023.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0899-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METBF

Accession

Primary (citable) accession number: Q46E19

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program