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Q46E16

- RBL_METBF

UniProt

Q46E16 - RBL_METBF

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei151 – 1511Proton acceptorUniRule annotation
    Binding sitei153 – 1531SubstrateUniRule annotation
    Metal bindingi177 – 1771Magnesium; via carbamate groupUniRule annotation
    Metal bindingi179 – 1791MagnesiumUniRule annotation
    Metal bindingi180 – 1801MagnesiumUniRule annotation
    Active sitei270 – 2701Proton acceptorUniRule annotation
    Binding sitei271 – 2711SubstrateUniRule annotation
    Binding sitei303 – 3031SubstrateUniRule annotation
    Sitei310 – 3101Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. oxidoreductase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase, Oxidoreductase

    Keywords - Biological processi

    Carbon dioxide fixation

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-916-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:Mbar_A0902
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Ribulose bisphosphate carboxylasePRO_1000065430Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei177 – 1771N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi269797.Mbar_A0902.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46E16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni354 – 3563Substrate bindingUniRule annotation
    Regioni376 – 3794Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1850.
    HOGENOMiHOG000230831.
    KOiK01601.
    OMAiFTQDWAS.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01133. RuBisCO_L_type3.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q46E16-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQRDYIDAGY SPKDTDLICE FHIEPSAGVN FEEAATHMAG ESSIDSWTEI    50
    ATLSPELAAR LKPHVFYMDE DAQTVRVAYS EELFELGSVP QVLSAVAGNI 100
    LSMKIVDNLR LQDIAFPKSM LREFKGPGFG LSGIRELTGV QDRPLIGTIV 150
    KPKVGLTSEK HAEVAYNSFA GGCDLVKDDE NLTDQKFNKF EKRAELTLKL 200
    AEKAESETGE RKMYLCNITA PTCKEMIRRM NILKDLGASY AMIDIVPAGW 250
    TALQTLREEA DDAGLALHAH RCMHSAYTRN PRHGISMLVV AKLCRLIGLD 300
    QLHIGTVVGK MHGEKHEVLS LRDECVLDNV PADESQHVLA QDWGGLKPMF 350
    PVASGGLAPT MIPDLYTIFG RDVIMQFGGG IHAHPMGTKA GAAACRQALE 400
    ASLEGVSLQE YAKNHRELEA AINKWLKK 428
    Length:428
    Mass (Da):47,061
    Last modified:September 13, 2005 - v1
    Checksum:i74DCE0F7F03502E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ69876.1.
    RefSeqiWP_011305925.1. NC_007355.1.
    YP_304456.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ69876; AAZ69876; Mbar_A0902.
    GeneIDi3625947.
    KEGGimba:Mbar_A0902.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ69876.1 .
    RefSeqi WP_011305925.1. NC_007355.1.
    YP_304456.1. NC_007355.1.

    3D structure databases

    ProteinModelPortali Q46E16.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A0902.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ69876 ; AAZ69876 ; Mbar_A0902 .
    GeneIDi 3625947.
    KEGGi mba:Mbar_A0902.

    Phylogenomic databases

    eggNOGi COG1850.
    HOGENOMi HOG000230831.
    KOi K01601.
    OMAi FTQDWAS.

    Enzyme and pathway databases

    BioCyci MBAR269797:GHUW-916-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01133. RuBisCO_L_type3.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.

    Entry informationi

    Entry nameiRBL_METBF
    AccessioniPrimary (citable) accession number: Q46E16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3