Q46DZ7 (AROD_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	Mbar_A0922

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_1000043171
Sites
	Active site	135	1	Proton acceptor By similarity
	Active site	162	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46DZ7 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 465B23712D234BFD	FASTA	242	26,245
	10 20 30 40 50 60 MIHIGQLDLE KKAAVVAVIL EKSLEISRKA AKMGADLLEI RLDLLGIRDL ETAAETIRKV 70 80 90 100 110 120 KSETGLPVIL TNRSITEGGK WEGKEADRIE LLTNLISLKD GPDAVDIELS AGREARDQVI 130 140 150 160 170 180 KAAKAHGKTV IVSSHDFSRT PAFQEMKTIL EEAFLAGADI AKLAVMPQSR RDVLNLLRVA 190 200 210 220 230 240 LDAREAGNAV CTIAMGRLGK HTRVIAPFYG SVLTYAAVNS EVSAAPGQFQ VDEVKKILEL LE

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69895.1.
RefSeq	YP_304475.1. NC_007355.1.
3D structure databases
HSSP	HSSP built from PDB template 2GPT based on UniProtKB Q9SQT8.
ProteinModelPortal	Q46DZ7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46DZ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3627302.
GenomeReviews	Gene locus Mbar_A0922 in contig CP000099_GR.
KEGG	mba:Mbar_A0922.
NMPDR	fig|269797.3.peg.482.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG12575.
HOGENOM	HBG732926.
OMA	VARRTVI.
PhylomeDB	Q46DZ7.
ProtClustDB	CLSK450616.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0922-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_METBF

Accession

Primary (citable) accession number: Q46DZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program