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Q46DY5

- FAEHP_METBF

UniProt

Q46DY5 - FAEHP_METBF

Protein

Bifunctional enzyme Fae/Hps

Gene

faeB-hpsB

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H4MPT) to 5,10-methylenetetrahydromethanopterin.1 PublicationUniRule annotation
    Catalyzes the formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.1 PublicationUniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydromethanopterin = tetrahydromethanopterin + formaldehyde.UniRule annotation
    D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei17 – 171Proton donorUniRule annotation
    Binding sitei19 – 191SubstrateUniRule annotation
    Binding sitei48 – 481Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei66 – 661SubstrateUniRule annotation
    Binding sitei68 – 681SubstrateUniRule annotation
    Binding sitei83 – 831SubstrateUniRule annotation

    GO - Molecular functioni

    1. aldehyde-lyase activity Source: UniProtKB-HAMAP
    2. carbon-nitrogen lyase activity Source: UniProtKB-HAMAP
    3. orotidine-5'-phosphate decarboxylase activity Source: InterPro

    GO - Biological processi

    1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
    2. carbohydrate biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-952-MONOMER.
    UniPathwayiUPA00293.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional enzyme Fae/HpsUniRule annotation
    Including the following 2 domains:
    Formaldehyde-activating enzymeUniRule annotation (EC:4.3.3.-UniRule annotation)
    Short name:
    FaeUniRule annotation
    3-hexulose-6-phosphate synthaseUniRule annotation (EC:4.1.2.43UniRule annotation)
    Short name:
    HPSUniRule annotation
    Alternative name(s):
    D-arabino-3-hexulose-6-phosphate formaldehyde lyaseUniRule annotation
    Gene namesi
    Name:faeB-hpsB
    Ordered Locus Names:Mbar_A0935
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392Bifunctional enzyme Fae/HpsPRO_0000235168Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi269797.Mbar_A0935.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46DY5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 161161Formaldehyde-activating enzymeAdd
    BLAST
    Regioni162 – 3922313-hexulose-6-phosphate synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the formaldehyde-activating enzyme family.UniRule annotation
    In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1795.
    HOGENOMiHOG000286408.
    KOiK13812.
    OMAiYNYGATK.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.30.230.60. 1 hit.
    HAMAPiMF_01268. Fae_Hps.
    InterProiIPR013785. Aldolase_TIM.
    IPR020868. Bifunctional_enzyme_fae/hps.
    IPR014826. HCHO-activating_enzyme.
    IPR001754. OMPdeCOase_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view]
    PfamiPF08714. Fae. 1 hit.
    PF00215. OMPdecase. 1 hit.
    [Graphical view]
    SMARTiSM00934. OMPdecase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    SSF54211. SSF54211. 1 hit.
    TIGRFAMsiTIGR03126. one_C_fae. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q46DY5-1 [UniParc]FASTAAdd to Basket

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    MFQIGEALMG QGAELAHVDL MIGDKGGPVG QAFANGLTQL SVGHTPLLSV    50
    IRPNLPPKPS TLIIPKVTVK NMEQAGKIFG PAQAAVAKAV ADSVEEGVIS 100
    KDQVEEIVIV ASVFIHPDAQ DYNKIYRYNY GATKLAIKRA LGGFPDINTV 150
    LEESNKSTHA IMGFKVTRLW DPPYLQVAFD NPDIEFVQSA ISQIPKSDHV 200
    IIEAGTPLIK RYGMDVISRI REVRPDAFIV ADLKTLDTGN LEARMVADAA 250
    GDAIVVSALA PISTIDKLIE EAHKTGIYAV MDTLNQQDPI SVLKQLKVMP 300
    DVIELHRGID IEATEHAWGN IAEIKKIAPK ILVAVAGGVR LDKVPVALGQ 350
    GADILVVGRA ITNSKDVREV AEQFINSLNK PEIDQFRVMT DF 392
    Length:392
    Mass (Da):42,415
    Last modified:September 13, 2005 - v1
    Checksum:iAE132C4FBBAC00A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ69907.1.
    RefSeqiYP_304487.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ69907; AAZ69907; Mbar_A0935.
    GeneIDi3627315.
    KEGGimba:Mbar_A0935.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ69907.1 .
    RefSeqi YP_304487.1. NC_007355.1.

    3D structure databases

    ProteinModelPortali Q46DY5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A0935.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ69907 ; AAZ69907 ; Mbar_A0935 .
    GeneIDi 3627315.
    KEGGi mba:Mbar_A0935.

    Phylogenomic databases

    eggNOGi COG1795.
    HOGENOMi HOG000286408.
    KOi K13812.
    OMAi YNYGATK.

    Enzyme and pathway databases

    UniPathwayi UPA00293 .
    BioCyci MBAR269797:GHUW-952-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.30.230.60. 1 hit.
    HAMAPi MF_01268. Fae_Hps.
    InterProi IPR013785. Aldolase_TIM.
    IPR020868. Bifunctional_enzyme_fae/hps.
    IPR014826. HCHO-activating_enzyme.
    IPR001754. OMPdeCOase_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view ]
    Pfami PF08714. Fae. 1 hit.
    PF00215. OMPdecase. 1 hit.
    [Graphical view ]
    SMARTi SM00934. OMPdecase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    SSF54211. SSF54211. 1 hit.
    TIGRFAMsi TIGR03126. one_C_fae. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.
    2. "Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis."
      Goenrich M., Thauer R.K., Yurimoto H., Kato N.
      Arch. Microbiol. 184:41-48(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CHARACTERIZATION, PATHWAY.

    Entry informationi

    Entry nameiFAEHP_METBF
    AccessioniPrimary (citable) accession number: Q46DY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3