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Q46DY5

- FAEHP_METBF

UniProt

Q46DY5 - FAEHP_METBF

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Protein
Bifunctional enzyme Fae/Hps
Gene
faeB-hpsB, Mbar_A0935
Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H4MPT) to 5,10-methylenetetrahydromethanopterin.1 Publication
Catalyzes the formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.1 Publication

Catalytic activityi

5,10-methylenetetrahydromethanopterin = tetrahydromethanopterin + formaldehyde.UniRule annotation
D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei17 – 171Proton donor By similarity
Binding sitei19 – 191Substrate By similarity
Binding sitei48 – 481Substrate; via carbonyl oxygen By similarity
Binding sitei66 – 661Substrate By similarity
Binding sitei68 – 681Substrate By similarity
Binding sitei83 – 831Substrate By similarity

GO - Molecular functioni

  1. aldehyde-lyase activity Source: UniProtKB-HAMAP
  2. carbon-nitrogen lyase activity Source: UniProtKB-HAMAP
  3. orotidine-5'-phosphate decarboxylase activity Source: InterPro

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. carbohydrate biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-952-MONOMER.
UniPathwayiUPA00293.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme Fae/Hps
Including the following 2 domains:
Formaldehyde-activating enzyme (EC:4.3.3.-)
Short name:
Fae
3-hexulose-6-phosphate synthase (EC:4.1.2.43)
Short name:
HPS
Alternative name(s):
D-arabino-3-hexulose-6-phosphate formaldehyde lyase
Gene namesi
Name:faeB-hpsB
Ordered Locus Names:Mbar_A0935
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Bifunctional enzyme Fae/HpsUniRule annotation
PRO_0000235168Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A0935.

Structurei

3D structure databases

ProteinModelPortaliQ46DY5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 161161Formaldehyde-activating enzymeUniRule annotation
Add
BLAST
Regioni162 – 3922313-hexulose-6-phosphate synthaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the formaldehyde-activating enzyme family.
In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.

Phylogenomic databases

eggNOGiCOG1795.
HOGENOMiHOG000286408.
KOiK13812.
OMAiYNYGATK.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.30.230.60. 1 hit.
HAMAPiMF_01268. Fae_Hps.
InterProiIPR013785. Aldolase_TIM.
IPR020868. Bifunctional_enzyme_fae/hps.
IPR014826. HCHO-activating_enzyme.
IPR001754. OMPdeCOase_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF08714. Fae. 1 hit.
PF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF54211. SSF54211. 1 hit.
TIGRFAMsiTIGR03126. one_C_fae. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46DY5-1 [UniParc]FASTAAdd to Basket

« Hide

MFQIGEALMG QGAELAHVDL MIGDKGGPVG QAFANGLTQL SVGHTPLLSV    50
IRPNLPPKPS TLIIPKVTVK NMEQAGKIFG PAQAAVAKAV ADSVEEGVIS 100
KDQVEEIVIV ASVFIHPDAQ DYNKIYRYNY GATKLAIKRA LGGFPDINTV 150
LEESNKSTHA IMGFKVTRLW DPPYLQVAFD NPDIEFVQSA ISQIPKSDHV 200
IIEAGTPLIK RYGMDVISRI REVRPDAFIV ADLKTLDTGN LEARMVADAA 250
GDAIVVSALA PISTIDKLIE EAHKTGIYAV MDTLNQQDPI SVLKQLKVMP 300
DVIELHRGID IEATEHAWGN IAEIKKIAPK ILVAVAGGVR LDKVPVALGQ 350
GADILVVGRA ITNSKDVREV AEQFINSLNK PEIDQFRVMT DF 392
Length:392
Mass (Da):42,415
Last modified:September 13, 2005 - v1
Checksum:iAE132C4FBBAC00A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ69907.1.
RefSeqiYP_304487.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ69907; AAZ69907; Mbar_A0935.
GeneIDi3627315.
KEGGimba:Mbar_A0935.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ69907.1 .
RefSeqi YP_304487.1. NC_007355.1.

3D structure databases

ProteinModelPortali Q46DY5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269797.Mbar_A0935.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ69907 ; AAZ69907 ; Mbar_A0935 .
GeneIDi 3627315.
KEGGi mba:Mbar_A0935.

Phylogenomic databases

eggNOGi COG1795.
HOGENOMi HOG000286408.
KOi K13812.
OMAi YNYGATK.

Enzyme and pathway databases

UniPathwayi UPA00293 .
BioCyci MBAR269797:GHUW-952-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
3.30.230.60. 1 hit.
HAMAPi MF_01268. Fae_Hps.
InterProi IPR013785. Aldolase_TIM.
IPR020868. Bifunctional_enzyme_fae/hps.
IPR014826. HCHO-activating_enzyme.
IPR001754. OMPdeCOase_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
Pfami PF08714. Fae. 1 hit.
PF00215. OMPdecase. 1 hit.
[Graphical view ]
SMARTi SM00934. OMPdecase. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
SSF54211. SSF54211. 1 hit.
TIGRFAMsi TIGR03126. one_C_fae. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.
  2. "Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis."
    Goenrich M., Thauer R.K., Yurimoto H., Kato N.
    Arch. Microbiol. 184:41-48(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CHARACTERIZATION, PATHWAY.

Entry informationi

Entry nameiFAEHP_METBF
AccessioniPrimary (citable) accession number: Q46DY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: September 13, 2005
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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