Q46DY5 (FAEHP_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional enzyme fae/hps

Including the following 2 domains:

1. Formaldehyde-activating enzyme
   Short name=Fae
   EC=4.3.3.-
2. 3-hexulose-6-phosphate synthase
   Short name=HPS
   EC=4.1.2.43
   Alternative name(s):
   D-arabino-3-hexulose-6-phosphate formaldehyde lyase

Gene names

Name:	faeB-hpsB
Ordered Locus Names:	Mbar_A0935

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina barkeri

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H4MPT) to 5,10-methylenetetrahydromethanopterin. Ref.2 Catalyzes the formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate. Ref.2
Catalytic activity	5,10-methylenetetrahydromethanopterin = tetrahydromethanopterin + formaldehyde. HAMAP-Rule MF_01268 D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde. HAMAP-Rule MF_01268
Pathway	Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis. Ref.2
Sequence similarities	In the N-terminal section; belongs to the formaldehyde-activating enzyme family. In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	'de novo' pyrimidine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro carbohydrate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	aldehyde-lyase activity Inferred from electronic annotation. Source: HAMAP carbon-nitrogen lyase activity Inferred from electronic annotation. Source: HAMAP orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Bifunctional enzyme fae/hps HAMAP-Rule MF_01268		PRO_0000235168
Regions
	Region	1 – 161	161	Formaldehyde-activating enzyme HAMAP-Rule MF_01268
	Region	162 – 392	231	3-hexulose-6-phosphate synthase HAMAP-Rule MF_01268
Sites
	Active site	17	1	Proton donor By similarity
	Binding site	19	1	Substrate By similarity
	Binding site	48	1	Substrate; via carbonyl oxygen By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	68	1	Substrate By similarity
	Binding site	83	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46DY5 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: AE132C4FBBAC00A1	FASTA	392	42,415
	10 20 30 40 50 60 MFQIGEALMG QGAELAHVDL MIGDKGGPVG QAFANGLTQL SVGHTPLLSV IRPNLPPKPS 70 80 90 100 110 120 TLIIPKVTVK NMEQAGKIFG PAQAAVAKAV ADSVEEGVIS KDQVEEIVIV ASVFIHPDAQ 130 140 150 160 170 180 DYNKIYRYNY GATKLAIKRA LGGFPDINTV LEESNKSTHA IMGFKVTRLW DPPYLQVAFD 190 200 210 220 230 240 NPDIEFVQSA ISQIPKSDHV IIEAGTPLIK RYGMDVISRI REVRPDAFIV ADLKTLDTGN 250 260 270 280 290 300 LEARMVADAA GDAIVVSALA PISTIDKLIE EAHKTGIYAV MDTLNQQDPI SVLKQLKVMP 310 320 330 340 350 360 DVIELHRGID IEATEHAWGN IAEIKKIAPK ILVAVAGGVR LDKVPVALGQ GADILVVGRA 370 380 390 ITNSKDVREV AEQFINSLNK PEIDQFRVMT DF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.
[2]	"Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis." Goenrich M., Thauer R.K., Yurimoto H., Kato N. Arch. Microbiol. 184:41-48(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CHARACTERIZATION, PATHWAY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69907.1.
RefSeq	YP_304487.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46DY5.
ModBase	Search...
Protein-protein interaction databases
STRING	269797.Mbar_A0935.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ69907; AAZ69907; Mbar_A0935.
GeneID	3627315.
KEGG	mba:Mbar_A0935.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1795.
HOGENOM	HOG000286408.
KO	K13812.
OMA	YNYGATK.
ProtClustDB	PRK13307.
Enzyme and pathway databases
BioCyc	MBAR269797:GHUW-952-MONOMER.
UniPathway	UPA00293.
Family and domain databases
Gene3D	3.20.20.70. 1 hit. 3.30.230.60. 1 hit.
HAMAP	MF_01268. Fae_Hps.
InterPro	IPR013785. Aldolase_TIM. IPR020868. Bifunctional_enzyme_fae/hps. IPR014826. HCHO-activating_enzyme. IPR001754. OMPdeCOase_dom. IPR020568. Ribosomal_S5_D2-typ_fold. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF08714. Fae. 1 hit. PF00215. OMPdecase. 1 hit. [Graphical view]
SMART	SM00934. OMPdecase. 1 hit. [Graphical view]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR03126. one_C_fae. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FAEHP_METBF

Accession

Primary (citable) accession number: Q46DY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	May 29, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program