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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPUniRule annotation1
Active sitei301Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi276 – 280ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:Mbar_A0961
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002648011 – 331RNA 3'-terminal phosphate cyclaseAdd BLAST331

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A0961.

Structurei

3D structure databases

ProteinModelPortaliQ46DV9.
SMRiQ46DV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04125. Archaea.
COG0430. LUCA.
HOGENOMiHOG000015264.
KOiK01974.
OMAiANRPKPG.
OrthoDBiPOG093Z05RD.

Family and domain databases

Gene3Di3.65.10.20. 1 hit.
HAMAPiMF_00200. RTC. 1 hit.
InterProiView protein in InterPro
IPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
PANTHERiPTHR11096. PTHR11096. 1 hit.
PTHR11096:SF2. PTHR11096:SF2. 1 hit.
PfamiView protein in Pfam
PF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiView protein in PROSITE
PS01287. RTC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46DV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEIDGSYGE GGGQLVRTAV ALSAVTGQGI RITNIRKNRP SPGLKQQHLK
60 70 80 90 100
ALETAARICR AQISGLFPGS TEISFVPVEI EGGKYDIDIG TAGSITLLLQ
110 120 130 140 150
CIMPALPFAK EKVELTIKGG TDVAWSPTID YLQHVTFRAL EQLGYSGSIT
160 170 180 190 200
LKEHGYYPKG GGKVSAYFKP CRLREFHFLK EKEDIKGSSH ASNLPAHVPL
210 220 230 240 250
RQAEAASKRL MEAGYPSLIE TQSFEAFSIG SGITLWIGYI GGSALGERGL
260 270 280 290 300
PAEKVGKNAA DEIIPELRSG ASVDTHLADQ LIPYMALAGN SSYTVRELSL
310 320 330
HTTTNIWVTE QFLDVKFRIE KKEGLFEVSV S
Length:331
Mass (Da):35,884
Last modified:September 13, 2005 - v1
Checksum:i706A23532B651569
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ69933.1.
RefSeqiWP_011305982.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ69933; AAZ69933; Mbar_A0961.
GeneIDi3626457.
KEGGimba:Mbar_A0961.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRTCA_METBF
AccessioniPrimary (citable) accession number: Q46DV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 13, 2005
Last modified: June 7, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families