Reviewed, UniProtKB/Swiss-Prot Q46DV9 (RTCA_METBF)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Mbar_A0961

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000264801
Sites
	Active site	301	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46DV9-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 706A23532B651569	FASTA	331	35,884
	10 20 30 40 50 60 MLEIDGSYGE GGGQLVRTAV ALSAVTGQGI RITNIRKNRP SPGLKQQHLK ALETAARICR 70 80 90 100 110 120 AQISGLFPGS TEISFVPVEI EGGKYDIDIG TAGSITLLLQ CIMPALPFAK EKVELTIKGG 130 140 150 160 170 180 TDVAWSPTID YLQHVTFRAL EQLGYSGSIT LKEHGYYPKG GGKVSAYFKP CRLREFHFLK 190 200 210 220 230 240 EKEDIKGSSH ASNLPAHVPL RQAEAASKRL MEAGYPSLIE TQSFEAFSIG SGITLWIGYI 250 260 270 280 290 300 GGSALGERGL PAEKVGKNAA DEIIPELRSG ASVDTHLADQ LIPYMALAGN SSYTVRELSL 310 320 330 HTTTNIWVTE QFLDVKFRIE KKEGLFEVSV S

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000099 Genomic DNA. Translation: AAZ69933.1.
RefSeq	YP_304513.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q46DV9.
Genome annotation databases
GeneID	3626457.
GenomeReviews	Gene locus Mbar_A0961 in contig CP000099_GR.
KEGG	mba:Mbar_A0961.
NMPDR	fig|269797.3.peg.976.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46DV9.
OMA	EIDGSYG.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0961-MON.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_METBF

Accession

Primary (citable) accession number: Q46DV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)