Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q46DC4 (DAPA_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:Mbar_A1150
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000050213

Regions

Region48 – 492Pyruvate binding By similarity

Sites

Active site1591Schiff-base intermediate with substrate By similarity
Binding site1041Pyruvate By similarity
Site1311Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46DC4 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: FB0904695825A86E

FASTA29130,934
        10         20         30         40         50         60 
MFEGAMPALI TPFTKDDRID REGLQRNIEF VEEGGVAGIV PCGTTGESAT LSALEHEEVI 

        70         80         90        100        110        120 
DIAVECSKVP VIAGTGSNNT GEALQFTKHA ADAGVDGVLL ISPYYNKPNP AGLLTHFKKI 

       130        140        150        160        170        180 
AEAVDVPMVM YNIPSRTGQD MPLEVIVELA KVENIVGIKE ASGNIGKVSQ ILENTIDEDF 

       190        200        210        220        230        240 
VVISGEDNLT LPILSVGGQG VISVAANIVP DRMSRMVNAA LAGDYETARK IHFEIAPLIR 

       250        260        270        280        290 
ALFLETNPIP VKKAAELAGL ASGNLRLPLA PLSETNTQKV ADELRKLGVI E

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000099 Genomic DNA. Translation: AAZ70118.1.
RefSeqYP_304698.1. NC_007355.1.

3D structure databases

HSSPHSSP built from PDB template 2A6N based on UniProtKB P0A6L2.
ProteinModelPortalQ46DC4.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ46DC4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3626283.
GenomeReviewsGene locus Mbar_A1150 in contig CP000099_GR.
KEGGmba:Mbar_A1150.
NMPDRfig|269797.3.peg.1161.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG05039.
HOGENOMHBG358848.
OMACEMEDSN.
PhylomeDBQ46DC4.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycMBAR269797:MBAR_A1150-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_METBF
AccessionPrimary (citable) accession number: Q46DC4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents