Q46D96 (GUAAB_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Mbar_A1178

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW asparagine biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000048376
Regions
	Domain	2 – 184	183	GMPS ATP-PPase
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46D96 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9C4E59729124E46E	FASTA	305	34,093
	10 20 30 40 50 60 MVKPEKFIPK AVEKISKEIK DGRAIIALSG GVDSSVCAEL AHRAIGGRLQ PIYIDTGLMR 70 80 90 100 110 120 KGETERIKHI FSHMNLDIVY AKDRFLAALK GITDPEEKRK AIGETFIRVF EEEARKLEAD 130 140 150 160 170 180 YLIQGTIYPD RIESEGGIKS HHNVGGLPSV MDFKKIVEPI EDLYKDEVRE VAWALKLPDE 190 200 210 220 230 240 ICERMPFPGP GLAVRILGEV TEEKLEVARE ANSIVEEELL DRFCPWQTFA AVIGKGTGVK 250 260 270 280 290 300 GDIRAYGWII AVRAVGSRDG MTAEALELPW DVLKKLESRI TSEIPKVARV VYDITPKPPA TIEFE

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ70146.1.
RefSeq	YP_304726.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46D96.
SMR	Q46D96. Positions 4-305.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46D96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3624525.
GenomeReviews	Gene locus Mbar_A1178 in contig CP000099_GR.
KEGG	mba:Mbar_A1178.
NMPDR	fig|269797.3.peg.1189.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04539.
HOGENOM	HBG391775.
OMA	QGTIAPD.
ProtClustDB	PRK00919.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1178-MONOMER.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001962. Asn_synthase. IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00733. Asn_synthase. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_METBF

Accession

Primary (citable) accession number: Q46D96

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program