Reviewed, UniProtKB/Swiss-Prot Q46D96 (GUAAB_METBF)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit B
    EC=6.3.5.2
Alternative name(s):
    GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Mbar_A1178

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000048376
Regions
	Domain	33 – 185	153	GMP-binding
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46D96-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9C4E59729124E46E	FASTA	305	34,093
	10 20 30 40 50 60 MVKPEKFIPK AVEKISKEIK DGRAIIALSG GVDSSVCAEL AHRAIGGRLQ PIYIDTGLMR 70 80 90 100 110 120 KGETERIKHI FSHMNLDIVY AKDRFLAALK GITDPEEKRK AIGETFIRVF EEEARKLEAD 130 140 150 160 170 180 YLIQGTIYPD RIESEGGIKS HHNVGGLPSV MDFKKIVEPI EDLYKDEVRE VAWALKLPDE 190 200 210 220 230 240 ICERMPFPGP GLAVRILGEV TEEKLEVARE ANSIVEEELL DRFCPWQTFA AVIGKGTGVK 250 260 270 280 290 300 GDIRAYGWII AVRAVGSRDG MTAEALELPW DVLKKLESRI TSEIPKVARV VYDITPKPPA TIEFE

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000099 Genomic DNA. Translation: AAZ70146.1.
RefSeq	YP_304726.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q46D96.
Genome annotation databases
GeneID	3624525.
GenomeReviews	Gene locus Mbar_A1178 in contig CP000099_GR.
KEGG	mba:Mbar_A1178.
NMPDR	fig|269797.3.peg.1189.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46D96.
OMA	GIKSHHN.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1178-MON.
Family and domain databases
HAMAP	MF_00345. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR018317. Queuosine_synth-like. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF06508. ExsB. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUAAB_METBF

Accession

Primary (citable) accession number: Q46D96

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 13, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)