Q46D82 (SURE_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-nucleotidase surE
EC=3.1.3.5

Alternative name(s):
Nucleoside 5'-monophosphate phosphohydrolase

Gene names

Name:	surE
Ordered Locus Names:	Mbar_A1192

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates By similarity. HAMAP MF_00060
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00060.
Sequence similarities	Belongs to the surE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	5'-nucleotidase surE HAMAP MF_00060		PRO_0000235677
Sites
	Metal binding	14	1	Divalent metal cation By similarity
	Metal binding	15	1	Divalent metal cation By similarity
	Metal binding	45	1	Divalent metal cation By similarity
	Metal binding	100	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46D82 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: BF40B5A47F30AA7F	FASTA	267	29,074
	10 20 30 40 50 60 MGKLMAPKIL VTNDDGVYST GLKAAFDSVS DLGEVTISAP AVQQSGVGRS ISIFEPLRIT 70 80 90 100 110 120 KTNAGGIPAY SVGGTPTDAV ILGIFTILKE MPDLVLSGFN IGENISTDTI TTSGTIGGAL 130 140 150 160 170 180 EAASYGVPAI AASMQVLDEG QKFDDPRDYH RERFEAGIKV VNRVAQNVLN YGMPENVDLL 190 200 210 220 230 240 NINIPYHAEE DTPIEITRLA RKIFKTDVEE RRDPRGRPYY WIAGDLIREE EEGTDVHAIM 250 260 QKGHVSITPI SLDSTARIEF SEIEKYL

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ70160.1.
RefSeq	YP_304740.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46D82.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46D82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3624539.
GenomeReviews	Gene locus Mbar_A1192 in contig CP000099_GR.
KEGG	mba:Mbar_A1192.
NMPDR	fig|269797.3.peg.1202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05083.
HOGENOM	HBG600532.
OMA	NGFYYVN.
PhylomeDB	Q46D82.
ProtClustDB	PRK00346.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1192-MONOMER.
Family and domain databases
HAMAP	MF_00060. SurE. [Tree]
InterPro	IPR002828. SurE-like_Pase/nucleotidase. [Graphical view]
Gene3D	G3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 1 hit.
KO	K03787.
Pfam	PF01975. SurE. 1 hit. [Graphical view]
SUPFAM	SSF64167. SurE-like_Pase/nucleotidase. 1 hit.
TIGRFAMs	TIGR00087. SurE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SURE_METBF

Accession

Primary (citable) accession number: Q46D82

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program