Q46CB8 (RIFK_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Mbar_A1523

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Riboflavin kinase HAMAP MF_01285		PRO_0000322089
Regions
	Nucleotide binding	99 – 104	6	CDP By similarity
	Nucleotide binding	198 – 201	4	CDP By similarity
	Region	1 – 89	89	Unknown HAMAP MF_01285
	Region	90 – 225	136	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	128	1	Magnesium By similarity
	Metal binding	130	1	Magnesium By similarity
	Binding site	185	1	FMN By similarity
	Binding site	193	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46CB8 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 00D87BC1C6D98C00	FASTA	225	25,167
	10 20 30 40 50 60 MPDIKYLKKL ALIGAINKII KVSSSEFQKH TGASSKTTAR KLKQLEDERL IERKIVPGGQ 70 80 90 100 110 120 LIKMTDKGIE VLKNEYVDYS RIFSPDLDIL ELEGKVLKGL GEGQYYVNIP GYRKQFEEKL 130 140 150 160 170 180 HFVPFPGTLN VQLSESSSSL RNLLLETPAI RVEGFNDGER TFGGGKCYPV VVGSIEAAVV 190 200 210 220 VPERTHYPSD LIEIIAPIKL RDALKLKDGD RVVVQLKKQG TENQK

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ70474.1.
RefSeq	YP_305054.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46CB8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46CB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3626924.
GenomeReviews	Gene locus Mbar_A1523 in contig CP000099_GR.
KEGG	mba:Mbar_A1523.
NMPDR	fig|269797.3.peg.1809.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	PRK14165.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1523-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_METBF

Accession

Primary (citable) accession number: Q46CB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program