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Protein

Quinolinate synthase A

Gene

nadA

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241IminoaspartateUniRule annotation
Binding sitei41 – 411Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei112 – 1121IminoaspartateUniRule annotation
Binding sitei129 – 1291IminoaspartateUniRule annotation
Binding sitei197 – 1971IminoaspartateUniRule annotation
Binding sitei214 – 2141IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-1908-MONOMER.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:Mbar_A1865
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Quinolinate synthase APRO_1000061157Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A1865.

Structurei

3D structure databases

ProteinModelPortaliQ46BD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46BD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQAELIERI KELKIKRNAV ILAHYYSRPE VQDIADFVGD SLGLSQEAVR
60 70 80 90 100
QTADVIVFCG VHFMGESAAI LCPDKTVLLP EIDATCPMAD MVDIEGLRRE
110 120 130 140 150
KEKHPNAVVV CYVNSSAAIK AESYICCTSA NAVEVVNSLE AEKVIFVPDK
160 170 180 190 200
NLAAYVESRT DKKIIPWEGH CPTHHQILRE DVLKMKEKHP EAKFIAHPEC
210 220 230 240 250
RPEVLELADH IASTRGMIMY AKNSPAKEFI IGTECGLLHG LHKAAPEKKY
260 270 280 290 300
YCVSEFACCP SMKMVNLEKV LVSLEKVQHV VTVPYNVRTR AKEALDRMLA

VKIR
Length:304
Mass (Da):34,054
Last modified:September 13, 2005 - v1
Checksum:i83E835BB76EABEC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ70805.1.
RefSeqiWP_011306851.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ70805; AAZ70805; Mbar_A1865.
GeneIDi3624567.
KEGGimba:Mbar_A1865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ70805.1.
RefSeqiWP_011306851.1. NC_007355.1.

3D structure databases

ProteinModelPortaliQ46BD7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A1865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ70805; AAZ70805; Mbar_A1865.
GeneIDi3624567.
KEGGimba:Mbar_A1865.

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciMBAR269797:GHUW-1908-MONOMER.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiNADA_METBF
AccessioniPrimary (citable) accession number: Q46BD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 13, 2005
Last modified: December 9, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.