Reviewed, UniProtKB/Swiss-Prot Q46BD6 (ASPD_METBF)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	Mbar_A1866

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H(2)O + NAD(P)(+) = oxaloacetate + NH(3) + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000067303
Sites
	Active site	222	1	By similarity
	Binding site	124	1	NAD; via amide nitrogen By similarity
	Binding site	192	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46BD6-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 868F364A30900982	FASTA	271	28,823
	10 20 30 40 50 60 MLKIGVIGCG FIGGQICRAI DKGVINAELY ALSDSSESKV LELTTCLKRY SPASMTIEEL 70 80 90 100 110 120 LQNVDFVIES ASQKAVRLIV PQALEAGRDV MVMSVGALAD EELRKRLFRL AEQNNCKLYF 130 140 150 160 170 180 PSGAVAGIDG INSASAAEIL SVTLTTRKPP MGLAGAPHVE ALGIELETIE KETLLFEGPA 190 200 210 220 230 240 SEAVKAFPAN VNVAATISLA GIGFERTKVR VIADPTLSRN VHEITVEGEF GKLSTRVENL 250 260 270 PSPENPKTSY LAALSAISTL KKILSPVQIG T

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000099 Genomic DNA. Translation: AAZ70806.1.
RefSeq	YP_305386.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3624568.
GenomeReviews	Gene locus Mbar_A1866 in contig CP000099_GR.
KEGG	mba:Mbar_A1866.
NMPDR	fig|269797.3.peg.2965.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q46BD6.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1866-MON.
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DHase_NAD-bd. IPR002811. Asp_DHase. IPR011182. Asp_DHase_NAD_syn. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_METBF

Accession

Primary (citable) accession number: Q46BD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)