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Q46BD6 (ASPD_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable L-aspartate dehydrogenase
EC=1.4.1.21
Gene names
Name:nadX
Ordered Locus Names:Mbar_A1866
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265

Catalytic activity

L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265

Miscellaneous

The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265

Sequence similarities

Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
   Biological processPyridine nucleotide biosynthesis
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processNAD biosynthetic process

Inferred from electronic annotation. Source: InterPro

NADP catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionNADP binding

Inferred from electronic annotation. Source: InterPro

aspartate dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271Probable L-aspartate dehydrogenase HAMAP MF_01265
PRO_1000067303

Sites

Active site2221 By similarity
Binding site1241NAD; via amide nitrogen By similarity
Binding site1921NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46BD6 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 868F364A30900982

FASTA27128,823
        10         20         30         40         50         60 
MLKIGVIGCG FIGGQICRAI DKGVINAELY ALSDSSESKV LELTTCLKRY SPASMTIEEL 

        70         80         90        100        110        120 
LQNVDFVIES ASQKAVRLIV PQALEAGRDV MVMSVGALAD EELRKRLFRL AEQNNCKLYF 

       130        140        150        160        170        180 
PSGAVAGIDG INSASAAEIL SVTLTTRKPP MGLAGAPHVE ALGIELETIE KETLLFEGPA 

       190        200        210        220        230        240 
SEAVKAFPAN VNVAATISLA GIGFERTKVR VIADPTLSRN VHEITVEGEF GKLSTRVENL 

       250        260        270 
PSPENPKTSY LAALSAISTL KKILSPVQIG T

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000099 Genomic DNA. Translation: AAZ70806.1.
RefSeqYP_305386.1. NC_007355.1.

3D structure databases

HSSPHSSP built from PDB template 1J5P based on UniProtKB Q9X1X6.
ProteinModelPortalQ46BD6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ46BD6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3624568.
GenomeReviewsGene locus Mbar_A1866 in contig CP000099_GR.
KEGGmba:Mbar_A1866.
NMPDRfig|269797.3.peg.2965.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG05209.
HOGENOMHBG649642.
OMAECAGHSA.
ProtClustDBPRK13304.

Enzyme and pathway databases

BioCycMBAR269797:MBAR_A1866-MONOMER.

Family and domain databases

HAMAPMF_01265. NadX.
[Tree]
InterProIPR005106. Asp/hSer_DH_NAD-bd.
IPR002811. Asp_DH.
IPR011182. Asp_DH_NAD_syn.
IPR020626. Asp_DH_NAD_syn_prok.
IPR022487. Asp_DH_NAD_synth_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK06989.
PfamPF01958. DUF108. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFPIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMsTIGR03855. NAD_NadX. 1 hit.
ProtoNetSearch...

Entry information

Entry nameASPD_METBF
AccessionPrimary (citable) accession number: Q46BD6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 13, 2005
Last modified: November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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