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Q46BA3 (DNLI2_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 2

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 2
Gene names
Name:lig2
Ordered Locus Names:Mbar_A1899
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 568568DNA ligase 2 HAMAP-Rule MF_00407
PRO_1000049868

Sites

Active site2561N6-AMP-lysine intermediate By similarity
Binding site2541ATP By similarity
Binding site2611ATP By similarity
Binding site2761ATP By similarity
Binding site3061ATP By similarity
Binding site3461ATP By similarity
Binding site4251ATP By similarity
Binding site4311ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46BA3 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: CF22D6D1E1A08B95

FASTA56863,176
        10         20         30         40         50         60 
MTSFREFAET CQAIEKISST IDTTSRVADL LKKVDVEELP IATHFIMSEV FPAWSGEQLG 

        70         80         90        100        110        120 
IGTSLLYVSL SKASGMPVKS IESLIRTTGD IGDTALLILK EKRKNQVTFS SFFEEQPELS 

       130        140        150        160        170        180 
ITEVYNRFKI ASEASGKGSQ DIKIKNLQFL FTSSTPREAK YISRLALEEL RIGVGEGVVR 

       190        200        210        220        230        240 
DAIAKAFSVP VDVVEHAFMV TNDLGIVAAT AKDGGVEALE SLGIEINRPI KMMLSQISPD 

       250        260        270        280        290        300 
IVADIKEMKE AAIEWKFDGA RVQVHKAGDS VMLFSRKLEN VTNSLPDLVE IIRKHVKAES 

       310        320        330        340        350        360 
AILDGEAVAV DENGRPRAFQ EILKRFRRKY DVEEKALGIP IQLNLFDIMY LNGKTLIDLP 

       370        380        390        400        410        420 
LIERRKALES CVESSVEDSK SISVDKQVIT GDLDLIEKIY KEALDAGHEG VMVKNPNSFY 

       430        440        450        460        470        480 
SPGKRGKNWL KKKPLMETLD LVVVGAEWGF GRRANLLGSY TVACYDPKTL RFLQVGKVGT 

       490        500        510        520        530        540 
GLTDDQLKEL TEILSGLMEG GEAGGIFAIR PKIVLEIAFE EIQKSPNYDS GFALRFPRFI 

       550        560 
RIRDDKDPEE ADTIQRIGKV YSQQLKRL 

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ70839.1.
RefSeqYP_305419.1. NC_007355.1.

3D structure databases

ProteinModelPortalQ46BA3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269797.Mbar_A1899.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ70839; AAZ70839; Mbar_A1899.
GeneID3624300.
KEGGmba:Mbar_A1899.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAGRPRPFQ.
ProtClustDBCLSK568925.

Enzyme and pathway databases

BioCycMBAR269797:GHUW-1942-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI2_METBF
AccessionPrimary (citable) accession number: Q46BA3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families