Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q46BA3

- DNLI2_METBF

UniProt

Q46BA3 - DNLI2_METBF

Protein

DNA ligase 2

Gene

lig2

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

    Cofactori

    Divalent metal cations.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei254 – 2541ATPUniRule annotation
    Active sitei256 – 2561N6-AMP-lysine intermediateUniRule annotation
    Binding sitei261 – 2611ATPUniRule annotation
    Binding sitei276 – 2761ATPUniRule annotation
    Binding sitei306 – 3061ATPUniRule annotation
    Binding sitei346 – 3461ATPUniRule annotation
    Binding sitei425 – 4251ATPUniRule annotation
    Binding sitei431 – 4311ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. DNA ligation involved in DNA repair Source: InterPro
    4. DNA recombination Source: UniProtKB-HAMAP
    5. DNA replication Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-1942-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase 2UniRule annotation (EC:6.5.1.1UniRule annotation)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP] 2UniRule annotation
    Gene namesi
    Name:lig2UniRule annotation
    Ordered Locus Names:Mbar_A1899
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 568568DNA ligase 2PRO_1000049868Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi269797.Mbar_A1899.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46BA3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1793.
    HOGENOMiHOG000036008.
    KOiK10747.
    OMAiARVQVHK.

    Family and domain databases

    Gene3Di1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPiMF_00407. DNA_ligase.
    InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46BA3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSFREFAET CQAIEKISST IDTTSRVADL LKKVDVEELP IATHFIMSEV    50
    FPAWSGEQLG IGTSLLYVSL SKASGMPVKS IESLIRTTGD IGDTALLILK 100
    EKRKNQVTFS SFFEEQPELS ITEVYNRFKI ASEASGKGSQ DIKIKNLQFL 150
    FTSSTPREAK YISRLALEEL RIGVGEGVVR DAIAKAFSVP VDVVEHAFMV 200
    TNDLGIVAAT AKDGGVEALE SLGIEINRPI KMMLSQISPD IVADIKEMKE 250
    AAIEWKFDGA RVQVHKAGDS VMLFSRKLEN VTNSLPDLVE IIRKHVKAES 300
    AILDGEAVAV DENGRPRAFQ EILKRFRRKY DVEEKALGIP IQLNLFDIMY 350
    LNGKTLIDLP LIERRKALES CVESSVEDSK SISVDKQVIT GDLDLIEKIY 400
    KEALDAGHEG VMVKNPNSFY SPGKRGKNWL KKKPLMETLD LVVVGAEWGF 450
    GRRANLLGSY TVACYDPKTL RFLQVGKVGT GLTDDQLKEL TEILSGLMEG 500
    GEAGGIFAIR PKIVLEIAFE EIQKSPNYDS GFALRFPRFI RIRDDKDPEE 550
    ADTIQRIGKV YSQQLKRL 568
    Length:568
    Mass (Da):63,176
    Last modified:September 13, 2005 - v1
    Checksum:iCF22D6D1E1A08B95
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ70839.1.
    RefSeqiWP_011306885.1. NC_007355.1.
    YP_305419.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ70839; AAZ70839; Mbar_A1899.
    GeneIDi3624300.
    KEGGimba:Mbar_A1899.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ70839.1 .
    RefSeqi WP_011306885.1. NC_007355.1.
    YP_305419.1. NC_007355.1.

    3D structure databases

    ProteinModelPortali Q46BA3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A1899.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ70839 ; AAZ70839 ; Mbar_A1899 .
    GeneIDi 3624300.
    KEGGi mba:Mbar_A1899.

    Phylogenomic databases

    eggNOGi COG1793.
    HOGENOMi HOG000036008.
    KOi K10747.
    OMAi ARVQVHK.

    Enzyme and pathway databases

    BioCyci MBAR269797:GHUW-1942-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPi MF_00407. DNA_ligase.
    InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.

    Entry informationi

    Entry nameiDNLI2_METBF
    AccessioniPrimary (citable) accession number: Q46BA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3