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Protein

DNA ligase 2

Gene

lig2

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei254ATPUniRule annotation1
Active sitei256N6-AMP-lysine intermediateUniRule annotation1
Binding sitei261ATPUniRule annotation1
Binding sitei276ATPUniRule annotation1
Binding sitei306ATPUniRule annotation1
Binding sitei346ATPUniRule annotation1
Binding sitei425ATPUniRule annotation1
Binding sitei431ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 2UniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 2UniRule annotation
Gene namesi
Name:lig2UniRule annotation
Ordered Locus Names:Mbar_A1899
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000498681 – 568DNA ligase 2Add BLAST568

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A1899

Structurei

3D structure databases

ProteinModelPortaliQ46BA3
SMRiQ46BA3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347 Archaea
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWLFEESY
OrthoDBiPOG093Z03L0

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

Q46BA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSFREFAET CQAIEKISST IDTTSRVADL LKKVDVEELP IATHFIMSEV
60 70 80 90 100
FPAWSGEQLG IGTSLLYVSL SKASGMPVKS IESLIRTTGD IGDTALLILK
110 120 130 140 150
EKRKNQVTFS SFFEEQPELS ITEVYNRFKI ASEASGKGSQ DIKIKNLQFL
160 170 180 190 200
FTSSTPREAK YISRLALEEL RIGVGEGVVR DAIAKAFSVP VDVVEHAFMV
210 220 230 240 250
TNDLGIVAAT AKDGGVEALE SLGIEINRPI KMMLSQISPD IVADIKEMKE
260 270 280 290 300
AAIEWKFDGA RVQVHKAGDS VMLFSRKLEN VTNSLPDLVE IIRKHVKAES
310 320 330 340 350
AILDGEAVAV DENGRPRAFQ EILKRFRRKY DVEEKALGIP IQLNLFDIMY
360 370 380 390 400
LNGKTLIDLP LIERRKALES CVESSVEDSK SISVDKQVIT GDLDLIEKIY
410 420 430 440 450
KEALDAGHEG VMVKNPNSFY SPGKRGKNWL KKKPLMETLD LVVVGAEWGF
460 470 480 490 500
GRRANLLGSY TVACYDPKTL RFLQVGKVGT GLTDDQLKEL TEILSGLMEG
510 520 530 540 550
GEAGGIFAIR PKIVLEIAFE EIQKSPNYDS GFALRFPRFI RIRDDKDPEE
560
ADTIQRIGKV YSQQLKRL
Length:568
Mass (Da):63,176
Last modified:September 13, 2005 - v1
Checksum:iCF22D6D1E1A08B95
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA Translation: AAZ70839.1
RefSeqiWP_011306885.1, NC_007355.1

Genome annotation databases

EnsemblBacteriaiAAZ70839; AAZ70839; Mbar_A1899
GeneIDi3624300
KEGGimba:Mbar_A1899

Entry informationi

Entry nameiDNLI2_METBF
AccessioniPrimary (citable) accession number: Q46BA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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