Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q46AW0

- SYL_METBF

UniProt

Q46AW0 - SYL_METBF

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-2093-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Mbar_A2048
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 966966Leucine--tRNA ligasePRO_1000009370Add
BLAST

Proteomic databases

PRIDEiQ46AW0.

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A2048.

Structurei

3D structure databases

ProteinModelPortaliQ46AW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiHAPFDYL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46AW0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQDYKPHEI EKKWQKKWDE SQIFQAEPDK REKFFITIPY PYLNGNLHAG
60 70 80 90 100
HTRTFTIGDV VARHKRMLGY NVLYPMGFHV TGTPIVGLAE LIANRDPQTM
110 120 130 140 150
DVYERLHGIP GDILPTLDTP EKIVDYFKRE SEKAMRNIGY SIDWRRKFTT
160 170 180 190 200
TDPTYKKFIE WQYIRLGEKG LIVKGSHPVK WCPNDNNPVE DHDILYGEEA
210 220 230 240 250
TIVEYTLIKF RYKDLVLPCA TLRPETTYGV TNLWVNPDVT YVKAKVTLDG
260 270 280 290 300
NEEFWVVSKE AFRKLTFTDR TVEYIEDVPA KSIIGIKLTN PVTDDEVISL
310 320 330 340 350
PASFVRPENG SGIVMSVPAH APFDYLALRD LYDVDLSEYG ITEDLRKIKL
360 370 380 390 400
ISLIKVPEFG EFPAKEIVES MGITSQKDPK AEEATKIVYR REFHGGVLKE
410 420 430 440 450
ITGKYEGQAV SKIKDILTRD FISSNTGETF YEFSEPVVCR CGTPCVVNMV
460 470 480 490 500
KGQWFLNYSN PEWKAKVYKC LAQMRIVPEE YRVEFENKID WLKDKACARR
510 520 530 540 550
KGLGTHLPFD KEWLIESLGD STIYMSYYII ARFIENGELK IENLTLSFFD
560 570 580 590 600
YVLLGKGDSA AASADTGLSP ELLEEIRRHF NYWYPVDLRS SGKDLVPNHL
610 620 630 640 650
LFFLFHHVAL FEEDKWPKAL AVNGFVSLEG QKMSKSKGPI LTMESAVSKY
660 670 680 690 700
GADITRMYIL STAEQTQDAD WQRTGIESAR RQVDRFYSFA KGVIESGKRA
710 720 730 740 750
DLSTELKQID RWILSRIQNY IRDTNSALYS IQTREAIQNS FFLLQNDVKW
760 770 780 790 800
YQRRGGETLL YYVLDNWVRL MAPFTPHLCE EIWEAMGHKD PVSLAQYPLY
810 820 830 840 850
NEGLIDDGAE LAEEMIKGTL EDVEEIIRVT KMTPQKVHLY TAPTWKAEAI
860 870 880 890 900
RCACEMQLEC SLEVGNLIKK LMANPDLKRF GKEIPKFVQK IVPEFKSGSS
910 920 930 940 950
DRYEILTGPD IDEQALLKES ISFLEKEIGC SVEVHSADSP AFDPEKKARF
960
AEPLRPAIYI EGKKKE
Length:966
Mass (Da):111,095
Last modified:September 13, 2005 - v1
Checksum:iB9AD29D5E713058E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ70982.1.
RefSeqiYP_305562.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ70982; AAZ70982; Mbar_A2048.
GeneIDi3625563.
KEGGimba:Mbar_A2048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ70982.1 .
RefSeqi YP_305562.1. NC_007355.1.

3D structure databases

ProteinModelPortali Q46AW0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269797.Mbar_A2048.

Proteomic databases

PRIDEi Q46AW0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ70982 ; AAZ70982 ; Mbar_A2048 .
GeneIDi 3625563.
KEGGi mba:Mbar_A2048.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000216620.
KOi K01869.
OMAi HAPFDYL.

Enzyme and pathway databases

BioCyci MBAR269797:GHUW-2093-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_A. Leu_tRNA_synth_A.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiSYL_METBF
AccessioniPrimary (citable) accession number: Q46AW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3