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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei635 – 6351ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-2093-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Mbar_A2048
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 966966Leucine--tRNA ligasePRO_1000009370Add
BLAST

Proteomic databases

PRIDEiQ46AW0.

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A2048.

Structurei

3D structure databases

ProteinModelPortaliQ46AW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi632 – 6365"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiTREFHTT.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46AW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQDYKPHEI EKKWQKKWDE SQIFQAEPDK REKFFITIPY PYLNGNLHAG
60 70 80 90 100
HTRTFTIGDV VARHKRMLGY NVLYPMGFHV TGTPIVGLAE LIANRDPQTM
110 120 130 140 150
DVYERLHGIP GDILPTLDTP EKIVDYFKRE SEKAMRNIGY SIDWRRKFTT
160 170 180 190 200
TDPTYKKFIE WQYIRLGEKG LIVKGSHPVK WCPNDNNPVE DHDILYGEEA
210 220 230 240 250
TIVEYTLIKF RYKDLVLPCA TLRPETTYGV TNLWVNPDVT YVKAKVTLDG
260 270 280 290 300
NEEFWVVSKE AFRKLTFTDR TVEYIEDVPA KSIIGIKLTN PVTDDEVISL
310 320 330 340 350
PASFVRPENG SGIVMSVPAH APFDYLALRD LYDVDLSEYG ITEDLRKIKL
360 370 380 390 400
ISLIKVPEFG EFPAKEIVES MGITSQKDPK AEEATKIVYR REFHGGVLKE
410 420 430 440 450
ITGKYEGQAV SKIKDILTRD FISSNTGETF YEFSEPVVCR CGTPCVVNMV
460 470 480 490 500
KGQWFLNYSN PEWKAKVYKC LAQMRIVPEE YRVEFENKID WLKDKACARR
510 520 530 540 550
KGLGTHLPFD KEWLIESLGD STIYMSYYII ARFIENGELK IENLTLSFFD
560 570 580 590 600
YVLLGKGDSA AASADTGLSP ELLEEIRRHF NYWYPVDLRS SGKDLVPNHL
610 620 630 640 650
LFFLFHHVAL FEEDKWPKAL AVNGFVSLEG QKMSKSKGPI LTMESAVSKY
660 670 680 690 700
GADITRMYIL STAEQTQDAD WQRTGIESAR RQVDRFYSFA KGVIESGKRA
710 720 730 740 750
DLSTELKQID RWILSRIQNY IRDTNSALYS IQTREAIQNS FFLLQNDVKW
760 770 780 790 800
YQRRGGETLL YYVLDNWVRL MAPFTPHLCE EIWEAMGHKD PVSLAQYPLY
810 820 830 840 850
NEGLIDDGAE LAEEMIKGTL EDVEEIIRVT KMTPQKVHLY TAPTWKAEAI
860 870 880 890 900
RCACEMQLEC SLEVGNLIKK LMANPDLKRF GKEIPKFVQK IVPEFKSGSS
910 920 930 940 950
DRYEILTGPD IDEQALLKES ISFLEKEIGC SVEVHSADSP AFDPEKKARF
960
AEPLRPAIYI EGKKKE
Length:966
Mass (Da):111,095
Last modified:September 13, 2005 - v1
Checksum:iB9AD29D5E713058E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ70982.1.
RefSeqiYP_305562.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ70982; AAZ70982; Mbar_A2048.
GeneIDi3625563.
KEGGimba:Mbar_A2048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ70982.1.
RefSeqiYP_305562.1. NC_007355.1.

3D structure databases

ProteinModelPortaliQ46AW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A2048.

Proteomic databases

PRIDEiQ46AW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ70982; AAZ70982; Mbar_A2048.
GeneIDi3625563.
KEGGimba:Mbar_A2048.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiTREFHTT.

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-2093-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiSYL_METBF
AccessioniPrimary (citable) accession number: Q46AW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: April 1, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.