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Q46AW0 (SYL_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mbar_A2048
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length966 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 966966Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009370

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46AW0 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: B9AD29D5E713058E

FASTA966111,095
        10         20         30         40         50         60 
MEQDYKPHEI EKKWQKKWDE SQIFQAEPDK REKFFITIPY PYLNGNLHAG HTRTFTIGDV 

        70         80         90        100        110        120 
VARHKRMLGY NVLYPMGFHV TGTPIVGLAE LIANRDPQTM DVYERLHGIP GDILPTLDTP 

       130        140        150        160        170        180 
EKIVDYFKRE SEKAMRNIGY SIDWRRKFTT TDPTYKKFIE WQYIRLGEKG LIVKGSHPVK 

       190        200        210        220        230        240 
WCPNDNNPVE DHDILYGEEA TIVEYTLIKF RYKDLVLPCA TLRPETTYGV TNLWVNPDVT 

       250        260        270        280        290        300 
YVKAKVTLDG NEEFWVVSKE AFRKLTFTDR TVEYIEDVPA KSIIGIKLTN PVTDDEVISL 

       310        320        330        340        350        360 
PASFVRPENG SGIVMSVPAH APFDYLALRD LYDVDLSEYG ITEDLRKIKL ISLIKVPEFG 

       370        380        390        400        410        420 
EFPAKEIVES MGITSQKDPK AEEATKIVYR REFHGGVLKE ITGKYEGQAV SKIKDILTRD 

       430        440        450        460        470        480 
FISSNTGETF YEFSEPVVCR CGTPCVVNMV KGQWFLNYSN PEWKAKVYKC LAQMRIVPEE 

       490        500        510        520        530        540 
YRVEFENKID WLKDKACARR KGLGTHLPFD KEWLIESLGD STIYMSYYII ARFIENGELK 

       550        560        570        580        590        600 
IENLTLSFFD YVLLGKGDSA AASADTGLSP ELLEEIRRHF NYWYPVDLRS SGKDLVPNHL 

       610        620        630        640        650        660 
LFFLFHHVAL FEEDKWPKAL AVNGFVSLEG QKMSKSKGPI LTMESAVSKY GADITRMYIL 

       670        680        690        700        710        720 
STAEQTQDAD WQRTGIESAR RQVDRFYSFA KGVIESGKRA DLSTELKQID RWILSRIQNY 

       730        740        750        760        770        780 
IRDTNSALYS IQTREAIQNS FFLLQNDVKW YQRRGGETLL YYVLDNWVRL MAPFTPHLCE 

       790        800        810        820        830        840 
EIWEAMGHKD PVSLAQYPLY NEGLIDDGAE LAEEMIKGTL EDVEEIIRVT KMTPQKVHLY 

       850        860        870        880        890        900 
TAPTWKAEAI RCACEMQLEC SLEVGNLIKK LMANPDLKRF GKEIPKFVQK IVPEFKSGSS 

       910        920        930        940        950        960 
DRYEILTGPD IDEQALLKES ISFLEKEIGC SVEVHSADSP AFDPEKKARF AEPLRPAIYI 


EGKKKE 

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References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ70982.1.
RefSeqYP_305562.1. NC_007355.1.

3D structure databases

ProteinModelPortalQ46AW0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269797.Mbar_A2048.

Proteomic databases

PRIDEQ46AW0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ70982; AAZ70982; Mbar_A2048.
GeneID3625563.
KEGGmba:Mbar_A2048.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAHAPFDYL.
ProtClustDBPRK12300.

Enzyme and pathway databases

BioCycMBAR269797:GHUW-2093-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METBF
AccessionPrimary (citable) accession number: Q46AW0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 13, 2005
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries