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Reviewed, UniProtKB/Swiss-Prot Q46AH5 (G3P1_METBF)

Last modified February 9, 2010. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 1
      Short name=GAPDH 1
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase 1
Gene names
Name: gap1
Ordered Locus Names: Mbar_A2189
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559

Subunit structure

Homotetramer By similarity. HAMAP MF_00559

Subcellular location

Cytoplasm By similarity HAMAP MF_00559.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Glyceraldehyde-3-phosphate dehydrogenase 1 HAMAP MF_00559
PRO_0000232390

Regions

Nucleotide binding13 – 142NAD By similarity
Region140 – 1423Glyceraldehyde 3-phosphate binding By similarity
Region195 – 1962Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1411Nucleophile By similarity
Binding site1111NAD; via amide nitrogen By similarity
Binding site1691NAD By similarity
Binding site3001NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46AH5-1 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: 0C51D7380992EA43

FASTA33836,958
        10         20         30         40         50         60 
MVKAKIAVNG YGTIGKRVAD AVRAQDDMEV IGISKTKPNY EAAVAHQIGY DIYAPAANLE 

        70         80         90        100        110        120 
AFEKAGMPAA GTIEEMVEKA DLVVDCTPGG IGEKNKPMYE KAGVKAIWQG GEDHSLAGFS 

       130        140        150        160        170        180 
FNAICNYEQA VGRDLVRVVS CNTTALCRAI YPIDKELGVK KARVVLARRA TDPNDVKKGP 

       190        200        210        220        230        240 
INAIVPDPIK LPSHHGPDVK SVLPQINITS AALKIPTTLM HVHTVNMEVE KDCTVDDVKN 

       250        260        270        280        290        300 
IFGSQPRIRF VGQGMSSTAE IMEFARDMKR PRNDMWENCI WPESITVGDK ELYFFQAVHQ 

       310        320        330 
ESIVVPENVD AIRAMMEFES DGAKSIEKTN KAIGLSKK 

« Hide

References

[1]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000099 Genomic DNA. Translation: AAZ71117.1.
RefSeqYP_305697.1.

3D structure databases

SMRQ46AH5. Positions 5-335.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ46AH5.

Genome annotation databases

GeneID3626065.
GenomeReviewsGene locus Mbar_A2189 in contig CP000099_GR.
KEGGmba:Mbar_A2189.
NMPDRfig|269797.3.peg.1766.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG04694.
HOGENOMHBG392099.

Enzyme and pathway databases

BioCycMBAR269797:MBAR_A2189-MONOMER.

Family and domain databases

HAMAPMF_00559. G3P_dehdrog_arch.
[Tree]
InterProIPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020831. GlycerAld_3-P_DH_family.
IPR020828. GlycerAld_3-P_DH_NAD(P)_bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3P1_METBF
AccessionPrimary (citable) accession number: Q46AH5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents