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Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

gap1

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gap1), Glyceraldehyde-3-phosphate dehydrogenase 2 (gap2)
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (gpmI1), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (gpmI2), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (Mbar_A0175)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111NAD; via amide nitrogenUniRule annotation1
Active sitei141NucleophileUniRule annotation1
Binding sitei169NADUniRule annotation1
Binding sitei300NAD; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 14NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 1UniRule annotation (EC:1.2.1.59UniRule annotation)
Short name:
GAPDH 1UniRule annotation
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase 1UniRule annotation
Gene namesi
Name:gap1UniRule annotation
Ordered Locus Names:Mbar_A2189
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002323901 – 338Glyceraldehyde-3-phosphate dehydrogenase 1Add BLAST338

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi269797.Mbar_A2189.

Structurei

3D structure databases

ProteinModelPortaliQ46AH5.
SMRiQ46AH5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 142Glyceraldehyde 3-phosphate bindingUniRule annotation3
Regioni195 – 196Glyceraldehyde 3-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
HOGENOMiHOG000223361.
KOiK00150.
OMAiNAIVPNP.
OrthoDBiPOG093Z05Z6.

Family and domain databases

HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiView protein in InterPro
IPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR10836. PTHR10836. 1 hit.
PTHR10836:SF76. PTHR10836:SF76. 1 hit.
PfamiView protein in Pfam
PF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46AH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKAKIAVNG YGTIGKRVAD AVRAQDDMEV IGISKTKPNY EAAVAHQIGY
60 70 80 90 100
DIYAPAANLE AFEKAGMPAA GTIEEMVEKA DLVVDCTPGG IGEKNKPMYE
110 120 130 140 150
KAGVKAIWQG GEDHSLAGFS FNAICNYEQA VGRDLVRVVS CNTTALCRAI
160 170 180 190 200
YPIDKELGVK KARVVLARRA TDPNDVKKGP INAIVPDPIK LPSHHGPDVK
210 220 230 240 250
SVLPQINITS AALKIPTTLM HVHTVNMEVE KDCTVDDVKN IFGSQPRIRF
260 270 280 290 300
VGQGMSSTAE IMEFARDMKR PRNDMWENCI WPESITVGDK ELYFFQAVHQ
310 320 330
ESIVVPENVD AIRAMMEFES DGAKSIEKTN KAIGLSKK
Length:338
Mass (Da):36,958
Last modified:September 13, 2005 - v1
Checksum:i0C51D7380992EA43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ71117.1.
RefSeqiWP_011307163.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ71117; AAZ71117; Mbar_A2189.
GeneIDi3626065.
KEGGimba:Mbar_A2189.

Similar proteinsi

Entry informationi

Entry nameiG3P1_METBF
AccessioniPrimary (citable) accession number: Q46AH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: November 22, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families