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Q46AH3

- NADK_METBF

UniProt

Q46AH3 - NADK_METBF

Protein

NAD kinase

Gene

nadK

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.UniRule annotation

    Cofactori

    Divalent metal ions.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei68 – 681Proton acceptorUniRule annotation
    Binding sitei73 – 731NADUniRule annotation
    Binding sitei147 – 1471NADUniRule annotation
    Binding sitei164 – 1641NADUniRule annotation
    Binding sitei166 – 1661NADUniRule annotation
    Binding sitei201 – 2011NAD; via carbonyl oxygenUniRule annotation
    Binding sitei236 – 2361NADUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi68 – 692NADUniRule annotation
    Nucleotide bindingi136 – 1372NADUniRule annotation
    Nucleotide bindingi177 – 1826NADUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-HAMAP
    3. NAD+ kinase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. NAD metabolic process Source: InterPro
    2. NADP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-2239-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
    Alternative name(s):
    ATP-dependent NAD kinaseUniRule annotation
    Gene namesi
    Name:nadKUniRule annotation
    Ordered Locus Names:Mbar_A2191
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275NAD kinasePRO_0000229719Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi269797.Mbar_A2191.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46AH3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0061.
    HOGENOMiHOG000227222.
    KOiK00858.
    OMAiGVLWCDG.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q46AH3-1 [UniParc]FASTAAdd to Basket

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    MAIKKIGISA RCDRPEIIGK VREILAHFSS RVQIFVATPT AEVLGMEGTP    50
    VERMREEGVE LIISVGGDGT VLRNIAKMKD PLPILGINMG TLGFLVDVEP 100
    EDALETIEEV LYGFSYSDRM RVDVFLNGEM LETATNEIAI MSAKPAKIIQ 150
    FEVYVGDCLL DSMRADGVVF ATPTGSTAYA MSAGGPIISP RVNAIVVVPV 200
    APFKLSSRPW VIPSDSEITI RLSAPKKEAV IAIDGQKSYR IKLDDVVKLK 250
    KSRFPARFVR ISDTCFYERV QRKLT 275
    Length:275
    Mass (Da):30,223
    Last modified:September 13, 2005 - v1
    Checksum:i3FFB329FA1A00E04
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ71119.1.
    RefSeqiWP_011307165.1. NC_007355.1.
    YP_305699.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ71119; AAZ71119; Mbar_A2191.
    GeneIDi3626067.
    KEGGimba:Mbar_A2191.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000099 Genomic DNA. Translation: AAZ71119.1 .
    RefSeqi WP_011307165.1. NC_007355.1.
    YP_305699.1. NC_007355.1.

    3D structure databases

    ProteinModelPortali Q46AH3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A2191.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ71119 ; AAZ71119 ; Mbar_A2191 .
    GeneIDi 3626067.
    KEGGi mba:Mbar_A2191.

    Phylogenomic databases

    eggNOGi COG0061.
    HOGENOMi HOG000227222.
    KOi K00858.
    OMAi GVLWCDG.

    Enzyme and pathway databases

    BioCyci MBAR269797:GHUW-2239-MONOMER.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.

    Entry informationi

    Entry nameiNADK_METBF
    AccessioniPrimary (citable) accession number: Q46AH3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3