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Q46AH3

- NADK_METBF

UniProt

Q46AH3 - NADK_METBF

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Protein

NAD kinase

Gene

nadK

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

Divalent metal ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681Proton acceptorUniRule annotation
Binding sitei73 – 731NADUniRule annotation
Binding sitei147 – 1471NADUniRule annotation
Binding sitei164 – 1641NADUniRule annotation
Binding sitei166 – 1661NADUniRule annotation
Binding sitei201 – 2011NAD; via carbonyl oxygenUniRule annotation
Binding sitei236 – 2361NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 692NADUniRule annotation
Nucleotide bindingi136 – 1372NADUniRule annotation
Nucleotide bindingi177 – 1826NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-2239-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Mbar_A2191
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275NAD kinasePRO_0000229719Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A2191.

Structurei

3D structure databases

ProteinModelPortaliQ46AH3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiGVLWCDG.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46AH3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIKKIGISA RCDRPEIIGK VREILAHFSS RVQIFVATPT AEVLGMEGTP
60 70 80 90 100
VERMREEGVE LIISVGGDGT VLRNIAKMKD PLPILGINMG TLGFLVDVEP
110 120 130 140 150
EDALETIEEV LYGFSYSDRM RVDVFLNGEM LETATNEIAI MSAKPAKIIQ
160 170 180 190 200
FEVYVGDCLL DSMRADGVVF ATPTGSTAYA MSAGGPIISP RVNAIVVVPV
210 220 230 240 250
APFKLSSRPW VIPSDSEITI RLSAPKKEAV IAIDGQKSYR IKLDDVVKLK
260 270
KSRFPARFVR ISDTCFYERV QRKLT
Length:275
Mass (Da):30,223
Last modified:September 13, 2005 - v1
Checksum:i3FFB329FA1A00E04
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ71119.1.
RefSeqiYP_305699.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ71119; AAZ71119; Mbar_A2191.
GeneIDi3626067.
KEGGimba:Mbar_A2191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000099 Genomic DNA. Translation: AAZ71119.1 .
RefSeqi YP_305699.1. NC_007355.1.

3D structure databases

ProteinModelPortali Q46AH3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269797.Mbar_A2191.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ71119 ; AAZ71119 ; Mbar_A2191 .
GeneIDi 3626067.
KEGGi mba:Mbar_A2191.

Phylogenomic databases

eggNOGi COG0061.
HOGENOMi HOG000227222.
KOi K00858.
OMAi GVLWCDG.

Enzyme and pathway databases

BioCyci MBAR269797:GHUW-2239-MONOMER.

Family and domain databases

Gene3Di 2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPi MF_00361. NAD_kinase.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view ]
PANTHERi PTHR20275. PTHR20275. 1 hit.
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.

Entry informationi

Entry nameiNADK_METBF
AccessioniPrimary (citable) accession number: Q46AH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3