Q46AH3 (PPNK_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	Mbar_A2191

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP MF_00361
Cofactor	Divalent metal ions By similarity. HAMAP MF_00361
Subcellular location	Cytoplasm By similarity HAMAP MF_00361.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361		PRO_0000229719

Sequences

	Sequence		Length	Mass (Da)
	Q46AH3 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 3FFB329FA1A00E04	FASTA	275	30,223
	10 20 30 40 50 60 MAIKKIGISA RCDRPEIIGK VREILAHFSS RVQIFVATPT AEVLGMEGTP VERMREEGVE 70 80 90 100 110 120 LIISVGGDGT VLRNIAKMKD PLPILGINMG TLGFLVDVEP EDALETIEEV LYGFSYSDRM 130 140 150 160 170 180 RVDVFLNGEM LETATNEIAI MSAKPAKIIQ FEVYVGDCLL DSMRADGVVF ATPTGSTAYA 190 200 210 220 230 240 MSAGGPIISP RVNAIVVVPV APFKLSSRPW VIPSDSEITI RLSAPKKEAV IAIDGQKSYR 250 260 270 IKLDDVVKLK KSRFPARFVR ISDTCFYERV QRKLT

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ71119.1.
RefSeq	YP_305699.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46AH3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46AH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3626067.
GenomeReviews	Gene locus Mbar_A2191 in contig CP000099_GR.
KEGG	mba:Mbar_A2191.
NMPDR	fig|269797.3.peg.1768.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04399.
HOGENOM	HBG713904.
OMA	GPVVWPE.
PhylomeDB	Q46AH3.
ProtClustDB	CLSK568985.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A2191-MONOMER.
Family and domain databases
HAMAP	MF_00361. NAD_kinase. [Tree]
InterPro	IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017438. ATP-NAD_kinase_PpnK-typ_a/b. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. polyP/ATP_NADK_prd. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit. G3DSA:3.40.50.10330. ATP-NAD_kinase_PpnK-typ_a/b. 1 hit.
KO	K00858.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_METBF

Accession

Primary (citable) accession number: Q46AH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	September 13, 2005
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program