Q46A48 (Q46A48_METBF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Mbar_A2320

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP] EMBL AAZ71244.1

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 EMBL AAZ71244.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	39 – 46	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	41	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	Q46A48 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 9EB20928316A9CFB	FASTA	265	28,973
	10 20 30 40 50 60 MPDEYQGIDS MNLEIAQNRI IDFIRNETRK AGVNGAVVGI SGGIDSALAA TLTVKALGKD 70 80 90 100 110 120 KVLGIHMPES GLTPSEDSKD AKTLADWLGI EFQTIDISGI ISAFVASVPE SESTDRLSMG 130 140 150 160 170 180 NLKARIRMSL LYFHANRMNR MVIGTGNKTE ILLGYFTKYG DGGVDLEPIG RLYKTEVWEL 190 200 210 220 230 240 SRRLGVPDAL ITKKPSAGLW AGQTDEAELG ISYLKVDEVL KKLEQNEDSE TILNTLGISA 250 260 EQLKSVMNRI EKSEHKRNAP PVPPN

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ71244.1.
RefSeq	YP_305824.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q46A48.
ModBase	Search...
Protein-protein interaction databases
STRING	Q46A48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3625228.
GenomeReviews	Gene locus Mbar_A2320 in contig CP000099_GR.
KEGG	mba:Mbar_A2320.
NMPDR	fig|269797.3.peg.2277.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	YDISARD.
PhylomeDB	Q46A48.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q46A48_METBF

Accession

Primary (citable) accession number: Q46A48

Entry history

Integrated into UniProtKB/TrEMBL:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)