Reviewed, UniProtKB/Swiss-Prot Q469A3 (CAPPA_METBF)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate carboxylase
      Short name=PEPCase
      Short name=PEPC
    EC=4.1.1.31

Gene names

Name:	ppcA
Ordered Locus Names:	Mbar_A2632

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity.
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + CO2. HAMAP MF_01904
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_01904
Sequence similarities	Belongs to the PEPCase type 2 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Tricarboxylic acid cycle
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbon utilization by fixation of carbon dioxide Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Phosphoenolpyruvate carboxylase HAMAP MF_01904		PRO_0000309603

Sequences

	Sequence		Length	Mass (Da)
	Q469A3-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: A08490FAE19B6E42	FASTA	526	58,068
	10 20 30 40 50 60 MSRKTVFPKV MCTQHPDSAS KYIATQEEPG EAIEAAQIFG CDEYMPDYEG KATPYHQNVQ 70 80 90 100 110 120 VVSKFIEETD LIPGKDIIIT PRAPSAVQEN QFRQLMVMMS IAEANYNALE YSDVQAINEF 130 140 150 160 170 180 VHPMTDSVRE IIGAQQHMVD VSELAKKEFG FSMEVPCIIP LIEDAPALLH AKELAENTLF 190 200 210 220 230 240 AWKGHFGTVP DKFRVFLGKS DSALSFGHVA STLSCKYAIN GICELNSELD TQTGIIFGAG 250 260 270 280 290 300 TLPFRGHLDL MNAENFFKEY RGVGTITLQS ALRYSHKKGD AESLVKLAKA RLPETPELFS 310 320 330 340 350 360 AEEKEEIINL IGIFGASYSR IIRQLAPTIN RIADLLPQQR DRLMHKGTGG YSRSAPDISG 370 380 390 400 410 420 LVNLCRTDIG KELEASMPAE DLQLPRAIKF TGALYSIGLP PEFIGTGRAL EEAREKLGEA 430 440 450 460 470 480 ACENLLTKYF PSLAGDLNFA SEYLDLNVAS RFLSSECLKE VSKDLDILHG AFALETSPEP 490 500 510 520 SYRILVEMMQ PDLLQAGSRG NCMDEEVSQL VCSTLTKMGK IRKALG

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000099 Genomic DNA. Translation: AAZ71539.1.
RefSeq	YP_306119.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3625651.
GenomeReviews	Gene locus Mbar_A2632 in contig CP000099_GR.
KEGG	mba:Mbar_A2632.
NMPDR	fig|269797.3.peg.3318.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q469A3.
OMA	Q469A3. RSDPAMN.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A2632-MON.
Family and domain databases
HAMAP	MF_01904. [Tree]
InterPro	IPR007566. PEnol-Pyrv_COase_arc. [Graphical view]
Pfam	PF04477. DUF557. 1 hit. [Graphical view]
PIRSF	PIRSF006677. UCP006677. 1 hit.
TIGRFAMs	TIGR02751. PEPCase_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPPA_METBF

Accession

Primary (citable) accession number: Q469A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)