ID   HPAG_ECOLX              Reviewed;         429 AA.
AC   Q46978;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase;
DE   Includes:
DE     RecName: Full=2-hydroxyhepta-2,4-diene-1,7-dioate isomerase;
DE              Short=HHDD isomerase;
DE              EC=5.3.3.10;
DE     AltName: Full=5-carboxymethyl-2-hydroxymuconate Delta-isomerase;
DE   Includes:
DE     RecName: Full=5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase;
DE              EC=4.1.1.68;
DE     AltName: Full=5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase;
DE              Short=OPET decarboxylase;
GN   Name=hpaG;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W / ATCC 11105 / DSM 1900;
RX   MEDLINE=96125235; PubMed=8550403;
RA   Prieto M.A., Diaz E., Garcia J.L.;
RT   "Molecular characterization of the 4-hydroxyphenylacetate catabolic
RT   pathway of Escherichia coli W: engineering a mobile aromatic
RT   degradative cluster.";
RL   J. Bacteriol. 178:111-120(1996).
CC   -!- FUNCTION: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-
CC       tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-
CC       dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate).
CC   -!- CATALYTIC ACTIVITY: 5-carboxymethyl-2-hydroxymuconate = 5-carboxy-
CC       2-oxohept-3-enedioate.
CC   -!- CATALYTIC ACTIVITY: 5-oxopent-3-ene-1,2,5-tricarboxylate = 2-
CC       oxohept-3-enedioate + CO(2).
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC       degradation; pyruvate and succinate semialdehyde from 4-
CC       hydroxyphenylacetate: step 4/7.
CC   -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC       degradation; pyruvate and succinate semialdehyde from 4-
CC       hydroxyphenylacetate: step 5/7.
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the FAH family.
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DR   EMBL; Z37980; CAA86040.1; -; Genomic_DNA.
DR   SMR; Q46978; 1-429.
DR   BioCyc; MetaCyc:MON-604; -.
DR   BRENDA; 4.1.1.68; 246.
DR   BRENDA; 5.3.3.10; 246.
DR   GO; GO:0008704; F:5-carboxymethyl-2-hydroxymuconate delta-iso...; IEA:EC.
DR   GO; GO:0018800; F:5-oxopent-3-ene-1,2,5-tricarboxylate decarb...; IEA:EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002529; Fumarylacetoacetase_C-like.
DR   InterPro; IPR011234; Fumarylacetoacetase_C-rel.
DR   InterPro; IPR012684; HPA_isomer/decarb_C.
DR   InterPro; IPR012686; HPA_isomer/decarb_N.
DR   Gene3D; G3DSA:3.90.850.10; Fumarylacetoacetase_C-rel; 2.
DR   Pfam; PF01557; FAA_hydrolase; 2.
DR   TIGRFAMs; TIGR02303; HpaG-C-term; 1.
DR   TIGRFAMs; TIGR02305; HpaG-N-term; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Isomerase; Lyase; Metal-binding;
KW   Multifunctional enzyme; Repeat.
FT   CHAIN         1    429       4-hydroxyphenylacetate degradation
FT                                bifunctional isomerase/decarboxylase.
FT                                /FTId=PRO_0000156833.
FT   REPEAT        1    215       Approximate.
FT   REPEAT      216    429       Approximate.
FT   METAL       276    276       Divalent metal cation (By similarity).
FT   METAL       278    278       Divalent metal cation (By similarity).
FT   METAL       307    307       Divalent metal cation (By similarity).
SQ   SEQUENCE   429 AA;  47108 MW;  BC8AC5E6FEB455B1 CRC64;
     MKGTIFAVAL NHRSQLDAWQ EAFQQSPYKA PPKTAVWFIK PRNTVIGCGE PIPFPQGEKV
     LSGATVALIV GKTATKVREE DAAEYIAGYA LANDVSLPEE SFYRPAIKAK CRDGFCPIGE
     TVALSNVDNL TIYTEINGRP ADHWNTADLQ RNAAQLLSAL SEFATLNPGD AILLGTPQAR
     VEIQPGDRVR VLAEGFPPLE NPVVDEREVT TRKSFPTLPH PHGTLFALGL NYADHASELE
     FKPPEEPLVF LKAPNTLTGD NQTSVRPNNI EYMHYEAELV VVIGKQARNV SEADAMDYVA
     GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPMLSTIVPK EAIPDPHNLT LRTFVNGELR
     QQGTTADLIF SVPFLIAYLS EFMTLNPGDM IATGTPKGLS DVVPGDEVVV EVEGVGRLVN
     RIVSEETAK
//