Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q46978 (HPAG_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase

Including the following 2 domains:

  1. 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase
    Short name=HHDD isomerase
    EC=5.3.3.10
    Alternative name(s):
    5-carboxymethyl-2-hydroxymuconate Delta-isomerase
  2. 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase
    EC=4.1.1.68
    Alternative name(s):
    5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
    Short name=OPET decarboxylase
Gene names
Name:hpaG
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate).

Catalytic activity

5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate.

5-oxopent-3-ene-1,2,5-tricarboxylate = 2-oxohept-3-enedioate + CO2.

Cofactor

Magnesium By similarity.

Pathway

Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 4/7.

Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 5/7.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the FAH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4294294-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase
PRO_0000156833

Regions

Repeat1 – 215215Approximate
Repeat216 – 429214Approximate

Sites

Metal binding2761Divalent metal cation By similarity
Metal binding2781Divalent metal cation By similarity
Metal binding3071Divalent metal cation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q46978 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BC8AC5E6FEB455B1

FASTA42947,108
        10         20         30         40         50         60 
MKGTIFAVAL NHRSQLDAWQ EAFQQSPYKA PPKTAVWFIK PRNTVIGCGE PIPFPQGEKV 

        70         80         90        100        110        120 
LSGATVALIV GKTATKVREE DAAEYIAGYA LANDVSLPEE SFYRPAIKAK CRDGFCPIGE 

       130        140        150        160        170        180 
TVALSNVDNL TIYTEINGRP ADHWNTADLQ RNAAQLLSAL SEFATLNPGD AILLGTPQAR 

       190        200        210        220        230        240 
VEIQPGDRVR VLAEGFPPLE NPVVDEREVT TRKSFPTLPH PHGTLFALGL NYADHASELE 

       250        260        270        280        290        300 
FKPPEEPLVF LKAPNTLTGD NQTSVRPNNI EYMHYEAELV VVIGKQARNV SEADAMDYVA 

       310        320        330        340        350        360 
GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPMLSTIVPK EAIPDPHNLT LRTFVNGELR 

       370        380        390        400        410        420 
QQGTTADLIF SVPFLIAYLS EFMTLNPGDM IATGTPKGLS DVVPGDEVVV EVEGVGRLVN 


RIVSEETAK 

« Hide

References

[1]"Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster."
Prieto M.A., Diaz E., Garcia J.L.
J. Bacteriol. 178:111-120(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: W / ATCC 11105 / DSM 1900.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z37980 Genomic DNA. Translation: CAA86040.1.

3D structure databases

ProteinModelPortalQ46978.
SMRQ46978. Positions 1-429.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-604.
UniPathwayUPA00208; UER00419.
UPA00208; UER00420.

Family and domain databases

Gene3D3.90.850.10. 2 hits.
InterProIPR002529. Fumarylacetoacetase_C.
IPR011234. Fumarylacetoacetase_C-rel.
IPR012684. HPA_isomer/decarb_C.
IPR012686. HPA_isomer/decarb_N.
[Graphical view]
PfamPF01557. FAA_hydrolase. 2 hits.
[Graphical view]
SUPFAMSSF56529. SSF56529. 2 hits.
TIGRFAMsTIGR02303. HpaG-C-term. 1 hit.
TIGR02305. HpaG-N-term. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHPAG_ECOLX
AccessionPrimary (citable) accession number: Q46978
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways