Reviewed, UniProtKB/Swiss-Prot Q46978 (HPAG_ECOLX)

Names and origin

Protein names

Recommended name:
    4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase
Including the following 2 domains:
    1- Recommended name:
            2-hydroxyhepta-2,4-diene-1,7-dioate isomerase
                Short name=HHDD isomerase
              EC=5.3.3.10
        Alternative name(s):
            5-carboxymethyl-2-hydroxymuconate Delta-isomerase
    2- Recommended name:
            5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase
              EC=4.1.1.68
        Alternative name(s):
            5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
              Short name=OPET decarboxylase

Gene names

Name:

hpaG

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate).
Catalytic activity	5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate. 5-oxopent-3-ene-1,2,5-tricarboxylate = 2-oxohept-3-enedioate + CO2.
Cofactor	Magnesium By similarity.
Pathway	Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 4/7. Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 5/7.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the FAH family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Domain	Repeat
Ligand	Metal-binding
Molecular function	Isomerase Lyase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-carboxymethyl-2-hydroxymuconate delta-isomerase activity Inferred from electronic annotation. Source: EC 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase		PRO_0000156833
Regions
	Repeat	1 – 215	215	Approximate
	Repeat	216 – 429	214	Approximate
Sites
	Metal binding	276	1	Divalent metal cation By similarity
	Metal binding	278	1	Divalent metal cation By similarity
	Metal binding	307	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46978-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BC8AC5E6FEB455B1	FASTA	429	47,108
	10 20 30 40 50 60 MKGTIFAVAL NHRSQLDAWQ EAFQQSPYKA PPKTAVWFIK PRNTVIGCGE PIPFPQGEKV 70 80 90 100 110 120 LSGATVALIV GKTATKVREE DAAEYIAGYA LANDVSLPEE SFYRPAIKAK CRDGFCPIGE 130 140 150 160 170 180 TVALSNVDNL TIYTEINGRP ADHWNTADLQ RNAAQLLSAL SEFATLNPGD AILLGTPQAR 190 200 210 220 230 240 VEIQPGDRVR VLAEGFPPLE NPVVDEREVT TRKSFPTLPH PHGTLFALGL NYADHASELE 250 260 270 280 290 300 FKPPEEPLVF LKAPNTLTGD NQTSVRPNNI EYMHYEAELV VVIGKQARNV SEADAMDYVA 310 320 330 340 350 360 GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPMLSTIVPK EAIPDPHNLT LRTFVNGELR 370 380 390 400 410 420 QQGTTADLIF SVPFLIAYLS EFMTLNPGDM IATGTPKGLS DVVPGDEVVV EVEGVGRLVN RIVSEETAK

References

[1]

"Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster." Prieto M.A., Diaz E., Garcia J.L. J. Bacteriol. 178:111-120(1996) [PubMed: 8550403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W / ATCC 11105 / DSM 1900.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z37980 Genomic DNA. Translation: CAA86040.1.
3D structure databases
SMR	Q46978. Positions 1-429.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-604.
BRENDA	4.1.1.68. 246. 5.3.3.10. 246.
Family and domain databases
InterPro	IPR002529. Fumarylacetoacetase_C-like. IPR011234. Fumarylacetoacetase_C-rel. IPR012684. HPA_isomer/decarb_C. IPR012686. HPA_isomer/decarb_N. [Graphical view]
Gene3D	G3DSA:3.90.850.10. Fumarylacetoacetase_C-rel. 2 hits.
Pfam	PF01557. FAA_hydrolase. 2 hits. [Graphical view]
TIGRFAMs	TIGR02303. HpaG-C-term. 1 hit. TIGR02305. HpaG-N-term. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPAG_ECOLX

Accession

Primary (citable) accession number: Q46978

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)