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Q46978

- HPAG_ECOLX

UniProt

Q46978 - HPAG_ECOLX

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Protein

4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase

Gene

hpaG

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate).

Catalytic activityi

5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate.
5-oxopent-3-ene-1,2,5-tricarboxylate = 2-oxohept-3-enedioate + CO2.

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi276 – 2761Divalent metal cationBy similarity
Metal bindingi278 – 2781Divalent metal cationBy similarity
Metal bindingi307 – 3071Divalent metal cationBy similarity

GO - Molecular functioni

  1. 5-carboxymethyl-2-hydroxymuconate delta-isomerase activity Source: UniProtKB-EC
  2. 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 4-hydroxyphenylacetate catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-604.
UniPathwayiUPA00208; UER00419.
UPA00208; UER00420.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase
Including the following 2 domains:
2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC:5.3.3.10)
Short name:
HHDD isomerase
Alternative name(s):
5-carboxymethyl-2-hydroxymuconate Delta-isomerase
5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase (EC:4.1.1.68)
Alternative name(s):
5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
Short name:
OPET decarboxylase
Gene namesi
Name:hpaG
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4294294-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylasePRO_0000156833Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ46978.
SMRiQ46978. Positions 1-429.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 215215ApproximateAdd
BLAST
Repeati216 – 429214ApproximateAdd
BLAST

Sequence similaritiesi

Belongs to the FAH family.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di3.90.850.10. 2 hits.
InterProiIPR002529. Fumarylacetoacetase_C.
IPR011234. Fumarylacetoacetase_C-rel.
IPR012684. HPA_isomer/decarb_C.
IPR012686. HPA_isomer/decarb_N.
[Graphical view]
PfamiPF01557. FAA_hydrolase. 2 hits.
[Graphical view]
SUPFAMiSSF56529. SSF56529. 2 hits.
TIGRFAMsiTIGR02303. HpaG-C-term. 1 hit.
TIGR02305. HpaG-N-term. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46978-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGTIFAVAL NHRSQLDAWQ EAFQQSPYKA PPKTAVWFIK PRNTVIGCGE
60 70 80 90 100
PIPFPQGEKV LSGATVALIV GKTATKVREE DAAEYIAGYA LANDVSLPEE
110 120 130 140 150
SFYRPAIKAK CRDGFCPIGE TVALSNVDNL TIYTEINGRP ADHWNTADLQ
160 170 180 190 200
RNAAQLLSAL SEFATLNPGD AILLGTPQAR VEIQPGDRVR VLAEGFPPLE
210 220 230 240 250
NPVVDEREVT TRKSFPTLPH PHGTLFALGL NYADHASELE FKPPEEPLVF
260 270 280 290 300
LKAPNTLTGD NQTSVRPNNI EYMHYEAELV VVIGKQARNV SEADAMDYVA
310 320 330 340 350
GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPMLSTIVPK EAIPDPHNLT
360 370 380 390 400
LRTFVNGELR QQGTTADLIF SVPFLIAYLS EFMTLNPGDM IATGTPKGLS
410 420
DVVPGDEVVV EVEGVGRLVN RIVSEETAK
Length:429
Mass (Da):47,108
Last modified:November 1, 1996 - v1
Checksum:iBC8AC5E6FEB455B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37980 Genomic DNA. Translation: CAA86040.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37980 Genomic DNA. Translation: CAA86040.1 .

3D structure databases

ProteinModelPortali Q46978.
SMRi Q46978. Positions 1-429.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00208 ; UER00419 .
UPA00208 ; UER00420 .
BioCyci MetaCyc:MONOMER-604.

Family and domain databases

Gene3Di 3.90.850.10. 2 hits.
InterProi IPR002529. Fumarylacetoacetase_C.
IPR011234. Fumarylacetoacetase_C-rel.
IPR012684. HPA_isomer/decarb_C.
IPR012686. HPA_isomer/decarb_N.
[Graphical view ]
Pfami PF01557. FAA_hydrolase. 2 hits.
[Graphical view ]
SUPFAMi SSF56529. SSF56529. 2 hits.
TIGRFAMsi TIGR02303. HpaG-C-term. 1 hit.
TIGR02305. HpaG-N-term. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster."
    Prieto M.A., Diaz E., Garcia J.L.
    J. Bacteriol. 178:111-120(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W / ATCC 11105 / DSM 1900.

Entry informationi

Entry nameiHPAG_ECOLX
AccessioniPrimary (citable) accession number: Q46978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3