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Q46978

- HPAG_ECOLX

UniProt

Q46978 - HPAG_ECOLX

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Protein

4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase

Gene
hpaG
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate).

Catalytic activityi

5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate.
5-oxopent-3-ene-1,2,5-tricarboxylate = 2-oxohept-3-enedioate + CO2.

Cofactori

Magnesium By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi276 – 2761Divalent metal cation By similarity
Metal bindingi278 – 2781Divalent metal cation By similarity
Metal bindingi307 – 3071Divalent metal cation By similarity

GO - Molecular functioni

  1. 5-carboxymethyl-2-hydroxymuconate delta-isomerase activity Source: UniProtKB-EC
  2. 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 4-hydroxyphenylacetate catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-604.
UniPathwayiUPA00208; UER00419.
UPA00208; UER00420.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase
Including the following 2 domains:
2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC:5.3.3.10)
Short name:
HHDD isomerase
Alternative name(s):
5-carboxymethyl-2-hydroxymuconate Delta-isomerase
5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase (EC:4.1.1.68)
Alternative name(s):
5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
Short name:
OPET decarboxylase
Gene namesi
Name:hpaG
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4294294-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylasePRO_0000156833Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ46978.
SMRiQ46978. Positions 1-429.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 215215ApproximateAdd
BLAST
Repeati216 – 429214ApproximateAdd
BLAST

Sequence similaritiesi

Belongs to the FAH family.

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di3.90.850.10. 2 hits.
InterProiIPR002529. Fumarylacetoacetase_C.
IPR011234. Fumarylacetoacetase_C-rel.
IPR012684. HPA_isomer/decarb_C.
IPR012686. HPA_isomer/decarb_N.
[Graphical view]
PfamiPF01557. FAA_hydrolase. 2 hits.
[Graphical view]
SUPFAMiSSF56529. SSF56529. 2 hits.
TIGRFAMsiTIGR02303. HpaG-C-term. 1 hit.
TIGR02305. HpaG-N-term. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46978-1 [UniParc]FASTAAdd to Basket

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MKGTIFAVAL NHRSQLDAWQ EAFQQSPYKA PPKTAVWFIK PRNTVIGCGE    50
PIPFPQGEKV LSGATVALIV GKTATKVREE DAAEYIAGYA LANDVSLPEE 100
SFYRPAIKAK CRDGFCPIGE TVALSNVDNL TIYTEINGRP ADHWNTADLQ 150
RNAAQLLSAL SEFATLNPGD AILLGTPQAR VEIQPGDRVR VLAEGFPPLE 200
NPVVDEREVT TRKSFPTLPH PHGTLFALGL NYADHASELE FKPPEEPLVF 250
LKAPNTLTGD NQTSVRPNNI EYMHYEAELV VVIGKQARNV SEADAMDYVA 300
GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPMLSTIVPK EAIPDPHNLT 350
LRTFVNGELR QQGTTADLIF SVPFLIAYLS EFMTLNPGDM IATGTPKGLS 400
DVVPGDEVVV EVEGVGRLVN RIVSEETAK 429
Length:429
Mass (Da):47,108
Last modified:November 1, 1996 - v1
Checksum:iBC8AC5E6FEB455B1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z37980 Genomic DNA. Translation: CAA86040.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z37980 Genomic DNA. Translation: CAA86040.1 .

3D structure databases

ProteinModelPortali Q46978.
SMRi Q46978. Positions 1-429.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00208 ; UER00419 .
UPA00208 ; UER00420 .
BioCyci MetaCyc:MONOMER-604.

Family and domain databases

Gene3Di 3.90.850.10. 2 hits.
InterProi IPR002529. Fumarylacetoacetase_C.
IPR011234. Fumarylacetoacetase_C-rel.
IPR012684. HPA_isomer/decarb_C.
IPR012686. HPA_isomer/decarb_N.
[Graphical view ]
Pfami PF01557. FAA_hydrolase. 2 hits.
[Graphical view ]
SUPFAMi SSF56529. SSF56529. 2 hits.
TIGRFAMsi TIGR02303. HpaG-C-term. 1 hit.
TIGR02305. HpaG-N-term. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster."
    Prieto M.A., Diaz E., Garcia J.L.
    J. Bacteriol. 178:111-120(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W / ATCC 11105 / DSM 1900.

Entry informationi

Entry nameiHPAG_ECOLX
AccessioniPrimary (citable) accession number: Q46978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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