Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q46978

- HPAG_ECOLX

UniProt

Q46978 - HPAG_ECOLX

Protein

4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase

Gene

hpaG

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate).

    Catalytic activityi

    5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate.
    5-oxopent-3-ene-1,2,5-tricarboxylate = 2-oxohept-3-enedioate + CO2.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi276 – 2761Divalent metal cationBy similarity
    Metal bindingi278 – 2781Divalent metal cationBy similarity
    Metal bindingi307 – 3071Divalent metal cationBy similarity

    GO - Molecular functioni

    1. 5-carboxymethyl-2-hydroxymuconate delta-isomerase activity Source: UniProtKB-EC
    2. 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 4-hydroxyphenylacetate catabolic process Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Lyase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-604.
    UniPathwayiUPA00208; UER00419.
    UPA00208; UER00420.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase
    Including the following 2 domains:
    2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC:5.3.3.10)
    Short name:
    HHDD isomerase
    Alternative name(s):
    5-carboxymethyl-2-hydroxymuconate Delta-isomerase
    5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase (EC:4.1.1.68)
    Alternative name(s):
    5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
    Short name:
    OPET decarboxylase
    Gene namesi
    Name:hpaG
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4294294-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylasePRO_0000156833Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ46978.
    SMRiQ46978. Positions 1-429.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1 – 215215ApproximateAdd
    BLAST
    Repeati216 – 429214ApproximateAdd
    BLAST

    Sequence similaritiesi

    Belongs to the FAH family.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di3.90.850.10. 2 hits.
    InterProiIPR002529. Fumarylacetoacetase_C.
    IPR011234. Fumarylacetoacetase_C-rel.
    IPR012684. HPA_isomer/decarb_C.
    IPR012686. HPA_isomer/decarb_N.
    [Graphical view]
    PfamiPF01557. FAA_hydrolase. 2 hits.
    [Graphical view]
    SUPFAMiSSF56529. SSF56529. 2 hits.
    TIGRFAMsiTIGR02303. HpaG-C-term. 1 hit.
    TIGR02305. HpaG-N-term. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q46978-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGTIFAVAL NHRSQLDAWQ EAFQQSPYKA PPKTAVWFIK PRNTVIGCGE    50
    PIPFPQGEKV LSGATVALIV GKTATKVREE DAAEYIAGYA LANDVSLPEE 100
    SFYRPAIKAK CRDGFCPIGE TVALSNVDNL TIYTEINGRP ADHWNTADLQ 150
    RNAAQLLSAL SEFATLNPGD AILLGTPQAR VEIQPGDRVR VLAEGFPPLE 200
    NPVVDEREVT TRKSFPTLPH PHGTLFALGL NYADHASELE FKPPEEPLVF 250
    LKAPNTLTGD NQTSVRPNNI EYMHYEAELV VVIGKQARNV SEADAMDYVA 300
    GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPMLSTIVPK EAIPDPHNLT 350
    LRTFVNGELR QQGTTADLIF SVPFLIAYLS EFMTLNPGDM IATGTPKGLS 400
    DVVPGDEVVV EVEGVGRLVN RIVSEETAK 429
    Length:429
    Mass (Da):47,108
    Last modified:November 1, 1996 - v1
    Checksum:iBC8AC5E6FEB455B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z37980 Genomic DNA. Translation: CAA86040.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z37980 Genomic DNA. Translation: CAA86040.1 .

    3D structure databases

    ProteinModelPortali Q46978.
    SMRi Q46978. Positions 1-429.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00208 ; UER00419 .
    UPA00208 ; UER00420 .
    BioCyci MetaCyc:MONOMER-604.

    Family and domain databases

    Gene3Di 3.90.850.10. 2 hits.
    InterProi IPR002529. Fumarylacetoacetase_C.
    IPR011234. Fumarylacetoacetase_C-rel.
    IPR012684. HPA_isomer/decarb_C.
    IPR012686. HPA_isomer/decarb_N.
    [Graphical view ]
    Pfami PF01557. FAA_hydrolase. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56529. SSF56529. 2 hits.
    TIGRFAMsi TIGR02303. HpaG-C-term. 1 hit.
    TIGR02305. HpaG-N-term. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of Escherichia coli W: engineering a mobile aromatic degradative cluster."
      Prieto M.A., Diaz E., Garcia J.L.
      J. Bacteriol. 178:111-120(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: W / ATCC 11105 / DSM 1900.

    Entry informationi

    Entry nameiHPAG_ECOLX
    AccessioniPrimary (citable) accession number: Q46978
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3