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Q46944

- Q46944_ECOLX

UniProt

Q46944 - Q46944_ECOLX

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Protein
Submitted name: Eco29kIR
Gene
eco29kIR
Organism
Escherichia coli
Status
Unreviewed - Annotation score: 1 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561Phosphate 1Imported
Binding sitei68 – 681Phosphate 1Imported
Binding sitei194 – 1941Phosphate 2Imported
Binding sitei203 – 2031Phosphate 3Imported

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    EndonucleaseImported, Hydrolase, Nuclease

    Protein family/group databases

    REBASEi2171. Eco29kI.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Eco29kIRImported
    Submitted name:
    Restriction endonucleaseImported (EC:3.1.21.4Imported)
    Gene namesi
    Name:eco29kIRImported
    Encoded oniPlasmid pECO29Imported
    OrganismiEscherichia coliImported
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-59011N.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MX1X-ray2.30A2-214[»]
    3MX4X-ray2.50A/B/C/D/E/F/G/H2-214[»]
    3NICX-ray2.80A/B/C/D/E/F/G/H2-214[»]

    Family & Domainsi

    Family and domain databases

    InterProiIPR018575. Restrct_endonuc_II_Eco29kI.
    [Graphical view]
    PfamiPF09517. RE_Eco29kI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46944-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHNKKFDRSE HVYRNDSFLE LIKDAVRFFS GTPVHSLPPP ERFQGAGVYA    50
    LYYTGHYSLY DEYSRINRLA YNLPIYVGKA VPAGWRQSRI SDHETRAGSE 100
    LSNRIREHGR NIAKTSNLDL CDFSCRFVIF EATGSDMIST VEAALIKIYK 150
    PLWNTVVDGF GNHTPGAGRF AQAKSDWDVI HPGREWAEKC TGVHSEPYFI 200
    EERIKQYFSK SNFT 214
    Length:214
    Mass (Da):24,567
    Last modified:November 1, 1996 - v1
    Checksum:i4899A02F9A9C3ECA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L76268 Genomic DNA. Translation: AAC37144.1.
    AJ001708 Genomic DNA. Translation: CAA04943.1.
    RefSeqiNP_052187.1. NC_001537.1.
    WP_001529705.1. NC_001537.1.

    Genome annotation databases

    GeneIDi1238552.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L76268 Genomic DNA. Translation: AAC37144.1 .
    AJ001708 Genomic DNA. Translation: CAA04943.1 .
    RefSeqi NP_052187.1. NC_001537.1.
    WP_001529705.1. NC_001537.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3MX1 X-ray 2.30 A 2-214 [» ]
    3MX4 X-ray 2.50 A/B/C/D/E/F/G/H 2-214 [» ]
    3NIC X-ray 2.80 A/B/C/D/E/F/G/H 2-214 [» ]
    ModBasei Search...

    Protein-protein interaction databases

    DIPi DIP-59011N.

    Protein family/group databases

    REBASEi 2171. Eco29kI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1238552.

    Family and domain databases

    InterProi IPR018575. Restrct_endonuc_II_Eco29kI.
    [Graphical view ]
    Pfami PF09517. RE_Eco29kI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Eco29kI, a novel plasmid encoded restriction endonuclease from Escherichia coli."
      Pertzev A.V., Ruban N.M., Zakharova M.V., Beletzkaja I.V., Petrov S.I., Kravetz A.N., Solonin A.S.
      Nucleic Acids Res. 20:1991-1991(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 29kImported.
      Plasmid: pECO29
    2. Solonin A.S.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 29kImported.
      Plasmid: pECO29
    3. "Cloning and sequence analysis of the plasmid-borne genes encoding the Eco29kI restriction and modification enzymes."
      Zakharova M.V., Beletskaya I.V., Kravetz A.N., Pertzev A.V., Mayorov S.G., Shlyapnikov M.G., Solonin A.S.
      Gene 208:177-182(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    4. "Folding, DNA recognition, and function of GIY-YIG endonucleases: crystal structures of R.Eco29kI."
      Mak A.N., Lambert A.R., Stoddard B.L.
      Structure 18:1321-1331(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 2-214 IN COMPLEX WITH PHOSPHATE.

    Entry informationi

    Entry nameiQ46944_ECOLX
    AccessioniPrimary (citable) accession number: Q46944
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1996
    Last sequence update: November 1, 1996
    Last modified: September 3, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, PlasmidImported

    External Data

    Dasty 3

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