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Q46944

- Q46944_ECOLX

UniProt

Q46944 - Q46944_ECOLX

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Protein
Submitted name:

Eco29kIR

Gene
eco29kIR
Organism
Escherichia coli
Status
Unreviewed - Annotation score: 1 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561Phosphate 1Imported
Binding sitei68 – 681Phosphate 1Imported
Binding sitei194 – 1941Phosphate 2Imported
Binding sitei203 – 2031Phosphate 3Imported

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseImported, Hydrolase, Nuclease

Protein family/group databases

REBASEi2171. Eco29kI.

Names & Taxonomyi

Protein namesi
Submitted name:
Eco29kIRImported
Submitted name:
Restriction endonucleaseImported (EC:3.1.21.4Imported)
Gene namesi
Name:eco29kIRImported
Encoded oniPlasmid pECO29Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Interactioni

Protein-protein interaction databases

DIPiDIP-59011N.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MX1X-ray2.30A2-214[»]
3MX4X-ray2.50A/B/C/D/E/F/G/H2-214[»]
3NICX-ray2.80A/B/C/D/E/F/G/H2-214[»]

Family & Domainsi

Family and domain databases

InterProiIPR018575. Restrct_endonuc_II_Eco29kI.
[Graphical view]
PfamiPF09517. RE_Eco29kI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46944-1 [UniParc]FASTAAdd to Basket

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MHNKKFDRSE HVYRNDSFLE LIKDAVRFFS GTPVHSLPPP ERFQGAGVYA    50
LYYTGHYSLY DEYSRINRLA YNLPIYVGKA VPAGWRQSRI SDHETRAGSE 100
LSNRIREHGR NIAKTSNLDL CDFSCRFVIF EATGSDMIST VEAALIKIYK 150
PLWNTVVDGF GNHTPGAGRF AQAKSDWDVI HPGREWAEKC TGVHSEPYFI 200
EERIKQYFSK SNFT 214
Length:214
Mass (Da):24,567
Last modified:November 1, 1996 - v1
Checksum:i4899A02F9A9C3ECA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L76268 Genomic DNA. Translation: AAC37144.1.
AJ001708 Genomic DNA. Translation: CAA04943.1.
RefSeqiNP_052187.1. NC_001537.1.
WP_001529705.1. NC_001537.1.

Genome annotation databases

GeneIDi1238552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L76268 Genomic DNA. Translation: AAC37144.1 .
AJ001708 Genomic DNA. Translation: CAA04943.1 .
RefSeqi NP_052187.1. NC_001537.1.
WP_001529705.1. NC_001537.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MX1 X-ray 2.30 A 2-214 [» ]
3MX4 X-ray 2.50 A/B/C/D/E/F/G/H 2-214 [» ]
3NIC X-ray 2.80 A/B/C/D/E/F/G/H 2-214 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59011N.

Protein family/group databases

REBASEi 2171. Eco29kI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1238552.

Family and domain databases

InterProi IPR018575. Restrct_endonuc_II_Eco29kI.
[Graphical view ]
Pfami PF09517. RE_Eco29kI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Eco29kI, a novel plasmid encoded restriction endonuclease from Escherichia coli."
    Pertzev A.V., Ruban N.M., Zakharova M.V., Beletzkaja I.V., Petrov S.I., Kravetz A.N., Solonin A.S.
    Nucleic Acids Res. 20:1991-1991(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 29kImported.
    Plasmid: pECO29
  2. Solonin A.S.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 29kImported.
    Plasmid: pECO29
  3. "Cloning and sequence analysis of the plasmid-borne genes encoding the Eco29kI restriction and modification enzymes."
    Zakharova M.V., Beletskaya I.V., Kravetz A.N., Pertzev A.V., Mayorov S.G., Shlyapnikov M.G., Solonin A.S.
    Gene 208:177-182(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  4. "Folding, DNA recognition, and function of GIY-YIG endonucleases: crystal structures of R.Eco29kI."
    Mak A.N., Lambert A.R., Stoddard B.L.
    Structure 18:1321-1331(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 2-214 IN COMPLEX WITH PHOSPHATE.

Entry informationi

Entry nameiQ46944_ECOLX
AccessioniPrimary (citable) accession number: Q46944
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, PlasmidImported

External Data

Dasty 3

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