Q46939 (YQEF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable acetyl-CoA acetyltransferase
EC=2.3.1.9

Alternative name(s):
Acetoacetyl-CoA thiolase

Gene names

Name:	yqeF
Ordered Locus Names:	b2844, JW5453

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the thiolase family.
Sequence caution	The sequence AAB40491.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC acetyl-CoA C-acyltransferase activity Inferred from mutant phenotype PubMed 21832992. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Probable acetyl-CoA acetyltransferase		PRO_0000206456
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
	Active site	348	1	Proton acceptor By similarity
	Active site	378	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q46939 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: FF21A11064A9B88B	FASTA	393	41,019
	10 20 30 40 50 60 MKDVVIVGAL RTPIGCFRGA LAGHSAVELG SLVVKALIER TGVPAYAVDE VILGQVLTAG 70 80 90 100 110 120 AGQNPARQSA IKGGLPNSVS AITINDVCGS GLKALHLATQ AIQCGEADIV IAGGQENMSR 130 140 150 160 170 180 APHVLTDSRT GAQLGNSQLV DSLVHDGLWD AFNDYHIGVT AENLAREYGI SRQLQDAYAL 190 200 210 220 230 240 SSQQKARAAI DAGRFKDEIV PVMTQSNGQT LVVDTDEQPR TDASAEGLAR LNPSFDSLGS 250 260 270 280 290 300 VTAGNASSIN DGAAAVMMMS EAKARALNLP VLARIRAFAS VGVDPALMGI APVYATRRCL 310 320 330 340 350 360 ERVGWQLAEV DLIEANEAFA AQALSVGKML EWDERRVNVN GGAIALGHPI GASGCRILVS 370 380 390 LVHEMVKRNA RKGLATLCIG GGQGVALTIE RDE

References

[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29581 Genomic DNA. Translation: AAB40491.1. Different initiation. U00096 Genomic DNA. Translation: AAC75883.2. AP009048 Genomic DNA. Translation: BAE76913.1.
PIR	E65067.
RefSeq	NP_417321.2. NC_000913.2. YP_491049.1. NC_007779.1.
3D structure databases
ProteinModelPortal	Q46939.
SMR	Q46939. Positions 4-390.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12853N.
STRING	511145.b2844.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75883; AAC75883; b2844. BAE76913; BAE76913; BAE76913.
GeneID	12934518. 947324.
KEGG	ecj:Y75_p2778. eco:b2844.
PATRIC	32121106. VBIEscCol129921_2942.
Organism-specific databases
EchoBASE	EB2900.
EcoGene	EG13097. yqeF.
Phylogenomic databases
eggNOG	COG0183.
HOGENOM	HOG000012238.
KO	K00626.
OMA	MMKDVVI.
ProtClustDB	PRK05790.
Enzyme and pathway databases
BioCyc	EcoCyc:G7464-MONOMER. ECOL316407:JW5453-MONOMER.
Gene expression databases
Genevestigator	Q46939.
Family and domain databases
Gene3D	3.40.47.10. 4 hits.
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
PANTHER	PTHR18919. PTHR18919. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YQEF_ECOLI

Accession

Primary (citable) accession number: Q46939
Secondary accession number(s): Q2M9Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program