Q46920 (QUEF_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 103.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: NADPH-dependent 7-cyano-7-deazaguanine reductase EC=1.7.1.13 Alternative name(s): 7-cyano-7-carbaguanine reductase NADPH-dependent nitrile oxidoreductase PreQ(0) reductase | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Reference proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › ![]() |
Protein attributes
| Sequence length | 282 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). Ref.3 |
| Catalytic activity | 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817 |
| Pathway | tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817 |
| Subunit structure | Homodimer. Ref.3 |
| Subcellular location | Cytoplasm Probable HAMAP-Rule MF_00817. |
| Sequence similarities | Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily. |
| Biophysicochemical properties | Kinetic parameters: KM=36 µM for NADPH Ref.3 |
Ontologies
| Keywords | |
|---|---|
| Biological process | Queuosine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | queuosine biosynthetic process Inferred from mutant phenotype Ref.3. Source: EcoCyc |
| Cellular_component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activityInferred from direct assay Ref.3. Source: EcoCyc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 282 | 282 | NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817 | PRO_0000163031 | |||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U29581 Genomic DNA. Translation: AAB40444.1. U00096 Genomic DNA. Translation: AAC75836.1. AP009048 Genomic DNA. Translation: BAE76866.1. |
| PIR | F65061. |
| RefSeq | NP_417274.1. NC_000913.2. YP_491002.1. NC_007779.1. |
3D structure databases | |
| ProteinModelPortal | Q46920. |
| SMR | Q46920. Positions 23-282. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-12848N. |
| IntAct | Q46920. 6 interactions. |
| MINT | MINT-1288325. |
| STRING | 511145.b2794. |
Proteomic databases | |
| PaxDb | Q46920. |
| PRIDE | Q46920. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAC75836; AAC75836; b2794. BAE76866; BAE76866; BAE76866. |
| GeneID | 12933155. 947270. |
| KEGG | ecj:Y75_p2731. eco:b2794. |
| PATRIC | 32121004. VBIEscCol129921_2894. |
Organism-specific databases | |
| EchoBASE | EB2965. |
| EcoGene | EG13173. queF. |
Phylogenomic databases | |
| eggNOG | COG0780. |
| HOGENOM | HOG000273755. |
| KO | K06879. |
| OMA | ERIFCDL. |
| ProtClustDB | PRK11792. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:G7452-MONOMER. ECOL316407:JW2765-MONOMER. MetaCyc:G7452-MONOMER. |
| UniPathway | UPA00392. |
Gene expression databases | |
| Genevestigator | Q46920. |
Family and domain databases | |
| HAMAP | MF_00817. QueF_type2. |
| InterPro | IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view] |
| Pfam | PF01227. GTP_cyclohydroI. 1 hit. [Graphical view] |
| PIRSF | PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit. |
| TIGRFAMs | TIGR03138. QueF. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | QUEF_ECOLI | ||||||||
| Accession | Primary (citable) accession number: Q46920 Secondary accession number(s): Q2MA40 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
