Reviewed, UniProtKB/Swiss-Prot Q46920 (QUEF_ECOLI)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Synonyms:	yqcD
Ordered Locus Names:	b2794, JW2765

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). Ref.3
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00817
Subunit structure	Homodimer. Ref.3
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.
biophysicochemical properties	Kinetic parameters: KM=36 µM for NADPH HAMAP MF_00817

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000163031

Sequences

	Sequence		Length	Mass (Da)
	Q46920-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3C22B9BA7AA3C79D	FASTA	282	32,588
	10 20 30 40 50 60 MSSYANHQAL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP FHGTDIWTLY 70 80 90 100 110 120 ELSWLNAKGL PQVAVGHVEL DYTSVNLIES KSFKLYLNSF NQTRFNNWDE VRQTLERDLS 130 140 150 160 170 180 TCAQGKISVA LYRLDELEGQ PIGHFNGTCI DDQDITIDNY EFTTDYLENA TCGEKVVEET 190 200 210 220 230 240 LVSHLLKSNC LITHQPDWGS LQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL 250 260 270 280 LRFCQPEKLS VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold." Van Lanen S.G., Reader J.S., Swairjo M.A., de Crecy-Lagard V., Lee B., Iwata-Reuyl D. Proc. Natl. Acad. Sci. U.S.A. 102:4264-4269(2005) [PubMed: 15767583] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U29581 Genomic DNA. Translation: AAB40444.1. U00096 Genomic DNA. Translation: AAC75836.1. AP009048 Genomic DNA. Translation: BAE76866.1.
PIR	F65061.
RefSeq	AP_003360.1. NP_417274.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:12848N.
Genome annotation databases
GeneID	947270.
GenomeReviews	Gene locus JW2765 in contig AP009048_GR. Gene locus b2794 in contig U00096_GR.
KEGG	ecj:JW2765. eco:b2794.
Organism-specific databases
EchoBASE	EB2965.
EcoGene	EG13173. queF.
CMR	Search...
Phylogenomic databases
HOGENOM	Q46920.
OMA	Q46920. EHNEFHE.
Enzyme and pathway databases
BioCyc	EcoCyc:G7452-MON. MetaCyc:G7452-MON.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR001474. GTP_CycHdrlase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_ECOLI

Accession

Primary (citable) accession number: Q46920
Secondary accession number(s): Q2MA40

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)