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Reviewed, UniProtKB/Swiss-Prot Q46920 (QUEF_ECOLI)

Last modified June 16, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase
Gene names
Name: queF
Synonyms: yqcD
Ordered Locus Names: b2794, JW2765
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). Ref.3

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817

Pathway

tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00817

Subunit structure

Homodimer. Ref.3

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

biophysicochemical properties

Kinetic parameters:

KM=36 µM for NADPH HAMAP MF_00817

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817
PRO_0000163031

Sequences

Sequence LengthMass (Da)Tools
Q46920-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3C22B9BA7AA3C79D

FASTA28232,588
        10         20         30         40         50         60 
MSSYANHQAL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP FHGTDIWTLY 

        70         80         90        100        110        120 
ELSWLNAKGL PQVAVGHVEL DYTSVNLIES KSFKLYLNSF NQTRFNNWDE VRQTLERDLS 

       130        140        150        160        170        180 
TCAQGKISVA LYRLDELEGQ PIGHFNGTCI DDQDITIDNY EFTTDYLENA TCGEKVVEET 

       190        200        210        220        230        240 
LVSHLLKSNC LITHQPDWGS LQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL 

       250        260        270        280 
LRFCQPEKLS VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold."
Van Lanen S.G., Reader J.S., Swairjo M.A., de Crecy-Lagard V., Lee B., Iwata-Reuyl D.
Proc. Natl. Acad. Sci. U.S.A. 102:4264-4269(2005) [PubMed: 15767583] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases

U29581 Genomic DNA. Translation: AAB40444.1.
U00096 Genomic DNA. Translation: AAC75836.1.
AP009048 Genomic DNA. Translation: BAE76866.1.
PIRF65061.
RefSeqAP_003360.1.
NP_417274.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:12848N.

Genome annotation databases

GeneID947270.
GenomeReviewsGene locus JW2765 in contig AP009048_GR.
Gene locus b2794 in contig U00096_GR.
KEGGecj:JW2765.
eco:b2794.

Organism-specific databases

EchoBASEEB2965.
EcoGeneEG13173. queF.
CMRSearch...

Phylogenomic databases

HOGENOMQ46920.
OMAQ46920. EHNEFHE.

Enzyme and pathway databases

BioCycEcoCyc:G7452-MON.
MetaCyc:G7452-MON.

Family and domain databases

HAMAPMF_00817.
[Tree]
InterProIPR016428. CN_OxRdtase_NADPH-dep_YqcD.
IPR001474. GTP_CycHdrlase_I.
[Graphical view]
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsTIGR03138. QueF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_ECOLI
AccessionPrimary (citable) accession number: Q46920
Secondary accession number(s): Q2MA40
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents