Q46920 (QUEF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Synonyms:	yqcD
Ordered Locus Names:	b2794, JW2765

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1). Ref.3
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817
Subunit structure	Homodimer. Ref.3
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.
Biophysicochemical properties	Kinetic parameters: KM=36 µM for NADPH Ref.3

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from mutant phenotype Ref.3. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activity Inferred from direct assay Ref.3. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817		PRO_0000163031

Sequences

	Sequence		Length	Mass (Da)
	Q46920 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3C22B9BA7AA3C79D	FASTA	282	32,588
	10 20 30 40 50 60 MSSYANHQAL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP FHGTDIWTLY 70 80 90 100 110 120 ELSWLNAKGL PQVAVGHVEL DYTSVNLIES KSFKLYLNSF NQTRFNNWDE VRQTLERDLS 130 140 150 160 170 180 TCAQGKISVA LYRLDELEGQ PIGHFNGTCI DDQDITIDNY EFTTDYLENA TCGEKVVEET 190 200 210 220 230 240 LVSHLLKSNC LITHQPDWGS LQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL 250 260 270 280 LRFCQPEKLS VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold." Van Lanen S.G., Reader J.S., Swairjo M.A., de Crecy-Lagard V., Lee B., Iwata-Reuyl D. Proc. Natl. Acad. Sci. U.S.A. 102:4264-4269(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29581 Genomic DNA. Translation: AAB40444.1. U00096 Genomic DNA. Translation: AAC75836.1. AP009048 Genomic DNA. Translation: BAE76866.1.
PIR	F65061.
RefSeq	NP_417274.1. NC_000913.2. YP_491002.1. NC_007779.1.
3D structure databases
ProteinModelPortal	Q46920.
SMR	Q46920. Positions 23-282.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12848N.
IntAct	Q46920. 6 interactions.
MINT	MINT-1288325.
STRING	511145.b2794.
Proteomic databases
PaxDb	Q46920.
PRIDE	Q46920.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75836; AAC75836; b2794. BAE76866; BAE76866; BAE76866.
GeneID	12933155. 947270.
KEGG	ecj:Y75_p2731. eco:b2794.
PATRIC	32121004. VBIEscCol129921_2894.
Organism-specific databases
EchoBASE	EB2965.
EcoGene	EG13173. queF.
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HOG000273755.
KO	K06879.
OMA	ERIFCDL.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	EcoCyc:G7452-MONOMER. ECOL316407:JW2765-MONOMER. MetaCyc:G7452-MONOMER.
UniPathway	UPA00392.
Gene expression databases
Genevestigator	Q46920.
Family and domain databases
HAMAP	MF_00817. QueF_type2.
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_ECOLI

Accession

Primary (citable) accession number: Q46920
Secondary accession number(s): Q2MA40

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program