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Q46915

- GUDX_ECOLI

UniProt

Q46915 - GUDX_ECOLI

Protein

Glucarate dehydratase-related protein

Gene

gudX

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Does not seem to have an in-vivo activity on glucarate or idarate. Its real substrate is unknown.

    Cofactori

    Divalent metal cations.By similarity

    Kineticsi

    1. KM=170 µM for idarate1 Publication
    2. KM=320 µM for glucarate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311SubstrateBy similarity
    Binding sitei104 – 1041SubstrateBy similarity
    Binding sitei149 – 1491SubstrateBy similarity
    Binding sitei204 – 2041SubstrateBy similarity
    Active sitei206 – 2061Proton acceptorSequence Analysis
    Metal bindingi234 – 2341MagnesiumSequence Analysis
    Metal bindingi265 – 2651MagnesiumSequence Analysis
    Metal bindingi288 – 2881MagnesiumSequence Analysis
    Binding sitei288 – 2881SubstrateBy similarity
    Active sitei338 – 3381Proton acceptorSequence Analysis
    Binding sitei367 – 3671SubstrateBy similarity
    Binding sitei421 – 4211SubstrateBy similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular amino acid catabolic process Source: InterPro
    2. cellular catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G7446-MONOMER.
    ECOL316407:JW2759-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucarate dehydratase-related protein (EC:4.2.1.-)
    Short name:
    GDH-RP
    Short name:
    GlucDRP
    Gene namesi
    Name:gudX
    Synonyms:ygcY
    Ordered Locus Names:b2788, JW2759
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13168. gudX.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446Glucarate dehydratase-related proteinPRO_0000171266Add
    BLAST

    Proteomic databases

    PRIDEiQ46915.

    Expressioni

    Gene expression databases

    GenevestigatoriQ46915.

    Interactioni

    Protein-protein interaction databases

    IntActiQ46915. 3 interactions.
    STRINGi511145.b2788.

    Structurei

    Secondary structure

    1
    446
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 2013
    Beta strandi24 – 263
    Beta strandi33 – 4412
    Beta strandi49 – 557
    Helixi58 – 669
    Helixi68 – 714
    Helixi76 – 783
    Helixi79 – 9012
    Helixi91 – 988
    Turni99 – 1013
    Helixi104 – 1063
    Helixi107 – 12721
    Helixi131 – 1344
    Beta strandi142 – 1465
    Beta strandi148 – 1503
    Helixi156 – 1583
    Beta strandi169 – 1713
    Helixi173 – 1764
    Helixi184 – 19815
    Beta strandi201 – 2066
    Beta strandi208 – 2103
    Helixi212 – 22514
    Beta strandi229 – 2346
    Helixi241 – 2477
    Turni248 – 2503
    Turni252 – 2543
    Beta strandi256 – 2605
    Helixi270 – 28112
    Beta strandi285 – 2906
    Helixi294 – 3029
    Beta strandi307 – 3104
    Helixi313 – 3164
    Helixi318 – 33013
    Helixi344 – 35512
    Helixi368 – 3714
    Turni372 – 3743
    Beta strandi378 – 3803
    Beta strandi388 – 3903
    Beta strandi394 – 3963
    Helixi403 – 41412
    Helixi424 – 4307

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GYPX-ray2.10C/D2-446[»]
    4IL0X-ray2.80A/B/C/D/E/F/G/H1-446[»]
    ProteinModelPortaliQ46915.
    SMRiQ46915. Positions 6-445.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni234 – 2363Substrate bindingBy similarity
    Regioni338 – 3403Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG4948.
    HOGENOMiHOG000238021.
    KOiK13918.
    OMAiREMNHAL.
    OrthoDBiEOG6V7BH9.
    PhylomeDBiQ46915.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    PROSITEiPS00908. MR_MLE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46915-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATQSSPVIT DMKVIPVAGH DSMLLNIGGA HNAYFTRNIV VLTDNAGHTG    50
    IGEAPGGDVI YQTLVDAIPM VLGQEVARLN KVVQQVHKGN QAADFDTFGK 100
    GAWTFELRVN AVAALEAALL DLLGKALNVP VCELLGPGKQ REAITVLGYL 150
    FYIGDRTKTD LPYVENTPGN HEWYQLRHQK AMNSEAVVRL AEASQDRYGF 200
    KDFKLKGGVL PGEQEIDTVR ALKKRFPDAR ITVDPNGAWL LDEAISLCKG 250
    LNDVLTYAED PCGAEQGFSG REVMAEFRRA TGLPVATNMI ATNWREMGHA 300
    VMLNAVDIPL ADPHFWTLSG AVRVAQLCDD WGLTWGCHSN NHFDISLAMF 350
    THVGAAAPGN PTAIDTHWIW QEGDCRLTQN PLEIKNGKIA VPDAPGLGVE 400
    LDWEQVQKAH EAYKRLPGGA RNDAGPMQYL IPGWTFDRKR PVFGRH 446
    Length:446
    Mass (Da):48,850
    Last modified:November 1, 1996 - v1
    Checksum:i9010C7FAAE4FBE49
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U29581 Genomic DNA. Translation: AAB40438.1.
    U00096 Genomic DNA. Translation: AAC75830.1.
    AP009048 Genomic DNA. Translation: BAE76860.1.
    PIRiH65060.
    RefSeqiNP_417268.1. NC_000913.3.
    YP_490996.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75830; AAC75830; b2788.
    BAE76860; BAE76860; BAE76860.
    GeneIDi12931905.
    947261.
    KEGGiecj:Y75_p2725.
    eco:b2788.
    PATRICi32120992. VBIEscCol129921_2888.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U29581 Genomic DNA. Translation: AAB40438.1 .
    U00096 Genomic DNA. Translation: AAC75830.1 .
    AP009048 Genomic DNA. Translation: BAE76860.1 .
    PIRi H65060.
    RefSeqi NP_417268.1. NC_000913.3.
    YP_490996.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GYP X-ray 2.10 C/D 2-446 [» ]
    4IL0 X-ray 2.80 A/B/C/D/E/F/G/H 1-446 [» ]
    ProteinModelPortali Q46915.
    SMRi Q46915. Positions 6-445.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q46915. 3 interactions.
    STRINGi 511145.b2788.

    Proteomic databases

    PRIDEi Q46915.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75830 ; AAC75830 ; b2788 .
    BAE76860 ; BAE76860 ; BAE76860 .
    GeneIDi 12931905.
    947261.
    KEGGi ecj:Y75_p2725.
    eco:b2788.
    PATRICi 32120992. VBIEscCol129921_2888.

    Organism-specific databases

    EchoBASEi EB2960.
    EcoGenei EG13168. gudX.

    Phylogenomic databases

    eggNOGi COG4948.
    HOGENOMi HOG000238021.
    KOi K13918.
    OMAi REMNHAL.
    OrthoDBi EOG6V7BH9.
    PhylomeDBi Q46915.

    Enzyme and pathway databases

    BioCyci EcoCyc:G7446-MONOMER.
    ECOL316407:JW2759-MONOMER.

    Miscellaneous databases

    PROi Q46915.

    Gene expression databases

    Genevestigatori Q46915.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProi IPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    [Graphical view ]
    PANTHERi PTHR13794. PTHR13794. 1 hit.
    Pfami PF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view ]
    SMARTi SM00922. MR_MLE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    PROSITEi PS00908. MR_MLE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli."
      Hubbard B.K., Koch M., Palmer D.R., Babbitt P.C., Gerlt J.A.
      Biochemistry 37:14369-14375(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiGUDX_ECOLI
    AccessioniPrimary (citable) accession number: Q46915
    Secondary accession number(s): Q2MA46
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3