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Q46915

- GUDX_ECOLI

UniProt

Q46915 - GUDX_ECOLI

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Protein
Glucarate dehydratase-related protein
Gene
gudX, ygcY, b2788, JW2759
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Does not seem to have an in-vivo activity on glucarate or idarate. Its real substrate is unknown.

Cofactori

Divalent metal cations By similarity.

Kineticsi

  1. KM=170 µM for idarate1 Publication
  2. KM=320 µM for glucarate

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311Substrate By similarity
Binding sitei104 – 1041Substrate By similarity
Binding sitei149 – 1491Substrate By similarity
Binding sitei204 – 2041Substrate By similarity
Active sitei206 – 2061Proton acceptor Reviewed prediction
Metal bindingi234 – 2341Magnesium Reviewed prediction
Metal bindingi265 – 2651Magnesium Reviewed prediction
Metal bindingi288 – 2881Magnesium Reviewed prediction
Binding sitei288 – 2881Substrate By similarity
Active sitei338 – 3381Proton acceptor Reviewed prediction
Binding sitei367 – 3671Substrate By similarity
Binding sitei421 – 4211Substrate By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular amino acid catabolic process Source: InterPro
  2. cellular catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7446-MONOMER.
ECOL316407:JW2759-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucarate dehydratase-related protein (EC:4.2.1.-)
Short name:
GDH-RP
Short name:
GlucDRP
Gene namesi
Name:gudX
Synonyms:ygcY
Ordered Locus Names:b2788, JW2759
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13168. gudX.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Glucarate dehydratase-related protein
PRO_0000171266Add
BLAST

Proteomic databases

PRIDEiQ46915.

Expressioni

Gene expression databases

GenevestigatoriQ46915.

Interactioni

Protein-protein interaction databases

IntActiQ46915. 3 interactions.
STRINGi511145.b2788.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 2013
Beta strandi24 – 263
Beta strandi33 – 4412
Beta strandi49 – 557
Helixi58 – 669
Helixi68 – 714
Helixi76 – 783
Helixi79 – 9012
Helixi91 – 988
Turni99 – 1013
Helixi104 – 1063
Helixi107 – 12721
Helixi131 – 1344
Beta strandi142 – 1465
Beta strandi148 – 1503
Helixi156 – 1583
Beta strandi169 – 1713
Helixi173 – 1764
Helixi184 – 19815
Beta strandi201 – 2066
Beta strandi208 – 2103
Helixi212 – 22514
Beta strandi229 – 2346
Helixi241 – 2477
Turni248 – 2503
Turni252 – 2543
Beta strandi256 – 2605
Helixi270 – 28112
Beta strandi285 – 2906
Helixi294 – 3029
Beta strandi307 – 3104
Helixi313 – 3164
Helixi318 – 33013
Helixi344 – 35512
Helixi368 – 3714
Turni372 – 3743
Beta strandi378 – 3803
Beta strandi388 – 3903
Beta strandi394 – 3963
Helixi403 – 41412
Helixi424 – 4307

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GYPX-ray2.10C/D2-446[»]
4IL0X-ray2.80A/B/C/D/E/F/G/H1-446[»]
ProteinModelPortaliQ46915.
SMRiQ46915. Positions 6-445.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni234 – 2363Substrate binding By similarity
Regioni338 – 3403Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4948.
HOGENOMiHOG000238021.
KOiK13918.
OMAiREMNHAL.
OrthoDBiEOG6V7BH9.
PhylomeDBiQ46915.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46915-1 [UniParc]FASTAAdd to Basket

« Hide

MATQSSPVIT DMKVIPVAGH DSMLLNIGGA HNAYFTRNIV VLTDNAGHTG    50
IGEAPGGDVI YQTLVDAIPM VLGQEVARLN KVVQQVHKGN QAADFDTFGK 100
GAWTFELRVN AVAALEAALL DLLGKALNVP VCELLGPGKQ REAITVLGYL 150
FYIGDRTKTD LPYVENTPGN HEWYQLRHQK AMNSEAVVRL AEASQDRYGF 200
KDFKLKGGVL PGEQEIDTVR ALKKRFPDAR ITVDPNGAWL LDEAISLCKG 250
LNDVLTYAED PCGAEQGFSG REVMAEFRRA TGLPVATNMI ATNWREMGHA 300
VMLNAVDIPL ADPHFWTLSG AVRVAQLCDD WGLTWGCHSN NHFDISLAMF 350
THVGAAAPGN PTAIDTHWIW QEGDCRLTQN PLEIKNGKIA VPDAPGLGVE 400
LDWEQVQKAH EAYKRLPGGA RNDAGPMQYL IPGWTFDRKR PVFGRH 446
Length:446
Mass (Da):48,850
Last modified:November 1, 1996 - v1
Checksum:i9010C7FAAE4FBE49
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U29581 Genomic DNA. Translation: AAB40438.1.
U00096 Genomic DNA. Translation: AAC75830.1.
AP009048 Genomic DNA. Translation: BAE76860.1.
PIRiH65060.
RefSeqiNP_417268.1. NC_000913.3.
YP_490996.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75830; AAC75830; b2788.
BAE76860; BAE76860; BAE76860.
GeneIDi12931905.
947261.
KEGGiecj:Y75_p2725.
eco:b2788.
PATRICi32120992. VBIEscCol129921_2888.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U29581 Genomic DNA. Translation: AAB40438.1 .
U00096 Genomic DNA. Translation: AAC75830.1 .
AP009048 Genomic DNA. Translation: BAE76860.1 .
PIRi H65060.
RefSeqi NP_417268.1. NC_000913.3.
YP_490996.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GYP X-ray 2.10 C/D 2-446 [» ]
4IL0 X-ray 2.80 A/B/C/D/E/F/G/H 1-446 [» ]
ProteinModelPortali Q46915.
SMRi Q46915. Positions 6-445.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q46915. 3 interactions.
STRINGi 511145.b2788.

Proteomic databases

PRIDEi Q46915.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75830 ; AAC75830 ; b2788 .
BAE76860 ; BAE76860 ; BAE76860 .
GeneIDi 12931905.
947261.
KEGGi ecj:Y75_p2725.
eco:b2788.
PATRICi 32120992. VBIEscCol129921_2888.

Organism-specific databases

EchoBASEi EB2960.
EcoGenei EG13168. gudX.

Phylogenomic databases

eggNOGi COG4948.
HOGENOMi HOG000238021.
KOi K13918.
OMAi REMNHAL.
OrthoDBi EOG6V7BH9.
PhylomeDBi Q46915.

Enzyme and pathway databases

BioCyci EcoCyc:G7446-MONOMER.
ECOL316407:JW2759-MONOMER.

Miscellaneous databases

PROi Q46915.

Gene expression databases

Genevestigatori Q46915.

Family and domain databases

Gene3Di 3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProi IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view ]
PANTHERi PTHR13794. PTHR13794. 1 hit.
Pfami PF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view ]
SMARTi SM00922. MR_MLE. 1 hit.
[Graphical view ]
SUPFAMi SSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEi PS00908. MR_MLE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli."
    Hubbard B.K., Koch M., Palmer D.R., Babbitt P.C., Gerlt J.A.
    Biochemistry 37:14369-14375(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiGUDX_ECOLI
AccessioniPrimary (citable) accession number: Q46915
Secondary accession number(s): Q2MA46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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