Q468I8 (Q468I8_METBF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP MF_01897
EC=5.99.1.3 HAMAP MF_01897

Gene names

Name:	gyrA HAMAP MF_01897
Ordered Locus Names:	Mbar_A2804

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP] EMBL AAZ71704.1

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	949 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01897
Subcellular location	Cytoplasm By similarity HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_01897
Ligand	ATP-binding HAMAP MF_01897 DNA-binding HAMAP MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase HAMAP MF_01897
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q468I8 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 02E26E794A929676	FASTA	949	106,121
	10 20 30 40 50 60 MAKYEDEKKL EDELKKSYQS TLLPEEKDEV MENGDGSAAL TQEDSGKRLE LNVSDPASDD 70 80 90 100 110 120 PEDKGIEPGQ SGVTSILIEN EMKRSYINYA MSVIVGRALP DARDGFKPVH RRVLFAMKEA 130 140 150 160 170 180 GITHDKPYKK SARVVGDVLG KYHPHGDTAV YDTIVRMVQD FSLRYPLIDG QGNFGSIDGD 190 200 210 220 230 240 SAAAMRYTEV RMDRIAEEML VDIDKETVPF MPNYDGSMEE PEVLPAKLPN LLVNGSTGIA 250 260 270 280 290 300 VGMATNMAPH NIGEVIDGIL MLIENPDITI PELMTVIKGP DFPTGAHILG TAGIKSAYMT 310 320 330 340 350 360 GRGALKIRAV AEIQEFKKDR QRIIVTELPY QVNKARMIEN IAQLVREKTI MGISDLRDES 370 380 390 400 410 420 DREGIRVVIE LSRGTNPKVV LNQLYKHTQM ETTFGVINLA LVDGKPKELN LKELLEIYLE 430 440 450 460 470 480 YRMEIIQKRT LYDLRKSEER AHILDGLKIA LDNIDEVVAL IKGSANADEA KKGLMENFAL 490 500 510 520 530 540 DEIQSKAILD MRLQRLTGLE TKKVLEELEG LVKLIGELNK IIESDELKYE IIRNELLELK 550 560 570 580 590 600 EKYGDARRTK IMQAASEVRE EDLIHEEEVV VTITNGGYIK RIPLKTYTMQ HRGGRGIIGM 610 620 630 640 650 660 EMKEEDFVEN LFISSTHNYI LFFTNLGRLY WEKVYEIPEG SRQSRGKAIV NLLELKGGET 670 680 690 700 710 720 INAMIPVKEF DKNHYLLMAT REGTIKKTPL SEFKNPRKAG IIAVTLDEDD ELVRVLLTDG 730 740 750 760 770 780 KKEVLIVSKK GKAIRFSEKD VRPMGRTARG VRGMALDGPE DEVVSLDIVD ETTTLLTVTE 790 800 810 820 830 840 NGFGKRTEYS QYPAHRRGGK GVITIITNER NGPVSRVRSV ADDDELIFTS ADGIIIRIPA 850 860 870 880 890 900 KDISIQGRNT QGVRIINLKS GDKVAGIARI QNGKAEKNLK LTAFKGKEAT EATGAGEEAE 910 920 930 940 VPEGEAEDLN EFDEVEDFET ADEAEETEET EEIEGDEAEE DDVEEIEKD

References

[1]

"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ71704.1.
RefSeq	YP_306284.1. NC_007355.1.
3D structure databases
ProteinModelPortal	Q468I8.
SMR	Q468I8. Positions 95-551.
ModBase	Search...
Protein-protein interaction databases
STRING	Q468I8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3625189.
GenomeReviews	Gene locus Mbar_A2804 in contig CP000099_GR.
KEGG	mba:Mbar_A2804.
NMPDR	fig|269797.3.peg.1652.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05278.
HOGENOM	HBG635183.
OMA	TGRGRIY.
PhylomeDB	Q468I8.
ProtClustDB	PRK05560.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q468I8_METBF

Accession

Primary (citable) accession number: Q468I8

Entry history

Integrated into UniProtKB/TrEMBL:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)