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Protein

Enolase

Gene

eno

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gap1), Glyceraldehyde-3-phosphate dehydrogenase 2 (gap2)
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (gpmI1), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (gpmI2), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (Mbar_A0175)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei165SubstrateUniRule annotation1
Binding sitei174SubstrateUniRule annotation1
Active sitei217Proton donorUniRule annotation1
Metal bindingi253MagnesiumUniRule annotation1
Metal bindingi294MagnesiumUniRule annotation1
Binding sitei294SubstrateUniRule annotation1
Metal bindingi320MagnesiumUniRule annotation1
Binding sitei320SubstrateUniRule annotation1
Active sitei345Proton acceptorUniRule annotation1
Binding sitei345Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei396SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:Mbar_A2850
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000008156 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002671421 – 428EnolaseAdd BLAST428

Proteomic databases

PRIDEiQ468E2.

Interactioni

Protein-protein interaction databases

STRINGi269797.Mbar_A2850.

Structurei

3D structure databases

ProteinModelPortaliQ468E2.
SMRiQ468E2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni372 – 375Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169. Archaea.
COG0148. LUCA.
HOGENOMiHOG000072173.
KOiK01689.
OMAiEFMIIPV.
OrthoDBiPOG093Z03D9.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q468E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYIGLQQDS GEYKIQKIHA REILDSRGNP TVEVDVFTPK GFGRAGVPSG
60 70 80 90 100
ASTGTNEALE LRDADPNRYG GKGVLTAVKN VNTIIQKELL GLDVRNQREI
110 120 130 140 150
DELMIELDET DNKSNLGANA ILGVSMAVAR AAADSLNVPL YRYLGGSNAF
160 170 180 190 200
TLPVPTMNVL NGGKHAGNDL AIQEFMIQPK GAETFYEALQ IGAEIYHVLG
210 220 230 240 250
KILEKKYGRS STNVGYEGGY APKMSESTEA LDALAQAIEE AGYTDTEVTI
260 270 280 290 300
GLDAAASEFY EDEAYAIDGK KLSAPELMDY YVELVNSYPI LSIEDPFYEE
310 320 330 340 350
AFEDFEALTN ELWDTIIVGD DLFVTNIERL SKGVDMGAAN ALLLKVNQIG
360 370 380 390 400
TISEAFDAAN MASRNGYTVI VSHRSAETED TTIADISVAI GAEMIKTGAP
410 420
ARGERTAKYN QLLRIEEDLG EVAHYVQL
Length:428
Mass (Da):46,439
Last modified:September 13, 2005 - v1
Checksum:iAC99536FA3266EB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000099 Genomic DNA. Translation: AAZ71750.1.
RefSeqiWP_011307791.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ71750; AAZ71750; Mbar_A2850.
GeneIDi3627018.
KEGGimba:Mbar_A2850.

Similar proteinsi

Entry informationi

Entry nameiENO_METBF
AccessioniPrimary (citable) accession number: Q468E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 13, 2005
Last modified: September 27, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families