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Protein

CRISPR system Cascade subunit CasC

Gene

casC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).
A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasCDE alone is also able to form R-loops.

GO - Molecular functioni

  • DNA/RNA hybrid binding Source: EcoCyc
  • RNA binding Source: EcoCyc

GO - Biological processi

  • defense response to virus Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7428-MONOMER.
ECOL316407:JW2728-MONOMER.
MetaCyc:G7428-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CRISPR system Cascade subunit CasC
Gene namesi
Name:casC
Synonyms:cas4, cse4, ygcJ
Ordered Locus Names:b2758, JW2728
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13117. casC.

Subcellular locationi

GO - Cellular componenti

  • protein complex Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Loss of resistance to bacteriophage lambda infection, loss of plasmid silencing. Increased levels of 57 nucleotide crRNA and also 2 and 3 spacer-repeat units.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCurated
Chaini2 – 363362CRISPR system Cascade subunit CasCPRO_0000169322Add
BLAST

Proteomic databases

PaxDbiQ46899.

Expressioni

Inductioni

Repressed by H-NS, activated by LeuO. Activated by the BaeSR two-component regulatory system, possibly due to envelope stress. Part of the casABCDE-ygbT-ygbF operon.3 Publications

Interactioni

Subunit structurei

Part of the Cascade ribonucleoprotein complex, with stoichiometry CasA1,CasB2,CasC6,CasD1,CasE(1)-crRNA1. The 6 CasC subunits make a helical stack forming a groove in which the crRNA lies. Interacts directly with all of the Cas subunits. Stable subcomplexes of CasBCDE-crRNA and CasCDE-crRNA also form, both of which are able to bind target dsDNA, and at least CasCDE is able to form R-loops. CasCDE and CasCE complexes have endonuclease activity. Interacts with YgbT (Cas1). Binding of target ssRNA or dsDNA causes a conformational change in the Cascade complex.7 Publications

Protein-protein interaction databases

BioGridi4262275. 608 interactions.
DIPiDIP-12126N.
IntActiQ46899. 1 interaction.
STRINGi511145.b2758.

Structurei

Secondary structure

1
363
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96Combined sources
Beta strandi10 – 134Combined sources
Beta strandi15 – 184Combined sources
Beta strandi28 – 314Combined sources
Beta strandi34 – 396Combined sources
Helixi41 – 499Combined sources
Helixi52 – 576Combined sources
Beta strandi63 – 675Combined sources
Helixi68 – 769Combined sources
Turni80 – 823Combined sources
Helixi85 – 9612Combined sources
Beta strandi107 – 1148Combined sources
Helixi116 – 13217Combined sources
Helixi136 – 1449Combined sources
Helixi148 – 1514Combined sources
Helixi152 – 1554Combined sources
Helixi158 – 1636Combined sources
Beta strandi168 – 1703Combined sources
Helixi172 – 1754Combined sources
Beta strandi181 – 1844Combined sources
Beta strandi187 – 1915Combined sources
Beta strandi196 – 2027Combined sources
Helixi203 – 2064Combined sources
Beta strandi208 – 2103Combined sources
Beta strandi212 – 2165Combined sources
Beta strandi222 – 23110Combined sources
Helixi232 – 2387Combined sources
Helixi244 – 26017Combined sources
Helixi267 – 2704Combined sources
Beta strandi278 – 2869Combined sources
Helixi291 – 2944Combined sources
Beta strandi301 – 3044Combined sources
Helixi305 – 32218Combined sources
Beta strandi329 – 3335Combined sources
Beta strandi345 – 3495Combined sources
Helixi350 – 3589Combined sources
Turni359 – 3613Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QYZX-ray3.03D/E/F/G/H/I1-363[»]
4TVXX-ray3.24B/C/D/E/F/G/N/O/P/Q/R/S1-363[»]
4U7UX-ray3.00E/F/G/H/I/J/Q/R/S/T/U/V1-363[»]
5CD4X-ray3.20B/C/D/E/F/G/N/O/P/Q/R/S1-363[»]
5H9EX-ray3.21D/E/F/G/H/I1-363[»]
5H9FX-ray2.45D/E/F/G/H/I1-363[»]
ProteinModelPortaliQ46899.
SMRiQ46899. Positions 1-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG41060M0. Bacteria.
ENOG410Y6PV. LUCA.
HOGENOMiHOG000015828.
InParanoidiQ46899.
KOiK19124.
OMAiPTGKQNT.

Family and domain databases

CDDicd09646. Cas7_I-E. 1 hit.
InterProiIPR010148. CRISPR-assoc_prot_CT1975.
[Graphical view]
PfamiPF09344. Cas_CT1975. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01869. casC_Cse4. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q46899-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNFINIHVL ISHSPSCLNR DDMNMQKDAI FGGKRRVRIS SQSLKRAMRK
60 70 80 90 100
SGYYAQNIGE SSLRTIHLAQ LRDVLRQKLG ERFDQKIIDK TLALLSGKSV
110 120 130 140 150
DEAEKISADA VTPWVVGEIA WFCEQVAKAE ADNLDDKKLL KVLKEDIAAI
160 170 180 190 200
RVNLQQGVDI ALSGRMATSG MMTELGKVDG AMSIAHAITT HQVDSDIDWF
210 220 230 240 250
TAVDDLQEQG SAHLGTQEFS SGVFYRYANI NLAQLQENLG GASREQALEI
260 270 280 290 300
ATHVVHMLAT EVPGAKQRTY AAFNPADMVM VNFSDMPLSM ANAFEKAVKA
310 320 330 340 350
KDGFLQPSIQ AFNQYWDRVA NGYGLNGAAA QFSLSDVDPI TAQVKQMPTL
360
EQLKSWVRNN GEA
Length:363
Mass (Da):40,025
Last modified:November 1, 1996 - v1
Checksum:iD9A99E0CBBD2B956
GO

Mass spectrometryi

Molecular mass is 39896.3±1.3 Da from positions 2 - 363. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29579 Genomic DNA. Translation: AAA69268.1.
U00096 Genomic DNA. Translation: AAC75800.1.
AP009048 Genomic DNA. Translation: BAE76835.1.
PIRiB65057.
RefSeqiNP_417238.1. NC_000913.3.
WP_000064450.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75800; AAC75800; b2758.
BAE76835; BAE76835; BAE76835.
GeneIDi947224.
KEGGiecj:JW2728.
eco:b2758.
PATRICi32120926. VBIEscCol129921_2855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29579 Genomic DNA. Translation: AAA69268.1.
U00096 Genomic DNA. Translation: AAC75800.1.
AP009048 Genomic DNA. Translation: BAE76835.1.
PIRiB65057.
RefSeqiNP_417238.1. NC_000913.3.
WP_000064450.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QYZX-ray3.03D/E/F/G/H/I1-363[»]
4TVXX-ray3.24B/C/D/E/F/G/N/O/P/Q/R/S1-363[»]
4U7UX-ray3.00E/F/G/H/I/J/Q/R/S/T/U/V1-363[»]
5CD4X-ray3.20B/C/D/E/F/G/N/O/P/Q/R/S1-363[»]
5H9EX-ray3.21D/E/F/G/H/I1-363[»]
5H9FX-ray2.45D/E/F/G/H/I1-363[»]
ProteinModelPortaliQ46899.
SMRiQ46899. Positions 1-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262275. 608 interactions.
DIPiDIP-12126N.
IntActiQ46899. 1 interaction.
STRINGi511145.b2758.

Proteomic databases

PaxDbiQ46899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75800; AAC75800; b2758.
BAE76835; BAE76835; BAE76835.
GeneIDi947224.
KEGGiecj:JW2728.
eco:b2758.
PATRICi32120926. VBIEscCol129921_2855.

Organism-specific databases

EchoBASEiEB2918.
EcoGeneiEG13117. casC.

Phylogenomic databases

eggNOGiENOG41060M0. Bacteria.
ENOG410Y6PV. LUCA.
HOGENOMiHOG000015828.
InParanoidiQ46899.
KOiK19124.
OMAiPTGKQNT.

Enzyme and pathway databases

BioCyciEcoCyc:G7428-MONOMER.
ECOL316407:JW2728-MONOMER.
MetaCyc:G7428-MONOMER.

Miscellaneous databases

PROiQ46899.

Family and domain databases

CDDicd09646. Cas7_I-E. 1 hit.
InterProiIPR010148. CRISPR-assoc_prot_CT1975.
[Graphical view]
PfamiPF09344. Cas_CT1975. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01869. casC_Cse4. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCASC_ECOLI
AccessioniPrimary (citable) accession number: Q46899
Secondary accession number(s): Q2MA71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.