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Protein

NADPH-dependent ferric-chelate reductase

Gene

yqjH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in iron homeostasis under excess nickel conditions.1 Publication

Catalytic activityi

2 Fe(II)-siderophore + NADP+ + H+ = 2 Fe(III)-siderophore + NADPH.1 Publication

Kineticsi

  1. KM=33 µM for ferric ions (anaerobically at pH 7.5)1 Publication
  2. KM=43 µM for NADPH (anaerobically at pH 7.5)1 Publication

    GO - Molecular functioni

    • FAD binding Source: EcoCyc
    • ferric-chelate reductase (NADPH) activity Source: UniProtKB
    • flavin adenine dinucleotide binding Source: EcoCyc

    GO - Biological processi

    • cellular response to nickel ion Source: EcoCyc
    • iron assimilation Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:G7593-MONOMER.
    ECOL316407:JW3041-MONOMER.
    MetaCyc:G7593-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH-dependent ferric-chelate reductase (EC:1.16.1.9)
    Alternative name(s):
    Ferric siderophore reductase
    Gene namesi
    Name:yqjH
    Ordered Locus Names:b3070, JW3041
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12953. yqjH.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Inactivation leads to ferrous iron chelator resistance as the wild-type.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001694371 – 254NADPH-dependent ferric-chelate reductaseAdd BLAST254

    Proteomic databases

    EPDiQ46871.
    PaxDbiQ46871.
    PRIDEiQ46871.

    Expressioni

    Inductioni

    Repressed by YqjI and Fur. YqjI is required for nickel-dependent regulation of yqjH, while Fur is required for iron- and cobalt-dependent regulation of yqjH.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi4259593. 17 interactors.
    IntActiQ46871. 3 interactors.
    STRINGi511145.b3070.

    Structurei

    3D structure databases

    ProteinModelPortaliQ46871.
    SMRiQ46871.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini15 – 136FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST122

    Sequence similaritiesi

    Belongs to the SIP oxidoreductase family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiENOG4105Q26. Bacteria.
    COG2375. LUCA.
    HOGENOMiHOG000221089.
    InParanoidiQ46871.
    KOiK07229.
    OMAiVVPDIFD.
    PhylomeDBiQ46871.

    Family and domain databases

    InterProiIPR013113. FAD-bd_9_SIP.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    IPR007037. SIP.
    [Graphical view]
    PfamiPF08021. FAD_binding_9. 1 hit.
    PF04954. SIP. 1 hit.
    [Graphical view]
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46871-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNNTPRYPQR VRNDLRFREL TVLRVERISA GFQRIVLGGE ALDGFTSRGF
    60 70 80 90 100
    DDHSKLFFPQ PDAHFVPPTV TEEGIVWPEG PRPPSRDYTP LYDELRHELA
    110 120 130 140 150
    IDFFIHDGGV ASGWAMQAQP GDKLTVAGPR GSLVVPEDYA YQLYVCDESG
    160 170 180 190 200
    MPALRRRLET LSKLAVKPQV SALVSVRDNA CQDYLAHLDG FNIEWLAHDE
    210 220 230 240 250
    QAVDARLAQM QIPADDYFIW ITGEGKVVKN LSRRFEAEQY DPQRVRAAAY

    WHAK
    Length:254
    Mass (Da):28,872
    Last modified:November 1, 1996 - v1
    Checksum:i889F25AF95B78DBD
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U28379 Genomic DNA. Translation: AAA89149.1.
    U00096 Genomic DNA. Translation: AAC76105.1.
    AP009048 Genomic DNA. Translation: BAE77120.1.
    PIRiC65095.
    RefSeqiNP_417541.1. NC_000913.3.
    WP_001066494.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76105; AAC76105; b3070.
    BAE77120; BAE77120; BAE77120.
    GeneIDi947582.
    KEGGiecj:JW3041.
    eco:b3070.
    PATRICi32121556. VBIEscCol129921_3164.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U28379 Genomic DNA. Translation: AAA89149.1.
    U00096 Genomic DNA. Translation: AAC76105.1.
    AP009048 Genomic DNA. Translation: BAE77120.1.
    PIRiC65095.
    RefSeqiNP_417541.1. NC_000913.3.
    WP_001066494.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliQ46871.
    SMRiQ46871.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259593. 17 interactors.
    IntActiQ46871. 3 interactors.
    STRINGi511145.b3070.

    Proteomic databases

    EPDiQ46871.
    PaxDbiQ46871.
    PRIDEiQ46871.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76105; AAC76105; b3070.
    BAE77120; BAE77120; BAE77120.
    GeneIDi947582.
    KEGGiecj:JW3041.
    eco:b3070.
    PATRICi32121556. VBIEscCol129921_3164.

    Organism-specific databases

    EchoBASEiEB2787.
    EcoGeneiEG12953. yqjH.

    Phylogenomic databases

    eggNOGiENOG4105Q26. Bacteria.
    COG2375. LUCA.
    HOGENOMiHOG000221089.
    InParanoidiQ46871.
    KOiK07229.
    OMAiVVPDIFD.
    PhylomeDBiQ46871.

    Enzyme and pathway databases

    BioCyciEcoCyc:G7593-MONOMER.
    ECOL316407:JW3041-MONOMER.
    MetaCyc:G7593-MONOMER.

    Miscellaneous databases

    PROiQ46871.

    Family and domain databases

    InterProiIPR013113. FAD-bd_9_SIP.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    IPR007037. SIP.
    [Graphical view]
    PfamiPF08021. FAD_binding_9. 1 hit.
    PF04954. SIP. 1 hit.
    [Graphical view]
    SUPFAMiSSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiYQJH_ECOLI
    AccessioniPrimary (citable) accession number: Q46871
    Secondary accession number(s): Q2M9D6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.