Q46871 (YQJH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent ferric-chelate reductase
EC=1.16.1.9

Alternative name(s):
Ferric siderophore reductase

Gene names

Name:	yqjH
Ordered Locus Names:	b3070, JW3041

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in iron homeostasis under excess nickel conditions. Ref.4
Catalytic activity	2 Fe(II) + 2 an apo-siderophore + NADP+ + H+ = 2 an Fe(III)-siderophore + NADPH. Ref.4
Subcellular location	Cytoplasm Potential.
Induction	Repressed by YqjI and Fur. YqjI is required for nickel-dependent regulation of yqjH, while Fur is required for iron- and cobalt-dependent regulation of yqjH. Ref.4
Disruption phenotype	Inactivation leads to ferrous iron chelator resistance as the wild-type. Ref.4
Sequence similarities	Belongs to the SIP oxidoreductase family. Contains 1 FAD-binding FR-type domain.
Biophysicochemical properties	Kinetic parameters: KM=33 µM for ferric ions (anaerobically at pH 7.5) Ref.4 KM=43 µM for NADPH (anaerobically at pH 7.5)

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to nickel ion Inferred from mutant phenotype Ref.4. Source: EcoCyc iron assimilation Inferred from mutant phenotype PubMed 22098718. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	FAD binding Inferred from direct assay PubMed 22098718. Source: EcoCyc ferric-chelate reductase (NADPH) activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	NADPH-dependent ferric-chelate reductase		PRO_0000169437
Regions
	Domain	15 – 136	122	FAD-binding FR-type

Sequences

	Sequence		Length	Mass (Da)
	Q46871 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 889F25AF95B78DBD	FASTA	254	28,872
	10 20 30 40 50 60 MNNTPRYPQR VRNDLRFREL TVLRVERISA GFQRIVLGGE ALDGFTSRGF DDHSKLFFPQ 70 80 90 100 110 120 PDAHFVPPTV TEEGIVWPEG PRPPSRDYTP LYDELRHELA IDFFIHDGGV ASGWAMQAQP 130 140 150 160 170 180 GDKLTVAGPR GSLVVPEDYA YQLYVCDESG MPALRRRLET LSKLAVKPQV SALVSVRDNA 190 200 210 220 230 240 CQDYLAHLDG FNIEWLAHDE QAVDARLAQM QIPADDYFIW ITGEGKVVKN LSRRFEAEQY 250 DPQRVRAAAY WHAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Preliminary X-ray diffraction analysis of YqjH from Escherichia coli: a putative cytoplasmic ferri-siderophore reductase." Bamford V.A., Armour M., Mitchell S.A., Cartron M., Andrews S.C., Watson K.A. Acta Crystallogr. F 64:792-796(2008) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Fur and the novel regulator YqjI control transcription of the ferric reductase gene yqjH in Escherichia coli." Wang S., Wu Y., Outten F.W. J. Bacteriol. 193:563-574(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A FERRIC SIDEROPHORE REDUCTASE AND IN THE IRON HOMEOSTASIS, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28379 Genomic DNA. Translation: AAA89149.1. U00096 Genomic DNA. Translation: AAC76105.1. AP009048 Genomic DNA. Translation: BAE77120.1.
PIR	C65095.
RefSeq	NP_417541.1. NC_000913.2. YP_491261.1. NC_007779.1.
3D structure databases
ProteinModelPortal	Q46871.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q46871. 2 interactions.
STRING	511145.b3070.
Proteomic databases
PaxDb	Q46871.
PRIDE	Q46871.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76105; AAC76105; b3070. BAE77120; BAE77120; BAE77120.
GeneID	12930599. 947582.
KEGG	ecj:Y75_p2995. eco:b3070.
PATRIC	32121556. VBIEscCol129921_3164.
Organism-specific databases
EchoBASE	EB2787.
EcoGene	EG12953. yqjH.
Phylogenomic databases
eggNOG	COG2375.
HOGENOM	HOG000221089.
KO	K07229.
OMA	IDFFIHE.
ProtClustDB	CLSK880567.
Enzyme and pathway databases
BioCyc	EcoCyc:G7593-MONOMER. ECOL316407:JW3041-MONOMER. MetaCyc:G7593-MONOMER.
Gene expression databases
Genevestigator	Q46871.
Family and domain databases
InterPro	IPR013113. FAD-bd_9_SIP. IPR017927. Fd_Rdtase_FAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. IPR007037. SIP. [Graphical view]
Pfam	PF08021. FAD_binding_9. 1 hit. PF04954. SIP. 1 hit. [Graphical view]
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YQJH_ECOLI

Accession

Primary (citable) accession number: Q46871
Secondary accession number(s): Q2M9D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program