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Protein

Probable deoxycholate-binding periplasmic protein YgiS

Gene

ygiS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably part of a deoxycholate transport system. Its expression in the presence of deoxycholate in a ygiS deletion mutant increases intracellular deoxycholate levels and decreases cell growth; higher expression in the presence of deoxycholate inhibits cell growth completely. Bile acid detergents such as deoxycholate are important for host defense against bacterial growth in the gall bladder and duodenum.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YGIS-MONOMER.
ECOL316407:JW2988-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.41. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable deoxycholate-binding periplasmic protein YgiS
Gene namesi
Name:ygiS
Ordered Locus Names:b3020, JW2988
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13021. ygiS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Cells grow better in the presence of the bile acid deoxycholate. Increased membrane stability when incubated for 2 hours in the presence of 4.5% deoxycholate. Increased cell survival in the presence of 20% deoxycholate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 535515Probable deoxycholate-binding periplasmic protein YgiSPRO_0000031806Add
BLAST

Proteomic databases

PaxDbiQ46863.
PRIDEiQ46863.

Expressioni

Inductioni

Repressed by the toxin-antitoxin system MqsR-MqsA; this mRNA is degraded by mRNA interferase MqsR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259246. 6 interactions.
STRINGi511145.b3020.

Structurei

3D structure databases

ProteinModelPortaliQ46863.
SMRiQ46863. Positions 21-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108J59. Bacteria.
COG4166. LUCA.
HOGENOMiHOG000179191.
InParanoidiQ46863.
KOiK02035.
OMAiYYIELNI.
PhylomeDBiQ46863.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q46863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTRNLLWLV SLVSAAPLYA ADVPANTPLA PQQVFRYNNH SDPGTLDPQK
60 70 80 90 100
VEENTAAQIV LDLFEGLVWM DGEGQVQPAQ AERWEILDGG KRYIFHLRSG
110 120 130 140 150
LQWSDGQPLT AEDFVLGWQR AVDPKTASPF AGYLAQAHIN NAAAIVAGKA
160 170 180 190 200
DVTSLGVKAT DDRTLEVTLE QPVPWFTTML AWPTLFPVPH HVIAKHGDSW
210 220 230 240 250
SKPENMVYNG AFVLDQWVVN EKITARKNPK YRDAQHTVLQ QVEYLALDNS
260 270 280 290 300
VTGYNRYRAG EVDLTWVPAQ QIPAIEKSLP GELRIIPRLN SEYYNFNLEK
310 320 330 340 350
PPFNDVRVRR ALYLTVDRQL IAQKVLGLRT PATTLTPPEV KGFSATTFDE
360 370 380 390 400
LQKPMSERVA MAKALLKQAG YDASHPLRFE LFYNKYDLHE KTAIALSSEW
410 420 430 440 450
KKWLGAQVTL RTMEWKTYLD ARRAGDFMLS RQSWDATYND ASSFLNTLKS
460 470 480 490 500
DSEENVGHWK NAQYDALLNQ ATQITDATKR NALYQQAEVI INQQAPLIPI
510 520 530
YYQPLIKLLK PYVGGFPLHN PQDYVYSKEL YIKAH
Length:535
Mass (Da):60,694
Last modified:November 1, 1996 - v1
Checksum:iB0F56723071A54EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69188.1.
U00096 Genomic DNA. Translation: AAC76056.1.
AP009048 Genomic DNA. Translation: BAE77076.1.
PIRiB65089.
RefSeqiNP_417492.1. NC_000913.3.
WP_001295629.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76056; AAC76056; b3020.
BAE77076; BAE77076; BAE77076.
GeneIDi947140.
KEGGiecj:JW2988.
eco:b3020.
PATRICi32121454. VBIEscCol129921_3114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69188.1.
U00096 Genomic DNA. Translation: AAC76056.1.
AP009048 Genomic DNA. Translation: BAE77076.1.
PIRiB65089.
RefSeqiNP_417492.1. NC_000913.3.
WP_001295629.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ46863.
SMRiQ46863. Positions 21-535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259246. 6 interactions.
STRINGi511145.b3020.

Protein family/group databases

TCDBi3.A.1.5.41. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiQ46863.
PRIDEiQ46863.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76056; AAC76056; b3020.
BAE77076; BAE77076; BAE77076.
GeneIDi947140.
KEGGiecj:JW2988.
eco:b3020.
PATRICi32121454. VBIEscCol129921_3114.

Organism-specific databases

EchoBASEiEB2839.
EcoGeneiEG13021. ygiS.

Phylogenomic databases

eggNOGiENOG4108J59. Bacteria.
COG4166. LUCA.
HOGENOMiHOG000179191.
InParanoidiQ46863.
KOiK02035.
OMAiYYIELNI.
PhylomeDBiQ46863.

Enzyme and pathway databases

BioCyciEcoCyc:YGIS-MONOMER.
ECOL316407:JW2988-MONOMER.

Miscellaneous databases

PROiQ46863.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYGIS_ECOLI
AccessioniPrimary (citable) accession number: Q46863
Secondary accession number(s): Q2M9I0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.