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Protein

2,5-diketo-D-gluconic acid reductase A

Gene

dkgA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives.

Catalytic activityi

2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

pH dependencei

Optimum pH is 7.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei51Proton donorBy similarity1
Binding sitei107SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi187 – 241NADPBy similarityAdd BLAST55

GO - Molecular functioni

GO - Biological processi

  • L-ascorbic acid biosynthetic process Source: UniProtKB-KW
  • methylglyoxal catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ascorbate biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-148.
ECOL316407:JW5499-MONOMER.
MetaCyc:MONOMER0-148.
BRENDAi1.1.1.346. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
2,5-diketo-D-gluconic acid reductase A (EC:1.1.1.274)
Short name:
2,5-DKG reductase A
Short name:
2,5-DKGR A
Short name:
25DKGR-A
Alternative name(s):
AKR5C
Gene namesi
Name:dkgA
Synonyms:yqhE
Ordered Locus Names:b3012, JW5499
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13015. dkgA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001245991 – 2752,5-diketo-D-gluconic acid reductase AAdd BLAST275

Proteomic databases

EPDiQ46857.
PaxDbiQ46857.
PRIDEiQ46857.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262381. 5 interactors.
STRINGi511145.b3012.

Structurei

Secondary structure

1275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Beta strandi14 – 18Combined sources5
Helixi27 – 40Combined sources14
Beta strandi44 – 46Combined sources3
Helixi49 – 51Combined sources3
Helixi54 – 63Combined sources10
Helixi68 – 70Combined sources3
Beta strandi72 – 77Combined sources6
Helixi79 – 81Combined sources3
Helixi85 – 96Combined sources12
Beta strandi101 – 107Combined sources7
Turni111 – 113Combined sources3
Helixi116 – 128Combined sources13
Beta strandi131 – 139Combined sources9
Helixi142 – 152Combined sources11
Beta strandi157 – 162Combined sources6
Helixi170 – 178Combined sources9
Beta strandi182 – 187Combined sources6
Turni188 – 192Combined sources5
Turni194 – 198Combined sources5
Helixi200 – 209Combined sources10
Helixi213 – 223Combined sources11
Helixi235 – 240Combined sources6
Helixi251 – 258Combined sources8
Turni270 – 274Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MZRX-ray2.13A/B2-275[»]
ProteinModelPortaliQ46857.
SMRiQ46857.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ46857.

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiENOG4105CV8. Bacteria.
COG0656. LUCA.
HOGENOMiHOG000250272.
InParanoidiQ46857.
KOiK06221.
OMAiSENFRIF.
PhylomeDBiQ46857.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 2 hits.
[Graphical view]
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPTVIKLQ DGNVMPQLGL GVWQASNEEV ITAIQKALEV GYRSIDTAAA
60 70 80 90 100
YKNEEGVGKA LKNASVNREE LFITTKLWND DHKRPREALL DSLKKLQLDY
110 120 130 140 150
IDLYLMHWPV PAIDHYVEAW KGMIELQKEG LIKSIGVCNF QIHHLQRLID
160 170 180 190 200
ETGVTPVINQ IELHPLMQQR QLHAWNATHK IQTESWSPLA QGGKGVFDQK
210 220 230 240 250
VIRDLADKYG KTPAQIVIRW HLDSGLVVIP KSVTPSRIAE NFDVWDFRLD
260 270
KDELGEIAKL DQGKRLGPDP DQFGG
Length:275
Mass (Da):31,110
Last modified:December 15, 1998 - v3
Checksum:i0FF6E140411BAD43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69179.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76048.2.
AP009048 Genomic DNA. Translation: BAE77069.1.
PIRiB65088.
RefSeqiNP_417485.4. NC_000913.3.
WP_000013149.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76048; AAC76048; b3012.
BAE77069; BAE77069; BAE77069.
GeneIDi947495.
KEGGiecj:JW5499.
eco:b3012.
PATRICi32121440. VBIEscCol129921_3107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69179.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76048.2.
AP009048 Genomic DNA. Translation: BAE77069.1.
PIRiB65088.
RefSeqiNP_417485.4. NC_000913.3.
WP_000013149.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MZRX-ray2.13A/B2-275[»]
ProteinModelPortaliQ46857.
SMRiQ46857.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262381. 5 interactors.
STRINGi511145.b3012.

Proteomic databases

EPDiQ46857.
PaxDbiQ46857.
PRIDEiQ46857.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76048; AAC76048; b3012.
BAE77069; BAE77069; BAE77069.
GeneIDi947495.
KEGGiecj:JW5499.
eco:b3012.
PATRICi32121440. VBIEscCol129921_3107.

Organism-specific databases

EchoBASEiEB2835.
EcoGeneiEG13015. dkgA.

Phylogenomic databases

eggNOGiENOG4105CV8. Bacteria.
COG0656. LUCA.
HOGENOMiHOG000250272.
InParanoidiQ46857.
KOiK06221.
OMAiSENFRIF.
PhylomeDBiQ46857.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-148.
ECOL316407:JW5499-MONOMER.
MetaCyc:MONOMER0-148.
BRENDAi1.1.1.346. 2026.

Miscellaneous databases

EvolutionaryTraceiQ46857.
PROiQ46857.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 2 hits.
[Graphical view]
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDKGA_ECOLI
AccessioniPrimary (citable) accession number: Q46857
Secondary accession number(s): Q2M9I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.