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Q46857

- DKGA_ECOLI

UniProt

Q46857 - DKGA_ECOLI

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Protein

2,5-diketo-D-gluconic acid reductase A

Gene

dkgA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives.

Catalytic activityi

2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

pH dependencei

Optimum pH is 7.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei51 – 511Proton donorBy similarity
Binding sitei107 – 1071SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi187 – 24155NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. 2,5-didehydrogluconate reductase activity Source: UniProtKB-EC
  2. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: EcoCyc

GO - Biological processi

  1. L-ascorbic acid biosynthetic process Source: UniProtKB-KW
  2. methylglyoxal catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ascorbate biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-148.
ECOL316407:JW5499-MONOMER.
MetaCyc:MONOMER0-148.

Names & Taxonomyi

Protein namesi
Recommended name:
2,5-diketo-D-gluconic acid reductase A (EC:1.1.1.274)
Short name:
2,5-DKG reductase A
Short name:
2,5-DKGR A
Short name:
25DKGR-A
Alternative name(s):
AKR5C
Gene namesi
Name:dkgA
Synonyms:yqhE
Ordered Locus Names:b3012, JW5499
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13015. dkgA.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2752752,5-diketo-D-gluconic acid reductase APRO_0000124599Add
BLAST

Proteomic databases

PaxDbiQ46857.
PRIDEiQ46857.

Expressioni

Gene expression databases

GenevestigatoriQ46857.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi511145.b3012.

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Beta strandi14 – 185Combined sources
Helixi27 – 4014Combined sources
Beta strandi44 – 463Combined sources
Helixi49 – 513Combined sources
Helixi54 – 6310Combined sources
Helixi68 – 703Combined sources
Beta strandi72 – 776Combined sources
Helixi79 – 813Combined sources
Helixi85 – 9612Combined sources
Beta strandi101 – 1077Combined sources
Turni111 – 1133Combined sources
Helixi116 – 12813Combined sources
Beta strandi131 – 1399Combined sources
Helixi142 – 15211Combined sources
Beta strandi157 – 1626Combined sources
Helixi170 – 1789Combined sources
Beta strandi182 – 1876Combined sources
Turni188 – 1925Combined sources
Turni194 – 1985Combined sources
Helixi200 – 20910Combined sources
Helixi213 – 22311Combined sources
Helixi235 – 2406Combined sources
Helixi251 – 2588Combined sources
Turni270 – 2745Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZRX-ray2.13A/B2-275[»]
ProteinModelPortaliQ46857.
SMRiQ46857. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ46857.

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiCOG0656.
HOGENOMiHOG000250272.
InParanoidiQ46857.
KOiK06221.
OMAiPIIKLHD.
OrthoDBiEOG68H8C2.
PhylomeDBiQ46857.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46857-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANPTVIKLQ DGNVMPQLGL GVWQASNEEV ITAIQKALEV GYRSIDTAAA
60 70 80 90 100
YKNEEGVGKA LKNASVNREE LFITTKLWND DHKRPREALL DSLKKLQLDY
110 120 130 140 150
IDLYLMHWPV PAIDHYVEAW KGMIELQKEG LIKSIGVCNF QIHHLQRLID
160 170 180 190 200
ETGVTPVINQ IELHPLMQQR QLHAWNATHK IQTESWSPLA QGGKGVFDQK
210 220 230 240 250
VIRDLADKYG KTPAQIVIRW HLDSGLVVIP KSVTPSRIAE NFDVWDFRLD
260 270
KDELGEIAKL DQGKRLGPDP DQFGG
Length:275
Mass (Da):31,110
Last modified:December 15, 1998 - v3
Checksum:i0FF6E140411BAD43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69179.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76048.2.
AP009048 Genomic DNA. Translation: BAE77069.1.
PIRiB65088.
RefSeqiNP_417485.4. NC_000913.3.
YP_491205.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76048; AAC76048; b3012.
BAE77069; BAE77069; BAE77069.
GeneIDi12933387.
947495.
KEGGiecj:Y75_p2939.
eco:b3012.
PATRICi32121440. VBIEscCol129921_3107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69179.1 . Frameshift.
U00096 Genomic DNA. Translation: AAC76048.2 .
AP009048 Genomic DNA. Translation: BAE77069.1 .
PIRi B65088.
RefSeqi NP_417485.4. NC_000913.3.
YP_491205.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MZR X-ray 2.13 A/B 2-275 [» ]
ProteinModelPortali Q46857.
SMRi Q46857. Positions 2-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b3012.

Proteomic databases

PaxDbi Q46857.
PRIDEi Q46857.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76048 ; AAC76048 ; b3012 .
BAE77069 ; BAE77069 ; BAE77069 .
GeneIDi 12933387.
947495.
KEGGi ecj:Y75_p2939.
eco:b3012.
PATRICi 32121440. VBIEscCol129921_3107.

Organism-specific databases

EchoBASEi EB2835.
EcoGenei EG13015. dkgA.

Phylogenomic databases

eggNOGi COG0656.
HOGENOMi HOG000250272.
InParanoidi Q46857.
KOi K06221.
OMAi PIIKLHD.
OrthoDBi EOG68H8C2.
PhylomeDBi Q46857.

Enzyme and pathway databases

BioCyci EcoCyc:MONOMER0-148.
ECOL316407:JW5499-MONOMER.
MetaCyc:MONOMER0-148.

Miscellaneous databases

EvolutionaryTracei Q46857.
PROi Q46857.

Gene expression databases

Genevestigatori Q46857.

Family and domain databases

Gene3Di 3.20.20.100. 1 hit.
InterProi IPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view ]
PANTHERi PTHR11732. PTHR11732. 1 hit.
Pfami PF00248. Aldo_ket_red. 1 hit.
[Graphical view ]
PIRSFi PIRSF000097. AKR. 1 hit.
PRINTSi PR00069. ALDKETRDTASE.
SUPFAMi SSF51430. SSF51430. 1 hit.
PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli."
    Yum D.-Y., Lee B.-Y., Pan J.-G.
    Appl. Environ. Microbiol. 65:3341-3346(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE."
    Habrych M., Rodriguez S., Stewart J.D.
    Biotechnol. Prog. 18:257-261(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Crystal structure of Escherichia coli DkgA, a broad-specificity aldo-keto reductase."
    Jeudy S., Monchois V., Maza C., Claverie J.-M., Abergel C.
    Proteins 62:302-307(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 2-275.

Entry informationi

Entry nameiDKGA_ECOLI
AccessioniPrimary (citable) accession number: Q46857
Secondary accession number(s): Q2M9I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 26, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3