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Protein

2,5-diketo-D-gluconic acid reductase A

Gene

dkgA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives.

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Catalytic activityi

2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

pH dependencei

Optimum pH is 7.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei51Proton donorBy similarity1
Binding sitei107SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi187 – 241NADPBy similarityAdd BLAST55

GO - Molecular functioni

GO - Biological processi

  • L-ascorbic acid biosynthetic process Source: UniProtKB-KW
  • methylglyoxal catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-148
MetaCyc:MONOMER0-148
BRENDAi1.1.1.346 2026

Names & Taxonomyi

Protein namesi
Recommended name:
2,5-diketo-D-gluconic acid reductase A (EC:1.1.1.274)
Short name:
2,5-DKG reductase A
Short name:
2,5-DKGR A
Short name:
25DKGR-A
Alternative name(s):
AKR5C
Gene namesi
Name:dkgA
Synonyms:yqhE
Ordered Locus Names:b3012, JW5499
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13015 dkgA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001245991 – 2752,5-diketo-D-gluconic acid reductase AAdd BLAST275

Proteomic databases

EPDiQ46857
PaxDbiQ46857
PRIDEiQ46857

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262381, 26 interactors
STRINGi316385.ECDH10B_3187

Structurei

Secondary structure

1275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Beta strandi14 – 18Combined sources5
Helixi27 – 40Combined sources14
Beta strandi44 – 46Combined sources3
Helixi49 – 51Combined sources3
Helixi54 – 63Combined sources10
Helixi68 – 70Combined sources3
Beta strandi72 – 77Combined sources6
Helixi79 – 81Combined sources3
Helixi85 – 96Combined sources12
Beta strandi101 – 107Combined sources7
Turni111 – 113Combined sources3
Helixi116 – 128Combined sources13
Beta strandi131 – 139Combined sources9
Helixi142 – 152Combined sources11
Beta strandi157 – 162Combined sources6
Helixi170 – 178Combined sources9
Beta strandi182 – 187Combined sources6
Turni188 – 192Combined sources5
Turni194 – 198Combined sources5
Helixi200 – 209Combined sources10
Helixi213 – 223Combined sources11
Helixi235 – 240Combined sources6
Helixi251 – 258Combined sources8
Turni270 – 274Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MZRX-ray2.13A/B2-275[»]
ProteinModelPortaliQ46857
SMRiQ46857
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ46857

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiENOG4105CV8 Bacteria
COG0656 LUCA
HOGENOMiHOG000250272
InParanoidiQ46857
KOiK06221
OMAiQIELHPM
PhylomeDBiQ46857

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR018170 Aldo/ket_reductase_CS
IPR020471 Aldo/keto_reductase
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PANTHERiPTHR11732 PTHR11732, 1 hit
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 2 hits
PIRSFiPIRSF000097 AKR, 1 hit
PRINTSiPR00069 ALDKETRDTASE
SUPFAMiSSF51430 SSF51430, 1 hit
PROSITEiView protein in PROSITE
PS00798 ALDOKETO_REDUCTASE_1, 1 hit
PS00062 ALDOKETO_REDUCTASE_2, 1 hit
PS00063 ALDOKETO_REDUCTASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q46857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPTVIKLQ DGNVMPQLGL GVWQASNEEV ITAIQKALEV GYRSIDTAAA
60 70 80 90 100
YKNEEGVGKA LKNASVNREE LFITTKLWND DHKRPREALL DSLKKLQLDY
110 120 130 140 150
IDLYLMHWPV PAIDHYVEAW KGMIELQKEG LIKSIGVCNF QIHHLQRLID
160 170 180 190 200
ETGVTPVINQ IELHPLMQQR QLHAWNATHK IQTESWSPLA QGGKGVFDQK
210 220 230 240 250
VIRDLADKYG KTPAQIVIRW HLDSGLVVIP KSVTPSRIAE NFDVWDFRLD
260 270
KDELGEIAKL DQGKRLGPDP DQFGG
Length:275
Mass (Da):31,110
Last modified:December 15, 1998 - v3
Checksum:i0FF6E140411BAD43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA Translation: AAA69179.1 Frameshift.
U00096 Genomic DNA Translation: AAC76048.2
AP009048 Genomic DNA Translation: BAE77069.1
PIRiB65088
RefSeqiNP_417485.4, NC_000913.3
WP_000013149.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76048; AAC76048; b3012
BAE77069; BAE77069; BAE77069
GeneIDi947495
KEGGiecj:JW5499
eco:b3012
PATRICifig|1411691.4.peg.3717

Similar proteinsi

Entry informationi

Entry nameiDKGA_ECOLI
AccessioniPrimary (citable) accession number: Q46857
Secondary accession number(s): Q2M9I7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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