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Q46857 (DKGA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2,5-diketo-D-gluconic acid reductase A

Short name=2,5-DKG reductase A
Short name=2,5-DKGR A
Short name=25DKGR-A
EC=1.1.1.274
Alternative name(s):
AKR5C
Gene names
Name:dkgA
Synonyms:yqhE
Ordered Locus Names:b3012, JW5499
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives.

Catalytic activity

2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

Subunit structure

Monomer.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Sequence similarities

Belongs to the aldo/keto reductase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.5.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2752752,5-diketo-D-gluconic acid reductase A
PRO_0000124599

Regions

Nucleotide binding187 – 24155NADP By similarity

Sites

Active site511Proton donor By similarity
Binding site1071Substrate By similarity

Secondary structure

.................................................. 275
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q46857 [UniParc].

Last modified December 15, 1998. Version 3.
Checksum: 0FF6E140411BAD43

FASTA27531,110
        10         20         30         40         50         60 
MANPTVIKLQ DGNVMPQLGL GVWQASNEEV ITAIQKALEV GYRSIDTAAA YKNEEGVGKA 

        70         80         90        100        110        120 
LKNASVNREE LFITTKLWND DHKRPREALL DSLKKLQLDY IDLYLMHWPV PAIDHYVEAW 

       130        140        150        160        170        180 
KGMIELQKEG LIKSIGVCNF QIHHLQRLID ETGVTPVINQ IELHPLMQQR QLHAWNATHK 

       190        200        210        220        230        240 
IQTESWSPLA QGGKGVFDQK VIRDLADKYG KTPAQIVIRW HLDSGLVVIP KSVTPSRIAE 

       250        260        270 
NFDVWDFRLD KDELGEIAKL DQGKRLGPDP DQFGG 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli."
Yum D.-Y., Lee B.-Y., Pan J.-G.
Appl. Environ. Microbiol. 65:3341-3346(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE."
Habrych M., Rodriguez S., Stewart J.D.
Biotechnol. Prog. 18:257-261(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Crystal structure of Escherichia coli DkgA, a broad-specificity aldo-keto reductase."
Jeudy S., Monchois V., Maza C., Claverie J.-M., Abergel C.
Proteins 62:302-307(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 2-275.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U28377 Genomic DNA. Translation: AAA69179.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76048.2.
AP009048 Genomic DNA. Translation: BAE77069.1.
PIRB65088.
RefSeqNP_417485.4. NC_000913.3.
YP_491205.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZRX-ray2.13A/B2-275[»]
ProteinModelPortalQ46857.
SMRQ46857. Positions 2-275.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b3012.

Proteomic databases

PaxDbQ46857.
PRIDEQ46857.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76048; AAC76048; b3012.
BAE77069; BAE77069; BAE77069.
GeneID12933387.
947495.
KEGGecj:Y75_p2939.
eco:b3012.
PATRIC32121440. VBIEscCol129921_3107.

Organism-specific databases

EchoBASEEB2835.
EcoGeneEG13015. dkgA.

Phylogenomic databases

eggNOGCOG0656.
HOGENOMHOG000250272.
KOK06221.
OMAPIIKLHD.
OrthoDBEOG68H8C2.
PhylomeDBQ46857.

Enzyme and pathway databases

BioCycEcoCyc:MONOMER0-148.
ECOL316407:JW5499-MONOMER.
MetaCyc:MONOMER0-148.

Gene expression databases

GenevestigatorQ46857.

Family and domain databases

Gene3D3.20.20.100. 1 hit.
InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERPTHR11732. PTHR11732. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. SSF51430. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ46857.
PROQ46857.

Entry information

Entry nameDKGA_ECOLI
AccessionPrimary (citable) accession number: Q46857
Secondary accession number(s): Q2M9I7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: May 14, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene