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Q46857

- DKGA_ECOLI

UniProt

Q46857 - DKGA_ECOLI

Protein

2,5-diketo-D-gluconic acid reductase A

Gene

dkgA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 3 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives.

    Catalytic activityi

    2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

    pH dependencei

    Optimum pH is 7.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei51 – 511Proton donorBy similarity
    Binding sitei107 – 1071SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi187 – 24155NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 2,5-didehydrogluconate reductase activity Source: UniProtKB-EC
    2. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: EcoCyc

    GO - Biological processi

    1. L-ascorbic acid biosynthetic process Source: UniProtKB-KW
    2. methylglyoxal catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Ascorbate biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:MONOMER0-148.
    ECOL316407:JW5499-MONOMER.
    MetaCyc:MONOMER0-148.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2,5-diketo-D-gluconic acid reductase A (EC:1.1.1.274)
    Short name:
    2,5-DKG reductase A
    Short name:
    2,5-DKGR A
    Short name:
    25DKGR-A
    Alternative name(s):
    AKR5C
    Gene namesi
    Name:dkgA
    Synonyms:yqhE
    Ordered Locus Names:b3012, JW5499
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13015. dkgA.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2752752,5-diketo-D-gluconic acid reductase APRO_0000124599Add
    BLAST

    Proteomic databases

    PaxDbiQ46857.
    PRIDEiQ46857.

    Expressioni

    Gene expression databases

    GenevestigatoriQ46857.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi511145.b3012.

    Structurei

    Secondary structure

    1
    275
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 83
    Beta strandi14 – 185
    Helixi27 – 4014
    Beta strandi44 – 463
    Helixi49 – 513
    Helixi54 – 6310
    Helixi68 – 703
    Beta strandi72 – 776
    Helixi79 – 813
    Helixi85 – 9612
    Beta strandi101 – 1077
    Turni111 – 1133
    Helixi116 – 12813
    Beta strandi131 – 1399
    Helixi142 – 15211
    Beta strandi157 – 1626
    Helixi170 – 1789
    Beta strandi182 – 1876
    Turni188 – 1925
    Turni194 – 1985
    Helixi200 – 20910
    Helixi213 – 22311
    Helixi235 – 2406
    Helixi251 – 2588
    Turni270 – 2745

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MZRX-ray2.13A/B2-275[»]
    ProteinModelPortaliQ46857.
    SMRiQ46857. Positions 2-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ46857.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiCOG0656.
    HOGENOMiHOG000250272.
    KOiK06221.
    OMAiPIIKLHD.
    OrthoDBiEOG68H8C2.
    PhylomeDBiQ46857.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q46857-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANPTVIKLQ DGNVMPQLGL GVWQASNEEV ITAIQKALEV GYRSIDTAAA    50
    YKNEEGVGKA LKNASVNREE LFITTKLWND DHKRPREALL DSLKKLQLDY 100
    IDLYLMHWPV PAIDHYVEAW KGMIELQKEG LIKSIGVCNF QIHHLQRLID 150
    ETGVTPVINQ IELHPLMQQR QLHAWNATHK IQTESWSPLA QGGKGVFDQK 200
    VIRDLADKYG KTPAQIVIRW HLDSGLVVIP KSVTPSRIAE NFDVWDFRLD 250
    KDELGEIAKL DQGKRLGPDP DQFGG 275
    Length:275
    Mass (Da):31,110
    Last modified:December 15, 1998 - v3
    Checksum:i0FF6E140411BAD43
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28377 Genomic DNA. Translation: AAA69179.1. Frameshift.
    U00096 Genomic DNA. Translation: AAC76048.2.
    AP009048 Genomic DNA. Translation: BAE77069.1.
    PIRiB65088.
    RefSeqiNP_417485.4. NC_000913.3.
    YP_491205.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76048; AAC76048; b3012.
    BAE77069; BAE77069; BAE77069.
    GeneIDi12933387.
    947495.
    KEGGiecj:Y75_p2939.
    eco:b3012.
    PATRICi32121440. VBIEscCol129921_3107.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28377 Genomic DNA. Translation: AAA69179.1 . Frameshift.
    U00096 Genomic DNA. Translation: AAC76048.2 .
    AP009048 Genomic DNA. Translation: BAE77069.1 .
    PIRi B65088.
    RefSeqi NP_417485.4. NC_000913.3.
    YP_491205.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MZR X-ray 2.13 A/B 2-275 [» ]
    ProteinModelPortali Q46857.
    SMRi Q46857. Positions 2-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b3012.

    Proteomic databases

    PaxDbi Q46857.
    PRIDEi Q46857.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76048 ; AAC76048 ; b3012 .
    BAE77069 ; BAE77069 ; BAE77069 .
    GeneIDi 12933387.
    947495.
    KEGGi ecj:Y75_p2939.
    eco:b3012.
    PATRICi 32121440. VBIEscCol129921_3107.

    Organism-specific databases

    EchoBASEi EB2835.
    EcoGenei EG13015. dkgA.

    Phylogenomic databases

    eggNOGi COG0656.
    HOGENOMi HOG000250272.
    KOi K06221.
    OMAi PIIKLHD.
    OrthoDBi EOG68H8C2.
    PhylomeDBi Q46857.

    Enzyme and pathway databases

    BioCyci EcoCyc:MONOMER0-148.
    ECOL316407:JW5499-MONOMER.
    MetaCyc:MONOMER0-148.

    Miscellaneous databases

    EvolutionaryTracei Q46857.
    PROi Q46857.

    Gene expression databases

    Genevestigatori Q46857.

    Family and domain databases

    Gene3Di 3.20.20.100. 1 hit.
    InterProi IPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view ]
    PANTHERi PTHR11732. PTHR11732. 1 hit.
    Pfami PF00248. Aldo_ket_red. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000097. AKR. 1 hit.
    PRINTSi PR00069. ALDKETRDTASE.
    SUPFAMi SSF51430. SSF51430. 1 hit.
    PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli."
      Yum D.-Y., Lee B.-Y., Pan J.-G.
      Appl. Environ. Microbiol. 65:3341-3346(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE."
      Habrych M., Rodriguez S., Stewart J.D.
      Biotechnol. Prog. 18:257-261(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Crystal structure of Escherichia coli DkgA, a broad-specificity aldo-keto reductase."
      Jeudy S., Monchois V., Maza C., Claverie J.-M., Abergel C.
      Proteins 62:302-307(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 2-275.

    Entry informationi

    Entry nameiDKGA_ECOLI
    AccessioniPrimary (citable) accession number: Q46857
    Secondary accession number(s): Q2M9I7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 109 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3