Alcohol dehydrogenase YqhD - Escherichia coli (strain K12)

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Q46856 (YQHD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alcohol dehydrogenase YqhD
EC=1.1.1.-

Gene names

Name:	yqhD
Ordered Locus Names:	b3011, JW2978

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NADP-dependent ADH activity. Ref.3
Catalytic activity	An alcohol + NADP⁺ = an aldehyde + NADPH.
Cofactor	Binds 1 zinc ion per subunit.
Subunit structure	Homodimer. Ref.3
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Metal-binding NADP Zinc
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to reactive oxygen species Inferred from mutant phenotype. Source: EcoCyc
Molecular function	alcohol dehydrogenase (NADP+) activity Inferred from direct assay Ref.3. Source: EcoCyc alcohol dehydrogenase activity, zinc-dependent Inferred from direct assay Ref.3. Source: EcoCyc aldehyde dehydrogenase (NADP+) activity Inferred from direct assay. Source: EcoCyc zinc ion binding Inferred from direct assay Ref.3. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 387

387

Alcohol dehydrogenase YqhD

PRO_0000087832

Regions

Nucleotide binding

38 – 40

NADP

Nucleotide binding

93 – 99

NADP

Nucleotide binding

179 – 182

NADP

Sites

Metal binding

194

Zinc; catalytic

Metal binding

198

Zinc; catalytic

Metal binding

267

Zinc; catalytic

Metal binding

281

Zinc; catalytic

Binding site

138

NADP

Binding site

147

NADP

Binding site

160

NADP

Secondary structure

387

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q46856 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 448A7610787D2146
Show »

FASTA

387

42,097

        10         20         30         40         50         60 
MNNFNLHTPT RILFGKGAIA GLREQIPHDA RVLITYGGGS VKKTGVLDQV LDALKGMDVL 

        70         80         90        100        110        120 
EFGGIEPNPA YETLMNAVKL VREQKVTFLL AVGGGSVLDG TKFIAAAANY PENIDPWHIL 

       130        140        150        160        170        180 
QTGGKEIKSA IPMGCVLTLP ATGSESNAGA VISRKTTGDK QAFHSAHVQP VFAVLDPVYT 

       190        200        210        220        230        240 
YTLPPRQVAN GVVDAFVHTV EQYVTKPVDA KIQDRFAEGI LLTLIEDGPK ALKEPENYDV 

       250        260        270        280        290        300 
RANVMWAATQ ALNGLIGAGV PQDWATHMLG HELTAMHGLD HAQTLAIVLP ALWNEKRDTK 

       310        320        330        340        350        360 
RAKLLQYAER VWNITEGSDD ERIDAAIAAT RNFFEQLGVP THLSDYGLDG SSIPALLKKL 

       370        380 
EEHGMTQLGE NHDITLDVSR RIYEAAR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Crystal structure of E.coli alcohol dehydrogenase YqhD: evidence of a covalently modified NADP coenzyme." Sulzenbacher G., Alvarez K., Van Den Heuvel R.H., Versluis C., Spinelli S., Campanacci V., Valencia C., Cambillau C., Eklund H., Tegoni M. J. Mol. Biol. 342:489-502(2004) [PubMed: 15327949] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-387 IN COMPLEX WITH ZINC IONS AND NADP, FUNCTION, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U28377 Genomic DNA. Translation: AAA69178.1.
U00096 Genomic DNA. Translation: AAC76047.1.
AP009048 Genomic DNA. Translation: BAE77068.1.

PIR

A65088.

RefSeq

NP_417484.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OJ7	X-ray	2.00	A/B/C/D	2-387	[»]

ProteinModelPortal

Q46856.

SMR

Q46856. Positions 1-387.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-12870N.

IntAct

Q46856. 3 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004419; EBESCP00000004419; EBESCG00000003606.
EBESCT00000018108; EBESCP00000017399; EBESCG00000017164.

GeneID

947493.

GenomeReviews

Gene locus JW2978 in contig AP009048_GR.
Gene locus b3011 in contig U00096_GR.

KEGG

ecj:JW2978.
eco:b3011.

PATRIC

32121438. VBIEscCol129921_3106.

Organism-specific databases

EchoBASE

EB2834.

EcoGene

EG13014. yqhD.

Phylogenomic databases

eggNOG

COG1979.

GeneTree

EBGT00070000032043.

HOGENOM

HBG742415.

OMA

LYDIDHA.

PhylomeDB

Q46856.

ProtClustDB

PRK15138.

Enzyme and pathway databases

BioCyc

EcoCyc:G7564-MONOMER.
MetaCyc:G7564-MONOMER.

Gene expression databases

Genevestigator

Q46856.

Family and domain databases

InterPro

IPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
[Graphical view]

K08325.

Pfam

PF00465. Fe-ADH. 1 hit.
[Graphical view]

PROSITE

PS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

YQHD_ECOLI

Accession

Primary (citable) accession number: Q46856
Secondary accession number(s): Q2M9I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents