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Protein

L-glyceraldehyde 3-phosphate reductase

Gene

gpr

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the stereospecific, NADPH-dependent reduction of L-glyceraldehyde 3-phosphate (L-GAP). The physiological role of gpr is the detoxification of L-GAP, which may be formed by non-enzymatic racemization of GAP. Also involved in the stress response as a methylglyoxal reductase which converts the toxic metabolite methylglyoxal to acetol in vitro and in vivo.3 Publications

Kineticsi

  1. KM=1.06 mM for 4-nitrobenzaldehyde (at pH 7 and at 25 degrees Celsius)1 Publication
  2. KM=3.4 mM for methylglyoxal (at pH 7 and at 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei61Important for catalysis1
    Sitei66Important for catalysis1
    Sitei97Important for catalysis1
    Sitei138Important for catalysis1

    GO - Molecular functioni

    GO - Biological processi

    • cellular response to DNA damage stimulus Source: EcoliWiki
    • methylglyoxal metabolic process Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Enzyme and pathway databases

    BioCyciEcoCyc:G7558-MONOMER.
    ECOL316407:JW2970-MONOMER.
    MetaCyc:G7558-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-glyceraldehyde 3-phosphate reductase (EC:1.1.1.-)
    Short name:
    GAP reductase
    Gene namesi
    Name:gpr
    Synonyms:mgrA, yghZ
    Ordered Locus Names:b3001, JW2970
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG13010. gpr.

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene show 23% decrease in the amount of acetol.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002013311 – 346L-glyceraldehyde 3-phosphate reductaseAdd BLAST346

    Proteomic databases

    EPDiQ46851.
    PaxDbiQ46851.
    PRIDEiQ46851.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi4261416. 9 interactors.
    DIPiDIP-36026N.
    IntActiQ46851. 6 interactors.
    STRINGi511145.b3001.

    Structurei

    Secondary structure

    1346
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Turni7 – 10Combined sources4
    Beta strandi15 – 17Combined sources3
    Beta strandi24 – 31Combined sources8
    Beta strandi33 – 35Combined sources3
    Helixi42 – 54Combined sources13
    Beta strandi59 – 61Combined sources3
    Turni67 – 71Combined sources5
    Helixi72 – 84Combined sources13
    Turni86 – 88Combined sources3
    Helixi89 – 91Combined sources3
    Beta strandi93 – 98Combined sources6
    Beta strandi103 – 105Combined sources3
    Beta strandi108 – 111Combined sources4
    Helixi113 – 127Combined sources15
    Beta strandi132 – 137Combined sources6
    Helixi146 – 158Combined sources13
    Beta strandi161 – 169Combined sources9
    Helixi172 – 183Combined sources12
    Turni184 – 186Combined sources3
    Beta strandi191 – 193Combined sources3
    Helixi202 – 205Combined sources4
    Helixi208 – 215Combined sources8
    Beta strandi218 – 223Combined sources6
    Helixi226 – 231Combined sources6
    Beta strandi240 – 242Combined sources3
    Turni243 – 245Combined sources3
    Helixi261 – 276Combined sources16
    Helixi281 – 289Combined sources9
    Beta strandi296 – 300Combined sources5
    Helixi305 – 312Combined sources8
    Helixi313 – 316Combined sources4
    Helixi322 – 334Combined sources13
    Helixi341 – 344Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3N6QX-ray1.80A/B/C/D/E/F/G/H1-346[»]
    4ASTX-ray2.38A/B/C/D/E/F/G/H1-346[»]
    4AUBX-ray2.05A/B/C/D/E/F/G/H1-346[»]
    ProteinModelPortaliQ46851.
    SMRiQ46851.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CPC. Bacteria.
    COG0667. LUCA.
    HOGENOMiHOG000250283.
    InParanoidiQ46851.
    KOiK19265.
    OMAiWHSFGHV.
    PhylomeDBiQ46851.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    SUPFAMiSSF51430. SSF51430. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q46851-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVWLANPERY GQMQYRYCGK SGLRLPALSL GLWHNFGHVN ALESQRAILR
    60 70 80 90 100
    KAFDLGITHF DLANNYGPPP GSAEENFGRL LREDFAAYRD ELIISTKAGY
    110 120 130 140 150
    DMWPGPYGSG GSRKYLLASL DQSLKRMGLE YVDIFYSHRV DENTPMEETA
    160 170 180 190 200
    SALAHAVQSG KALYVGISSY SPERTQKMVE LLREWKIPLL IHQPSYNLLN
    210 220 230 240 250
    RWVDKSGLLD TLQNNGVGCI AFTPLAQGLL TGKYLNGIPQ DSRMHREGNK
    260 270 280 290 300
    VRGLTPKMLT EANLNSLRLL NEMAQQRGQS MAQMALSWLL KDDRVTSVLI
    310 320 330 340
    GASRAEQLEE NVQALNNLTF STKELAQIDQ HIADGELNLW QASSDK
    Length:346
    Mass (Da):38,832
    Last modified:November 1, 1996 - v1
    Checksum:iC70D4D43A3A57AFC
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U28377 Genomic DNA. Translation: AAA69168.1.
    U00096 Genomic DNA. Translation: AAC76037.1.
    AP009048 Genomic DNA. Translation: BAE77060.1.
    PIRiG65086.
    RefSeqiNP_417474.1. NC_000913.3.
    WP_000262172.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76037; AAC76037; b3001.
    BAE77060; BAE77060; BAE77060.
    GeneIDi947480.
    KEGGiecj:JW2970.
    eco:b3001.
    PATRICi32121420. VBIEscCol129921_3097.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U28377 Genomic DNA. Translation: AAA69168.1.
    U00096 Genomic DNA. Translation: AAC76037.1.
    AP009048 Genomic DNA. Translation: BAE77060.1.
    PIRiG65086.
    RefSeqiNP_417474.1. NC_000913.3.
    WP_000262172.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3N6QX-ray1.80A/B/C/D/E/F/G/H1-346[»]
    4ASTX-ray2.38A/B/C/D/E/F/G/H1-346[»]
    4AUBX-ray2.05A/B/C/D/E/F/G/H1-346[»]
    ProteinModelPortaliQ46851.
    SMRiQ46851.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261416. 9 interactors.
    DIPiDIP-36026N.
    IntActiQ46851. 6 interactors.
    STRINGi511145.b3001.

    Proteomic databases

    EPDiQ46851.
    PaxDbiQ46851.
    PRIDEiQ46851.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76037; AAC76037; b3001.
    BAE77060; BAE77060; BAE77060.
    GeneIDi947480.
    KEGGiecj:JW2970.
    eco:b3001.
    PATRICi32121420. VBIEscCol129921_3097.

    Organism-specific databases

    EchoBASEiEB2831.
    EcoGeneiEG13010. gpr.

    Phylogenomic databases

    eggNOGiENOG4105CPC. Bacteria.
    COG0667. LUCA.
    HOGENOMiHOG000250283.
    InParanoidiQ46851.
    KOiK19265.
    OMAiWHSFGHV.
    PhylomeDBiQ46851.

    Enzyme and pathway databases

    BioCyciEcoCyc:G7558-MONOMER.
    ECOL316407:JW2970-MONOMER.
    MetaCyc:G7558-MONOMER.

    Miscellaneous databases

    PROiQ46851.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    SUPFAMiSSF51430. SSF51430. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGPR_ECOLI
    AccessioniPrimary (citable) accession number: Q46851
    Secondary accession number(s): Q2M9J6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.