SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q46829

- BGLA_ECOLI

UniProt

Q46829 - BGLA_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
6-phospho-beta-glucosidase BglA
Gene
bglA, bglD, yqfC, b2901, JW2869
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of phosphorylated beta-glucosides into glucose-6-phosphate (G-6-P) and aglycone. It has a high affinity for phosphorylated aromatic beta-glucosides (p-nitrophenyl-beta-glucoside, phenyl beta-glucoside, arbutin), with the exception of phosphorylated salicin, and a low affinity for phosphorylated beta-methyl-glucoside. Apparently, it has only a very limited role in the utilization of external beta-glucosides.1 Publication

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei180 – 1801Proton donor Reviewed prediction
Active sitei377 – 3771Nucleophile By similarity

GO - Molecular functioni

  1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC
  2. glucosidase activity Source: EcoCyc

GO - Biological processi

  1. single-organism carbohydrate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G495-MONOMER.
ECOL316407:JW2869-MONOMER.
MetaCyc:G495-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-glucosidase BglA (EC:3.2.1.86)
Alternative name(s):
Phospho-beta-glucosidase A
Gene namesi
Name:bglA
Synonyms:bglD, yqfC
Ordered Locus Names:b2901, JW2869
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13079. bglA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4794796-phospho-beta-glucosidase BglA
PRO_0000063897Add
BLAST

Proteomic databases

PaxDbiQ46829.
PRIDEiQ46829.

Expressioni

Inductioni

Constituvely expressed.1 Publication

Gene expression databases

GenevestigatoriQ46829.

Interactioni

Subunit structurei

Homotetramer Inferred.1 Publication

Protein-protein interaction databases

DIPiDIP-9213N.
IntActiQ46829. 5 interactions.
MINTiMINT-1322539.
STRINGi511145.b2901.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 234
Turni36 – 394
Helixi62 – 654
Helixi69 – 8315
Beta strandi86 – 916
Helixi94 – 974
Beta strandi98 – 1014
Helixi108 – 12316
Beta strandi127 – 1359
Helixi139 – 1446
Helixi147 – 1493
Helixi152 – 16716
Turni168 – 1714
Beta strandi174 – 1785
Turni179 – 1824
Helixi183 – 1853
Helixi192 – 1976
Helixi201 – 2033
Beta strandi204 – 2063
Helixi207 – 23226
Beta strandi236 – 2438
Beta strandi247 – 2526
Helixi254 – 26310
Helixi265 – 27612
Helixi281 – 29010
Helixi300 – 3067
Beta strandi310 – 3156
Beta strandi320 – 3223
Beta strandi324 – 3263
Helixi355 – 36915
Beta strandi373 – 3775
Helixi395 – 41319
Beta strandi419 – 4224
Beta strandi433 – 4353
Beta strandi438 – 4414
Beta strandi444 – 4474
Beta strandi459 – 4613
Helixi463 – 47311
Turni474 – 4763

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XHYX-ray2.30A/B/C/D1-479[»]
ProteinModelPortaliQ46829.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088631.
KOiK01223.
OMAiREGCADY.
OrthoDBiEOG6F81PM.
PhylomeDBiQ46829.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46829-1 [UniParc]FASTAAdd to Basket

« Hide

MIVKKLTLPK DFLWGGAVAA HQVEGGWNKG GKGPSICDVL TGGAHGVPRE    50
ITKEVLPGKY YPNHEAVDFY GHYKEDIKLF AEMGFKCFRT SIAWTRIFPK 100
GDEAQPNEEG LKFYDDMFDE LLKYNIEPVI TLSHFEMPLH LVQQYGSWTN 150
RKVVDFFVRF AEVVFERYKH KVKYWMTFNE INNQRNWRAP LFGYCCSGVV 200
YTEHENPEET MYQVLHHQFV ASALAVKAAR RINPEMKVGC MLAMVPLYPY 250
SCNPDDVMFA QESMRERYVF TDVQLRGYYP SYVLNEWERR GFNIKMEDGD 300
LDVLREGTCD YLGFSYYMTN AVKAEGGTGD AISGFEGSVP NPYVKASDWG 350
WQIDPVGLRY ALCELYERYQ RPLFIVENGF GAYDKVEEDG SINDDYRIDY 400
LRAHIEEMKK AVTYDGVDLM GYTPWGCIDC VSFTTGQYSK RYGFIYVNKH 450
DDGTGDMSRS RKKSFNWYKE VIASNGEKL 479
Length:479
Mass (Da):55,361
Last modified:November 1, 1997 - v2
Checksum:iAA493EDAB0E95A56
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28375 Genomic DNA. Translation: AAA83082.1.
U00096 Genomic DNA. Translation: AAC75939.1.
AP009048 Genomic DNA. Translation: BAE76966.1.
PIRiE65074.
RefSeqiNP_417377.1. NC_000913.3.
YP_491102.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75939; AAC75939; b2901.
BAE76966; BAE76966; BAE76966.
GeneIDi12934286.
947378.
KEGGiecj:Y75_p2833.
eco:b2901.
PATRICi32121216. VBIEscCol129921_2996.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28375 Genomic DNA. Translation: AAA83082.1 .
U00096 Genomic DNA. Translation: AAC75939.1 .
AP009048 Genomic DNA. Translation: BAE76966.1 .
PIRi E65074.
RefSeqi NP_417377.1. NC_000913.3.
YP_491102.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2XHY X-ray 2.30 A/B/C/D 1-479 [» ]
ProteinModelPortali Q46829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9213N.
IntActi Q46829. 5 interactions.
MINTi MINT-1322539.
STRINGi 511145.b2901.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi Q46829.
PRIDEi Q46829.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75939 ; AAC75939 ; b2901 .
BAE76966 ; BAE76966 ; BAE76966 .
GeneIDi 12934286.
947378.
KEGGi ecj:Y75_p2833.
eco:b2901.
PATRICi 32121216. VBIEscCol129921_2996.

Organism-specific databases

EchoBASEi EB2889.
EcoGenei EG13079. bglA.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088631.
KOi K01223.
OMAi REGCADY.
OrthoDBi EOG6F81PM.
PhylomeDBi Q46829.

Enzyme and pathway databases

BioCyci EcoCyc:G495-MONOMER.
ECOL316407:JW2869-MONOMER.
MetaCyc:G495-MONOMER.

Miscellaneous databases

PROi Q46829.

Gene expression databases

Genevestigatori Q46829.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Genetic determination of the constitutive biosynthesis of phospho--glucosidase A in Escherichia coli K-12."
    Prasad I., Young B., Schaefler S.
    J. Bacteriol. 114:909-915(1973) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A GLUCOSIDASE, SUBSTRATE SPECIFICITY, INDUCTION.
  4. "Macro-to-micro structural proteomics: native source proteins for high-throughput crystallization."
    Totir M., Echols N., Nanao M., Gee C.L., Moskaleva A., Gradia S., Iavarone A.T., Berger J.M., May A.P., Zubieta C., Alber T.
    PLoS ONE 7:E32498-E32498(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiBGLA_ECOLI
AccessioniPrimary (citable) accession number: Q46829
Secondary accession number(s): Q2M9U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi