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Protein

6-phospho-beta-glucosidase BglA

Gene

bglA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of phosphorylated beta-glucosides into glucose-6-phosphate (G-6-P) and aglycone. It has a high affinity for phosphorylated aromatic beta-glucosides (p-nitrophenyl-beta-glucoside, phenyl beta-glucoside, arbutin), with the exception of phosphorylated salicin, and a low affinity for phosphorylated beta-methyl-glucoside. Apparently, it has only a very limited role in the utilization of external beta-glucosides.1 Publication

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei180Proton donorSequence analysis1
Active sitei377NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G495-MONOMER.
ECOL316407:JW2869-MONOMER.
MetaCyc:G495-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-glucosidase BglA (EC:3.2.1.86)
Alternative name(s):
Phospho-beta-glucosidase A
Gene namesi
Name:bglA
Synonyms:bglD, yqfC
Ordered Locus Names:b2901, JW2869
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13079. bglA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638971 – 4796-phospho-beta-glucosidase BglAAdd BLAST479

Proteomic databases

PaxDbiQ46829.
PRIDEiQ46829.

Expressioni

Inductioni

Constituvely expressed.1 Publication

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi4263280. 18 interactors.
DIPiDIP-9213N.
IntActiQ46829. 5 interactors.
MINTiMINT-1322539.
STRINGi511145.b2901.

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 23Combined sources4
Turni36 – 39Combined sources4
Helixi62 – 65Combined sources4
Helixi69 – 83Combined sources15
Beta strandi86 – 91Combined sources6
Helixi94 – 97Combined sources4
Beta strandi98 – 101Combined sources4
Helixi108 – 123Combined sources16
Beta strandi127 – 135Combined sources9
Helixi139 – 144Combined sources6
Helixi147 – 149Combined sources3
Helixi152 – 167Combined sources16
Turni168 – 171Combined sources4
Beta strandi174 – 178Combined sources5
Turni179 – 182Combined sources4
Helixi183 – 185Combined sources3
Helixi192 – 197Combined sources6
Helixi201 – 203Combined sources3
Beta strandi204 – 206Combined sources3
Helixi207 – 232Combined sources26
Beta strandi236 – 243Combined sources8
Beta strandi247 – 252Combined sources6
Helixi254 – 263Combined sources10
Helixi265 – 276Combined sources12
Helixi281 – 290Combined sources10
Helixi300 – 306Combined sources7
Beta strandi310 – 315Combined sources6
Beta strandi320 – 322Combined sources3
Beta strandi324 – 326Combined sources3
Helixi355 – 369Combined sources15
Beta strandi373 – 377Combined sources5
Helixi395 – 413Combined sources19
Beta strandi419 – 422Combined sources4
Beta strandi433 – 435Combined sources3
Beta strandi438 – 441Combined sources4
Beta strandi444 – 447Combined sources4
Beta strandi459 – 461Combined sources3
Helixi463 – 473Combined sources11
Turni474 – 476Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XHYX-ray2.30A/B/C/D1-479[»]
ProteinModelPortaliQ46829.
SMRiQ46829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Phylogenomic databases

eggNOGiENOG4105CS2. Bacteria.
COG2723. LUCA.
HOGENOMiHOG000088631.
InParanoidiQ46829.
KOiK01223.
OMAiMRGYYPA.
PhylomeDBiQ46829.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVKKLTLPK DFLWGGAVAA HQVEGGWNKG GKGPSICDVL TGGAHGVPRE
60 70 80 90 100
ITKEVLPGKY YPNHEAVDFY GHYKEDIKLF AEMGFKCFRT SIAWTRIFPK
110 120 130 140 150
GDEAQPNEEG LKFYDDMFDE LLKYNIEPVI TLSHFEMPLH LVQQYGSWTN
160 170 180 190 200
RKVVDFFVRF AEVVFERYKH KVKYWMTFNE INNQRNWRAP LFGYCCSGVV
210 220 230 240 250
YTEHENPEET MYQVLHHQFV ASALAVKAAR RINPEMKVGC MLAMVPLYPY
260 270 280 290 300
SCNPDDVMFA QESMRERYVF TDVQLRGYYP SYVLNEWERR GFNIKMEDGD
310 320 330 340 350
LDVLREGTCD YLGFSYYMTN AVKAEGGTGD AISGFEGSVP NPYVKASDWG
360 370 380 390 400
WQIDPVGLRY ALCELYERYQ RPLFIVENGF GAYDKVEEDG SINDDYRIDY
410 420 430 440 450
LRAHIEEMKK AVTYDGVDLM GYTPWGCIDC VSFTTGQYSK RYGFIYVNKH
460 470
DDGTGDMSRS RKKSFNWYKE VIASNGEKL
Length:479
Mass (Da):55,361
Last modified:November 1, 1997 - v2
Checksum:iAA493EDAB0E95A56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83082.1.
U00096 Genomic DNA. Translation: AAC75939.1.
AP009048 Genomic DNA. Translation: BAE76966.1.
PIRiE65074.
RefSeqiNP_417377.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75939; AAC75939; b2901.
BAE76966; BAE76966; BAE76966.
GeneIDi947378.
KEGGiecj:JW2869.
eco:b2901.
PATRICi32121216. VBIEscCol129921_2996.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83082.1.
U00096 Genomic DNA. Translation: AAC75939.1.
AP009048 Genomic DNA. Translation: BAE76966.1.
PIRiE65074.
RefSeqiNP_417377.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XHYX-ray2.30A/B/C/D1-479[»]
ProteinModelPortaliQ46829.
SMRiQ46829.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263280. 18 interactors.
DIPiDIP-9213N.
IntActiQ46829. 5 interactors.
MINTiMINT-1322539.
STRINGi511145.b2901.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ46829.
PRIDEiQ46829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75939; AAC75939; b2901.
BAE76966; BAE76966; BAE76966.
GeneIDi947378.
KEGGiecj:JW2869.
eco:b2901.
PATRICi32121216. VBIEscCol129921_2996.

Organism-specific databases

EchoBASEiEB2889.
EcoGeneiEG13079. bglA.

Phylogenomic databases

eggNOGiENOG4105CS2. Bacteria.
COG2723. LUCA.
HOGENOMiHOG000088631.
InParanoidiQ46829.
KOiK01223.
OMAiMRGYYPA.
PhylomeDBiQ46829.

Enzyme and pathway databases

BioCyciEcoCyc:G495-MONOMER.
ECOL316407:JW2869-MONOMER.
MetaCyc:G495-MONOMER.

Miscellaneous databases

PROiQ46829.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGLA_ECOLI
AccessioniPrimary (citable) accession number: Q46829
Secondary accession number(s): Q2M9U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.