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Protein

Toxin CptA

Gene

cptA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Overexpression leads to growth arrest after 5 hours, with initial elongation of cells, followed by swelling. The toxic effects are abrogated by coexpression with antitoxin CptB. Interacts with cytoskeletal proteins FtsZ and MreB; inhibits FtsZ GTP-dependent polymerization as well as MreB ATP-dependent polymerization.1 Publication

GO - Biological processi

  • negative regulation of protein binding Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Cell shape

Enzyme and pathway databases

BioCyciEcoCyc:G7509-MONOMER.
ECOL316407:JW2864-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin CptA
Gene namesi
Name:cptA
Synonyms:ygfX
Ordered Locus Names:b2896, JW2864
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13074. cptA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3221HelicalSequence analysisAdd
BLAST
Topological domaini33 – 375PeriplasmicSequence analysis
Transmembranei38 – 5417HelicalSequence analysisAdd
BLAST
Topological domaini55 – 13581CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 135135Toxin CptAPRO_0000169359Add
BLAST

Proteomic databases

PaxDbiQ46824.
PRIDEiQ46824.

Interactioni

Subunit structurei

Interacts with MreB and FtsZ; interaction with the latter requires FtsZ residues 33-49.1 Publication

Protein-protein interaction databases

BioGridi4262341. 9 interactions.
IntActiQ46824. 1 interaction.
STRINGi511145.b2896.

Structurei

3D structure databases

ProteinModelPortaliQ46824.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9696Not required to inhibit FtsZ or MreB polymerizationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108YDB. Bacteria.
ENOG4111R98. LUCA.
HOGENOMiHOG000124380.
KOiK19168.
OMAiWLASDSM.
OrthoDBiEOG6XDGXD.

Family and domain databases

InterProiIPR009883. Cpta_toxin.
[Graphical view]
PfamiPF07254. Cpta_toxin. 1 hit.
[Graphical view]
PIRSFiPIRSF020653. UCP020653. 1 hit.
ProDomiPD083358. DUF1434. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q46824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLWQSDLRV SWRAQWLSLL IHGLVAAVIL LMPWPLSYTP LWMVLLSLVV
60 70 80 90 100
FDCVRSQRRI NARQGEIRLL MDGRLRWQGQ EWSIVKAPWM IKSGMMLRLR
110 120 130
SDGGKRQHLW LAADSMDEAE WRDLRRILLQ QETQR
Length:135
Mass (Da):16,064
Last modified:November 1, 1996 - v1
Checksum:iCB3D38F10AAD1D98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83077.1.
U00096 Genomic DNA. Translation: AAC75934.1.
AP009048 Genomic DNA. Translation: BAE76961.1.
PIRiH65073.
RefSeqiNP_417372.1. NC_000913.3.
WP_000244777.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75934; AAC75934; b2896.
BAE76961; BAE76961; BAE76961.
GeneIDi947379.
KEGGiecj:JW2864.
eco:b2896.
PATRICi32121204. VBIEscCol129921_2990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83077.1.
U00096 Genomic DNA. Translation: AAC75934.1.
AP009048 Genomic DNA. Translation: BAE76961.1.
PIRiH65073.
RefSeqiNP_417372.1. NC_000913.3.
WP_000244777.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ46824.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262341. 9 interactions.
IntActiQ46824. 1 interaction.
STRINGi511145.b2896.

Proteomic databases

PaxDbiQ46824.
PRIDEiQ46824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75934; AAC75934; b2896.
BAE76961; BAE76961; BAE76961.
GeneIDi947379.
KEGGiecj:JW2864.
eco:b2896.
PATRICi32121204. VBIEscCol129921_2990.

Organism-specific databases

EchoBASEiEB2884.
EcoGeneiEG13074. cptA.

Phylogenomic databases

eggNOGiENOG4108YDB. Bacteria.
ENOG4111R98. LUCA.
HOGENOMiHOG000124380.
KOiK19168.
OMAiWLASDSM.
OrthoDBiEOG6XDGXD.

Enzyme and pathway databases

BioCyciEcoCyc:G7509-MONOMER.
ECOL316407:JW2864-MONOMER.

Miscellaneous databases

PROiQ46824.

Family and domain databases

InterProiIPR009883. Cpta_toxin.
[Graphical view]
PfamiPF07254. Cpta_toxin. 1 hit.
[Graphical view]
PIRSFiPIRSF020653. UCP020653. 1 hit.
ProDomiPD083358. DUF1434. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "A novel membrane-bound toxin for cell division, CptA (YgfX), inhibits polymerization of cytoskeleton proteins, FtsZ and MreB, in Escherichia coli."
    Masuda H., Tan Q., Awano N., Yamaguchi Y., Inouye M.
    FEMS Microbiol. Lett. 328:174-181(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TOXIN, SUBCELLULAR LOCATION, INTERACTION WITH FTSZ AND MREB.
    Strain: B / BL21-DE3 and K12 / BW25113.

Entry informationi

Entry nameiCPTA_ECOLI
AccessioniPrimary (citable) accession number: Q46824
Secondary accession number(s): Q2M9U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: January 20, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.