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Protein

Inner membrane protein YgfX

Gene

ygfX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A probable inner membrane protein. Has been shown not to be a toxin, no effects on growth are seen in LB or minimal medium up to 6 or 21 hours (respectively) after induction of expression (PubMed:23657679). Interacts with cytoskeletal proteins FtsZ and MreB; inhibits FtsZ GTP-dependent polymerization as well as MreB ATP-dependent polymerization (PubMed:22239607). Restores production of prodigiosin antibiotic (Pig) in Serratia strains with deletions of sdhE-ygfX; overexpression of this protein and CptB also restores Pig production to a slightly lesser extent in Serratia (PubMed:22474332).3 Publications

Caution

Was originally thought to be the toxic component of a type II toxin-antitoxin (TA) system; overexpression was shown to lead to growth arrest after 5 hours, with initial elongation of cells, followed by swelling (PubMed:22239607). The toxic effects were abrogated by coexpression with antitoxin CptB (now sdhE) (PubMed:22239607). However subsequent studies have been unable to show the toxic effect upon overexpression, nor is the Serratia ortholog a toxin (PubMed:23657679).2 Publications

GO - Biological processi

  • negative regulation of protein binding Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Biological processCell shape

Enzyme and pathway databases

BioCyciEcoCyc:G7509-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Inner membrane protein YgfX
Alternative name(s):
Toxin CptA1 Publication
Gene namesi
Name:ygfX1 Publication
Synonyms:cptA1 Publication
Ordered Locus Names:b2896, JW2864
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13074 cptA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 37PeriplasmicSequence analysis5
Transmembranei38 – 54HelicalSequence analysisAdd BLAST17
Topological domaini55 – 135CytoplasmicSequence analysisAdd BLAST81

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001693591 – 135Inner membrane protein YgfXAdd BLAST135

Proteomic databases

PaxDbiQ46824
PRIDEiQ46824

Interactioni

Subunit structurei

Interacts with MreB and FtsZ; interaction with the latter requires FtsZ residues 33-49 (PubMed:22239607).1 Publication

Protein-protein interaction databases

BioGridi4262341, 9 interactors
IntActiQ46824, 1 interactor
STRINGi316385.ECDH10B_3070

Structurei

3D structure databases

ProteinModelPortaliQ46824
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 96Not required to inhibit FtsZ or MreB polymerizationAdd BLAST96

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108YDB Bacteria
ENOG4111R98 LUCA
HOGENOMiHOG000124380
KOiK19168
OMAiWLASDSM

Family and domain databases

InterProiView protein in InterPro
IPR009883 YgfX
PfamiView protein in Pfam
PF07254 Cpta_toxin, 1 hit
PIRSFiPIRSF020653 UCP020653, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD083358 DUF1434, 1 hit

Sequencei

Sequence statusi: Complete.

Q46824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLWQSDLRV SWRAQWLSLL IHGLVAAVIL LMPWPLSYTP LWMVLLSLVV
60 70 80 90 100
FDCVRSQRRI NARQGEIRLL MDGRLRWQGQ EWSIVKAPWM IKSGMMLRLR
110 120 130
SDGGKRQHLW LAADSMDEAE WRDLRRILLQ QETQR
Length:135
Mass (Da):16,064
Last modified:November 1, 1996 - v1
Checksum:iCB3D38F10AAD1D98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83077.1
U00096 Genomic DNA Translation: AAC75934.1
AP009048 Genomic DNA Translation: BAE76961.1
PIRiH65073
RefSeqiNP_417372.1, NC_000913.3
WP_000244777.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75934; AAC75934; b2896
BAE76961; BAE76961; BAE76961
GeneIDi947379
KEGGiecj:JW2864
eco:b2896
PATRICifig|1411691.4.peg.3837

Similar proteinsi

Entry informationi

Entry nameiYGFX_ECOLI
AccessioniPrimary (citable) accession number: Q46824
Secondary accession number(s): Q2M9U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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