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Protein

Uric acid transporter UacT

Gene

uacT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-dependent high-capacity transporter for uric acid. Shows also a low capacity for transport of xanthine at 37 degrees Celsius but not at 25 degrees Celsius.1 Publication

Enzyme regulationi

Inhibited in the presence of the protonophore carbonyl cyanide m-chlorophenyl hydrazone.1 Publication

Kineticsi

  1. KM=0.5 mM for uric acid1 Publication
  2. KM=0.3 mM for xanthine1 Publication
  1. Vmax=715 nmol/min/mg enzyme with acid uric as substrate1 Publication
  2. Vmax=14 nmol/min/mg enzyme with xanthine as substrate (at 37 degrees Celsius)1 Publication

GO - Molecular functioni

  • symporter activity Source: UniProtKB-KW
  • urate transmembrane transporter activity Source: EcoCyc
  • xanthine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • proton transport Source: UniProtKB-KW
  • urate transport Source: EcoCyc
  • xanthine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YGFU-MONOMER.
ECOL316407:JW5470-MONOMER.
MetaCyc:YGFU-MONOMER.

Protein family/group databases

TCDBi2.A.40.3.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Uric acid transporter UacT
Gene namesi
Name:uacT
Synonyms:ygfU
Ordered Locus Names:b2888, JW5470
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13071. uacT.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Topological domaini51 – 62PeriplasmicSequence analysisAdd BLAST12
Transmembranei63 – 83HelicalSequence analysisAdd BLAST21
Topological domaini84 – 92CytoplasmicSequence analysis9
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 115PeriplasmicSequence analysis2
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 142CytoplasmicSequence analysis6
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Topological domaini164 – 178PeriplasmicSequence analysisAdd BLAST15
Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
Topological domaini200 – 204CytoplasmicSequence analysis5
Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
Topological domaini226 – 261PeriplasmicSequence analysisAdd BLAST36
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 337CytoplasmicSequence analysisAdd BLAST55
Transmembranei338 – 358HelicalSequence analysisAdd BLAST21
Topological domaini359PeriplasmicSequence analysis1
Transmembranei360 – 380HelicalSequence analysisAdd BLAST21
Topological domaini381 – 392CytoplasmicSequence analysisAdd BLAST12
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 421PeriplasmicSequence analysis8
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21
Topological domaini443 – 482CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi37H → K or L: Lack of activity. 1 Publication1
Mutagenesisi37H → N: Strong decrease in activity. 1 Publication1
Mutagenesisi100T → A: Decrease in activity with uric acid. Highly active with xanthine. 1 Publication1
Mutagenesisi100T → S: Decrease in activity. 1 Publication1
Mutagenesisi256S → Q: Almost no change in activity. 1 Publication1
Mutagenesisi259T → V: Almost no change in activity. 1 Publication1
Mutagenesisi270E → D, N or Q: Lack of activity. 1 Publication1
Mutagenesisi278L → T: Almost no change in activity. 1 Publication1
Mutagenesisi298D → E: Strong decrease in activity. 1 Publication1
Mutagenesisi298D → N: Lack of activity. 1 Publication1
Mutagenesisi317S → A: Decrease in activity. 1 Publication1
Mutagenesisi318Q → E or N: Strong decrease in activity. 1 Publication1
Mutagenesisi319N → D or Q: Lack of activity. 1 Publication1
Mutagenesisi320V → N: Lack of activity. 1 Publication1
Mutagenesisi327R → G: Almost no change in activity. 1 Publication1
Mutagenesisi426S → N: Almost no change in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659681 – 482Uric acid transporter UacTAdd BLAST482

Proteomic databases

PaxDbiQ46821.
PRIDEiQ46821.

Interactioni

Protein-protein interaction databases

BioGridi4262336. 160 interactors.
DIPiDIP-12176N.
STRINGi511145.b2888.

Structurei

3D structure databases

ProteinModelPortaliQ46821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038199.
InParanoidiQ46821.
OMAiFYFGVPE.
PhylomeDBiQ46821.

Family and domain databases

InterProiIPR017588. UacT-like.
IPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
TIGR03173. pbuX. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIDSQLPS SSGQDRPTDE VDRILSPGKL IILGLQHVLV MYAGAVAVPL
60 70 80 90 100
MIGDRLGLSK EAIAMLISSD LFCCGIVTLL QCIGIGRFMG IRLPVIMSVT
110 120 130 140 150
FAAVTPMIAI GMNPDIGLLG IFGATIAAGF ITTLLAPLIG RLMPLFPPLV
160 170 180 190 200
TGVVITSIGL SIIQVGIDWA AGGKGNPQYG NPVYLGISFA VLIFILLITR
210 220 230 240 250
YAKGFMSNVA VLLGIVFGFL LSWMMNEVNL SGLHDASWFA IVTPMSFGMP
260 270 280 290 300
IFDPVSILTM TAVLIIVFIE SMGMFLALGE IVGRKLSSHD IIRGLRVDGV
310 320 330 340 350
GTMIGGTFNS FPHTSFSQNV GLVSVTRVHS RWVCISSGII LILFGMVPKM
360 370 380 390 400
AVLVASIPQF VLGGAGLVMF GMVLATGIRI LSRCNYTTNR YNLYIVAISL
410 420 430 440 450
GVGMTPTLSH DFFSKLPAVL QPLLHSGIML ATLSAVVLNV FFNGYQHHAD
460 470 480
LVKESVSDKD LKVRTVRMWL LMRKLKKNEH GE
Length:482
Mass (Da):51,758
Last modified:May 30, 2000 - v2
Checksum:iE08EF1D4CBD9D066
GO

Sequence cautioni

The sequence AAA83069 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83069.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75926.2.
AP009048 Genomic DNA. Translation: BAE76953.1.
PIRiH65072.
RefSeqiNP_417364.2. NC_000913.3.
WP_001295374.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75926; AAC75926; b2888.
BAE76953; BAE76953; BAE76953.
GeneIDi949017.
KEGGiecj:JW5470.
eco:b2888.
PATRICi32121184. VBIEscCol129921_2980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83069.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75926.2.
AP009048 Genomic DNA. Translation: BAE76953.1.
PIRiH65072.
RefSeqiNP_417364.2. NC_000913.3.
WP_001295374.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ46821.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262336. 160 interactors.
DIPiDIP-12176N.
STRINGi511145.b2888.

Protein family/group databases

TCDBi2.A.40.3.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiQ46821.
PRIDEiQ46821.

Protocols and materials databases

DNASUi949017.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75926; AAC75926; b2888.
BAE76953; BAE76953; BAE76953.
GeneIDi949017.
KEGGiecj:JW5470.
eco:b2888.
PATRICi32121184. VBIEscCol129921_2980.

Organism-specific databases

EchoBASEiEB2882.
EcoGeneiEG13071. uacT.

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038199.
InParanoidiQ46821.
OMAiFYFGVPE.
PhylomeDBiQ46821.

Enzyme and pathway databases

BioCyciEcoCyc:YGFU-MONOMER.
ECOL316407:JW5470-MONOMER.
MetaCyc:YGFU-MONOMER.

Miscellaneous databases

PROiQ46821.

Family and domain databases

InterProiIPR017588. UacT-like.
IPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
TIGR03173. pbuX. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUACT_ECOLI
AccessioniPrimary (citable) accession number: Q46821
Secondary accession number(s): Q2M9V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.