Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Uric acid transporter UacT

Gene

uacT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proton-dependent high-capacity transporter for uric acid. Shows also a low capacity for transport of xanthine at 37 degrees Celsius but not at 25 degrees Celsius.1 Publication

Enzyme regulationi

Inhibited in the presence of the protonophore carbonyl cyanide m-chlorophenyl hydrazone.1 Publication

Kineticsi

  1. KM=0.5 mM for uric acid1 Publication
  2. KM=0.3 mM for xanthine1 Publication
  1. Vmax=715 nmol/min/mg enzyme with acid uric as substrate1 Publication
  2. Vmax=14 nmol/min/mg enzyme with xanthine as substrate (at 37 degrees Celsius)1 Publication

GO - Molecular functioni

  • symporter activity Source: UniProtKB-KW
  • urate transmembrane transporter activity Source: EcoCyc
  • xanthine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • urate transport Source: EcoCyc
  • xanthine transport Source: EcoCyc

Keywordsi

Biological processHydrogen ion transport, Ion transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YGFU-MONOMER
MetaCyc:YGFU-MONOMER

Protein family/group databases

TCDBi2.A.40.3.3 the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family

Names & Taxonomyi

Protein namesi
Recommended name:
Uric acid transporter UacT
Gene namesi
Name:uacT
Synonyms:ygfU
Ordered Locus Names:b2888, JW5470
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13071 uacT

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Topological domaini51 – 62PeriplasmicSequence analysisAdd BLAST12
Transmembranei63 – 83HelicalSequence analysisAdd BLAST21
Topological domaini84 – 92CytoplasmicSequence analysis9
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 115PeriplasmicSequence analysis2
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 142CytoplasmicSequence analysis6
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Topological domaini164 – 178PeriplasmicSequence analysisAdd BLAST15
Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
Topological domaini200 – 204CytoplasmicSequence analysis5
Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
Topological domaini226 – 261PeriplasmicSequence analysisAdd BLAST36
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 337CytoplasmicSequence analysisAdd BLAST55
Transmembranei338 – 358HelicalSequence analysisAdd BLAST21
Topological domaini359PeriplasmicSequence analysis1
Transmembranei360 – 380HelicalSequence analysisAdd BLAST21
Topological domaini381 – 392CytoplasmicSequence analysisAdd BLAST12
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 421PeriplasmicSequence analysis8
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21
Topological domaini443 – 482CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi37H → K or L: Lack of activity. 1 Publication1
Mutagenesisi37H → N: Strong decrease in activity. 1 Publication1
Mutagenesisi100T → A: Decrease in activity with uric acid. Highly active with xanthine. 1 Publication1
Mutagenesisi100T → S: Decrease in activity. 1 Publication1
Mutagenesisi256S → Q: Almost no change in activity. 1 Publication1
Mutagenesisi259T → V: Almost no change in activity. 1 Publication1
Mutagenesisi270E → D, N or Q: Lack of activity. 1 Publication1
Mutagenesisi278L → T: Almost no change in activity. 1 Publication1
Mutagenesisi298D → E: Strong decrease in activity. 1 Publication1
Mutagenesisi298D → N: Lack of activity. 1 Publication1
Mutagenesisi317S → A: Decrease in activity. 1 Publication1
Mutagenesisi318Q → E or N: Strong decrease in activity. 1 Publication1
Mutagenesisi319N → D or Q: Lack of activity. 1 Publication1
Mutagenesisi320V → N: Lack of activity. 1 Publication1
Mutagenesisi327R → G: Almost no change in activity. 1 Publication1
Mutagenesisi426S → N: Almost no change in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659681 – 482Uric acid transporter UacTAdd BLAST482

Proteomic databases

PaxDbiQ46821
PRIDEiQ46821

Interactioni

Protein-protein interaction databases

BioGridi4262336, 163 interactors
DIPiDIP-12176N
STRINGi316385.ECDH10B_3062

Structurei

3D structure databases

ProteinModelPortaliQ46821
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W Bacteria
COG2233 LUCA
HOGENOMiHOG000038199
InParanoidiQ46821
OMAiFTRRDRF
PhylomeDBiQ46821

Family and domain databases

InterProiView protein in InterPro
IPR017588 UacT-like
IPR006042 Xan_ur_permease
IPR006043 Xant/urac/vitC
PfamiView protein in Pfam
PF00860 Xan_ur_permease, 1 hit
TIGRFAMsiTIGR00801 ncs2, 1 hit
TIGR03173 pbuX, 1 hit
PROSITEiView protein in PROSITE
PS01116 XANTH_URACIL_PERMASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q46821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIDSQLPS SSGQDRPTDE VDRILSPGKL IILGLQHVLV MYAGAVAVPL
60 70 80 90 100
MIGDRLGLSK EAIAMLISSD LFCCGIVTLL QCIGIGRFMG IRLPVIMSVT
110 120 130 140 150
FAAVTPMIAI GMNPDIGLLG IFGATIAAGF ITTLLAPLIG RLMPLFPPLV
160 170 180 190 200
TGVVITSIGL SIIQVGIDWA AGGKGNPQYG NPVYLGISFA VLIFILLITR
210 220 230 240 250
YAKGFMSNVA VLLGIVFGFL LSWMMNEVNL SGLHDASWFA IVTPMSFGMP
260 270 280 290 300
IFDPVSILTM TAVLIIVFIE SMGMFLALGE IVGRKLSSHD IIRGLRVDGV
310 320 330 340 350
GTMIGGTFNS FPHTSFSQNV GLVSVTRVHS RWVCISSGII LILFGMVPKM
360 370 380 390 400
AVLVASIPQF VLGGAGLVMF GMVLATGIRI LSRCNYTTNR YNLYIVAISL
410 420 430 440 450
GVGMTPTLSH DFFSKLPAVL QPLLHSGIML ATLSAVVLNV FFNGYQHHAD
460 470 480
LVKESVSDKD LKVRTVRMWL LMRKLKKNEH GE
Length:482
Mass (Da):51,758
Last modified:May 30, 2000 - v2
Checksum:iE08EF1D4CBD9D066
GO

Sequence cautioni

The sequence AAA83069 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA Translation: AAA83069.1 Different initiation.
U00096 Genomic DNA Translation: AAC75926.2
AP009048 Genomic DNA Translation: BAE76953.1
PIRiH65072
RefSeqiNP_417364.2, NC_000913.3
WP_001295374.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75926; AAC75926; b2888
BAE76953; BAE76953; BAE76953
GeneIDi949017
KEGGiecj:JW5470
eco:b2888
PATRICifig|1411691.4.peg.3847

Similar proteinsi

Entry informationi

Entry nameiUACT_ECOLI
AccessioniPrimary (citable) accession number: Q46821
Secondary accession number(s): Q2M9V3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: April 25, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health