Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Uric acid transporter UacT

Gene

uacT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-dependent high-capacity transporter for uric acid. Shows also a low capacity for transport of xanthine at 37 degrees Celsius but not at 25 degrees Celsius.1 Publication

Enzyme regulationi

Inhibited in the presence of the protonophore carbonyl cyanide m-chlorophenyl hydrazone.1 Publication

Kineticsi

  1. KM=0.5 mM for uric acid1 Publication
  2. KM=0.3 mM for xanthine1 Publication
  1. Vmax=715 nmol/min/mg enzyme with acid uric as substrate1 Publication
  2. Vmax=14 nmol/min/mg enzyme with xanthine as substrate (at 37 degrees Celsius)1 Publication

GO - Molecular functioni

  • symporter activity Source: UniProtKB-KW
  • urate transmembrane transporter activity Source: EcoCyc
  • xanthine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • organic acid transmembrane transport Source: GOC
  • proton transport Source: UniProtKB-KW
  • urate transport Source: EcoCyc
  • xanthine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YGFU-MONOMER.
ECOL316407:JW5470-MONOMER.
MetaCyc:YGFU-MONOMER.

Protein family/group databases

TCDBi2.A.40.3.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Uric acid transporter UacT
Gene namesi
Name:uacT
Synonyms:ygfU
Ordered Locus Names:b2888, JW5470
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13071. uacT.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929CytoplasmicSequence analysisAdd
BLAST
Transmembranei30 – 5021HelicalSequence analysisAdd
BLAST
Topological domaini51 – 6212PeriplasmicSequence analysisAdd
BLAST
Transmembranei63 – 8321HelicalSequence analysisAdd
BLAST
Topological domaini84 – 929CytoplasmicSequence analysis
Transmembranei93 – 11321HelicalSequence analysisAdd
BLAST
Topological domaini114 – 1152PeriplasmicSequence analysis
Transmembranei116 – 13621HelicalSequence analysisAdd
BLAST
Topological domaini137 – 1426CytoplasmicSequence analysis
Transmembranei143 – 16321HelicalSequence analysisAdd
BLAST
Topological domaini164 – 17815PeriplasmicSequence analysisAdd
BLAST
Transmembranei179 – 19921HelicalSequence analysisAdd
BLAST
Topological domaini200 – 2045CytoplasmicSequence analysis
Transmembranei205 – 22521HelicalSequence analysisAdd
BLAST
Topological domaini226 – 26136PeriplasmicSequence analysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Topological domaini283 – 33755CytoplasmicSequence analysisAdd
BLAST
Transmembranei338 – 35821HelicalSequence analysisAdd
BLAST
Topological domaini359 – 3591PeriplasmicSequence analysis
Transmembranei360 – 38021HelicalSequence analysisAdd
BLAST
Topological domaini381 – 39212CytoplasmicSequence analysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence analysisAdd
BLAST
Topological domaini414 – 4218PeriplasmicSequence analysis
Transmembranei422 – 44221HelicalSequence analysisAdd
BLAST
Topological domaini443 – 48240CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi37 – 371H → K or L: Lack of activity. 1 Publication
Mutagenesisi37 – 371H → N: Strong decrease in activity. 1 Publication
Mutagenesisi100 – 1001T → A: Decrease in activity with uric acid. Highly active with xanthine. 1 Publication
Mutagenesisi100 – 1001T → S: Decrease in activity. 1 Publication
Mutagenesisi256 – 2561S → Q: Almost no change in activity. 1 Publication
Mutagenesisi259 – 2591T → V: Almost no change in activity. 1 Publication
Mutagenesisi270 – 2701E → D, N or Q: Lack of activity. 1 Publication
Mutagenesisi278 – 2781L → T: Almost no change in activity. 1 Publication
Mutagenesisi298 – 2981D → E: Strong decrease in activity. 1 Publication
Mutagenesisi298 – 2981D → N: Lack of activity. 1 Publication
Mutagenesisi317 – 3171S → A: Decrease in activity. 1 Publication
Mutagenesisi318 – 3181Q → E or N: Strong decrease in activity. 1 Publication
Mutagenesisi319 – 3191N → D or Q: Lack of activity. 1 Publication
Mutagenesisi320 – 3201V → N: Lack of activity. 1 Publication
Mutagenesisi327 – 3271R → G: Almost no change in activity. 1 Publication
Mutagenesisi426 – 4261S → N: Almost no change in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Uric acid transporter UacTPRO_0000165968Add
BLAST

Proteomic databases

PaxDbiQ46821.

Interactioni

Protein-protein interaction databases

BioGridi4262336. 160 interactions.
DIPiDIP-12176N.
STRINGi511145.b2888.

Structurei

3D structure databases

ProteinModelPortaliQ46821.
SMRiQ46821. Positions 29-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038199.
InParanoidiQ46821.
OMAiFYFGVPE.
OrthoDBiEOG6M6JJK.
PhylomeDBiQ46821.

Family and domain databases

InterProiIPR017588. UacT-like.
IPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
TIGR03173. pbuX. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIDSQLPS SSGQDRPTDE VDRILSPGKL IILGLQHVLV MYAGAVAVPL
60 70 80 90 100
MIGDRLGLSK EAIAMLISSD LFCCGIVTLL QCIGIGRFMG IRLPVIMSVT
110 120 130 140 150
FAAVTPMIAI GMNPDIGLLG IFGATIAAGF ITTLLAPLIG RLMPLFPPLV
160 170 180 190 200
TGVVITSIGL SIIQVGIDWA AGGKGNPQYG NPVYLGISFA VLIFILLITR
210 220 230 240 250
YAKGFMSNVA VLLGIVFGFL LSWMMNEVNL SGLHDASWFA IVTPMSFGMP
260 270 280 290 300
IFDPVSILTM TAVLIIVFIE SMGMFLALGE IVGRKLSSHD IIRGLRVDGV
310 320 330 340 350
GTMIGGTFNS FPHTSFSQNV GLVSVTRVHS RWVCISSGII LILFGMVPKM
360 370 380 390 400
AVLVASIPQF VLGGAGLVMF GMVLATGIRI LSRCNYTTNR YNLYIVAISL
410 420 430 440 450
GVGMTPTLSH DFFSKLPAVL QPLLHSGIML ATLSAVVLNV FFNGYQHHAD
460 470 480
LVKESVSDKD LKVRTVRMWL LMRKLKKNEH GE
Length:482
Mass (Da):51,758
Last modified:May 30, 2000 - v2
Checksum:iE08EF1D4CBD9D066
GO

Sequence cautioni

The sequence AAA83069.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83069.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75926.2.
AP009048 Genomic DNA. Translation: BAE76953.1.
PIRiH65072.
RefSeqiNP_417364.2. NC_000913.3.
WP_001295374.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75926; AAC75926; b2888.
BAE76953; BAE76953; BAE76953.
GeneIDi949017.
KEGGiecj:JW5470.
eco:b2888.
PATRICi32121184. VBIEscCol129921_2980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83069.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75926.2.
AP009048 Genomic DNA. Translation: BAE76953.1.
PIRiH65072.
RefSeqiNP_417364.2. NC_000913.3.
WP_001295374.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliQ46821.
SMRiQ46821. Positions 29-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262336. 160 interactions.
DIPiDIP-12176N.
STRINGi511145.b2888.

Protein family/group databases

TCDBi2.A.40.3.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiQ46821.

Protocols and materials databases

DNASUi949017.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75926; AAC75926; b2888.
BAE76953; BAE76953; BAE76953.
GeneIDi949017.
KEGGiecj:JW5470.
eco:b2888.
PATRICi32121184. VBIEscCol129921_2980.

Organism-specific databases

EchoBASEiEB2882.
EcoGeneiEG13071. uacT.

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038199.
InParanoidiQ46821.
OMAiFYFGVPE.
OrthoDBiEOG6M6JJK.
PhylomeDBiQ46821.

Enzyme and pathway databases

BioCyciEcoCyc:YGFU-MONOMER.
ECOL316407:JW5470-MONOMER.
MetaCyc:YGFU-MONOMER.

Miscellaneous databases

PROiQ46821.

Family and domain databases

InterProiIPR017588. UacT-like.
IPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
TIGR03173. pbuX. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Substrate selectivity of YgfU, a uric acid transporter from Escherichia coli."
    Papakostas K., Frillingos S.
    J. Biol. Chem. 287:15684-15695(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, GENE NAME, MUTAGENESIS OF HIS-37; THR-100; SER-256; THR-259; GLU-270; LEU-278; ASP-298; SER-317; GLN-318; ASN-319; VAL-320; ARG-327 AND SER-426.
    Strain: K12.

Entry informationi

Entry nameiUACT_ECOLI
AccessioniPrimary (citable) accession number: Q46821
Secondary accession number(s): Q2M9V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: January 20, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.