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Protein

Guanine/hypoxanthine permease GhxQ

Gene

ghxQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for guanine and hypoxanthine.1 Publication

Kineticsi

  1. KM=1.8 µM for guanine1 Publication
  2. KM=23.2 µM for hypoxanthine1 Publication
  1. Vmax=3.4 nmol/min/mg enzyme with guanine as substrate1 Publication
  2. Vmax=13.8 nmol/min/mg enzyme with hypoxanthine as substrate1 Publication

GO - Molecular functioni

  • guanine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • guanine import into cell Source: EcoCyc
  • guanine transmembrane transport Source: GOC
  • hypoxanthine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7504-MONOMER.
ECOL316407:JW5467-MONOMER.
MetaCyc:G7504-MONOMER.

Protein family/group databases

TCDBi2.A.40.7.7. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine/hypoxanthine permease GhxQ
Gene namesi
Name:ghxQ
Synonyms:ygfQ, ygfR
Ordered Locus Names:b4464, JW5467
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13067. ghxQ.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence analysisAdd
BLAST
Transmembranei32 – 5524HelicalSequence analysisAdd
BLAST
Topological domaini56 – 6510PeriplasmicSequence analysis
Transmembranei66 – 8419HelicalSequence analysisAdd
BLAST
Topological domaini85 – 862CytoplasmicSequence analysis
Transmembranei87 – 10317Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini104 – 11512PeriplasmicSequence analysisAdd
BLAST
Transmembranei116 – 13520HelicalSequence analysisAdd
BLAST
Topological domaini136 – 14712CytoplasmicSequence analysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence analysisAdd
BLAST
Topological domaini169 – 18618PeriplasmicSequence analysisAdd
BLAST
Transmembranei187 – 20418HelicalSequence analysisAdd
BLAST
Topological domaini205 – 2084CytoplasmicSequence analysis
Transmembranei209 – 22820HelicalSequence analysisAdd
BLAST
Topological domaini229 – 26032PeriplasmicSequence analysisAdd
BLAST
Transmembranei261 – 28929HelicalSequence analysisAdd
BLAST
Topological domaini290 – 30213CytoplasmicSequence analysisAdd
BLAST
Transmembranei303 – 31816HelicalSequence analysisAdd
BLAST
Topological domaini319 – 3202PeriplasmicSequence analysis
Transmembranei321 – 33616Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini337 – 3404CytoplasmicSequence analysis
Transmembranei341 – 35515HelicalSequence analysisAdd
BLAST
Topological domaini356 – 36611PeriplasmicSequence analysisAdd
BLAST
Transmembranei367 – 38620HelicalSequence analysisAdd
BLAST
Topological domaini387 – 3915CytoplasmicSequence analysis
Intramembranei392 – 42736Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini428 – 45528CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455Guanine/hypoxanthine permease GhxQPRO_0000169358Add
BLAST

Proteomic databases

PaxDbiQ46817.

Interactioni

Protein-protein interaction databases

BioGridi4259228. 164 interactions.
STRINGi511145.b4464.

Structurei

3D structure databases

ProteinModelPortaliQ46817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244363.
InParanoidiQ46817.
KOiK06901.
OMAiGFPHTAV.
OrthoDBiEOG6DNT97.
PhylomeDBiQ46817.

Family and domain databases

InterProiIPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q46817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDILQTPD APKPQGALDN YFKITARGST VRQEVLAGLT TFLAMVYSVI
60 70 80 90 100
VVPGMLGKAG FPPAAVFVAT CLVAGFGSLL MGLWANLPMA IGCAISLTAF
110 120 130 140 150
TAFSLVLGQQ ISVPVALGAV FLMGVIFTAI SVTGVRTWIL RNLPMGIAHG
160 170 180 190 200
TGIGIGLFLL LIAANGVGMV IKNPIEGLPV ALGAFTSFPV MMSLLGLAVI
210 220 230 240 250
FGLEKCRVPG GILLVIIAIS IIGLIFDPAV KYHGLVAMPS LTGEDGKSLI
260 270 280 290 300
FSLDIMGALQ PTVLPSVLAL VMTAVFDATG TIRAVAGQAN LLDKDNQIIN
310 320 330 340 350
GGKALTSDSV SSIFSGLVGA APAAVYIESA AGTAAGGKTG LTATVVGALF
360 370 380 390 400
LLILFLSPLS FLIPGYATAP ALMYVGLLML SNVSKLDFND FIDAMAGLVC
410 420 430 440 450
AVFIVLTCNI VTGIMLGFVT LVVGRVFARE WQKLNIGTVI ITAALVAFYA

GGWAI
Length:455
Mass (Da):46,758
Last modified:November 23, 2004 - v2
Checksum:iB56EDA4AAA01C797
GO

Sequence cautioni

The sequence AAA83065.1 differs from that shown. Reason: Frameshift at position 179. Produces two separate ORFs (ygfQ and ygfR).Curated
The sequence AAA83066.1 differs from that shown. Reason: Frameshift at position 179. Produces two separate ORFs (ygfQ and ygfR).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83065.1. Frameshift.
U28375 Genomic DNA. Translation: AAA83066.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48153.1.
AP009048 Genomic DNA. Translation: BAE76950.1.
PIRiD65072.
E65072.
RefSeqiWP_000012163.1. NZ_LN832404.1.
YP_026186.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48153; AAT48153; b4464.
BAE76950; BAE76950; BAE76950.
GeneIDi2847748.
KEGGiecj:JW5467.
eco:b4464.
PATRICi32121178. VBIEscCol129921_2977.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83065.1. Frameshift.
U28375 Genomic DNA. Translation: AAA83066.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48153.1.
AP009048 Genomic DNA. Translation: BAE76950.1.
PIRiD65072.
E65072.
RefSeqiWP_000012163.1. NZ_LN832404.1.
YP_026186.1. NC_000913.3.

3D structure databases

ProteinModelPortaliQ46817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259228. 164 interactions.
STRINGi511145.b4464.

Protein family/group databases

TCDBi2.A.40.7.7. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiQ46817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48153; AAT48153; b4464.
BAE76950; BAE76950; BAE76950.
GeneIDi2847748.
KEGGiecj:JW5467.
eco:b4464.
PATRICi32121178. VBIEscCol129921_2977.

Organism-specific databases

EchoBASEiEB2879.
EcoGeneiEG13067. ghxQ.

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244363.
InParanoidiQ46817.
KOiK06901.
OMAiGFPHTAV.
OrthoDBiEOG6DNT97.
PhylomeDBiQ46817.

Enzyme and pathway databases

BioCyciEcoCyc:G7504-MONOMER.
ECOL316407:JW5467-MONOMER.
MetaCyc:G7504-MONOMER.

Miscellaneous databases

PROiQ46817.

Family and domain databases

InterProiIPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: SEQUENCE REVISION.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Functional identification of the hypoxanthine/guanine transporters YjcD and YgfQ and the adenine transporters PurP and YicO of Escherichia coli K-12."
    Papakostas K., Botou M., Frillingos S.
    J. Biol. Chem. 288:36827-36840(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, GENE NAME.
    Strain: K12.

Entry informationi

Entry nameiGHXQ_ECOLI
AccessioniPrimary (citable) accession number: Q46817
Secondary accession number(s): Q2M9V6, Q46818, Q6BF61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 23, 2004
Last modified: January 20, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.