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UniProtKB - Q46817 (GHXQ_ECOLI)

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Protein

Guanine/hypoxanthine permease GhxQ

Gene

ghxQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for guanine and hypoxanthine.1 Publication

Kineticsi

  1. KM=1.8 µM for guanine1 Publication
  2. KM=23.2 µM for hypoxanthine1 Publication
  1. Vmax=3.4 nmol/min/mg enzyme with guanine as substrate1 Publication
  2. Vmax=13.8 nmol/min/mg enzyme with hypoxanthine as substrate1 Publication

GO - Molecular functioni

  • guanine transmembrane transporter activity Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  • hypoxanthine transport Source: EcoCyc
Complete GO annotation...

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:G7504-MONOMER.
MetaCyc:G7504-MONOMER.

Protein family/group databases

TCDBi2.A.40.7.7. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine/hypoxanthine permease GhxQ
Gene namesi
Name:ghxQ
Synonyms:ygfQ, ygfR
Ordered Locus Names:b4464, JW5467
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13067. ghxQ.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31CytoplasmicSequence analysisAdd BLAST31
Transmembranei32 – 55HelicalSequence analysisAdd BLAST24
Topological domaini56 – 65PeriplasmicSequence analysis10
Transmembranei66 – 84HelicalSequence analysisAdd BLAST19
Topological domaini85 – 86CytoplasmicSequence analysis2
Transmembranei87 – 103Discontinuously helicalSequence analysisAdd BLAST17
Topological domaini104 – 115PeriplasmicSequence analysisAdd BLAST12
Transmembranei116 – 135HelicalSequence analysisAdd BLAST20
Topological domaini136 – 147CytoplasmicSequence analysisAdd BLAST12
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 186PeriplasmicSequence analysisAdd BLAST18
Transmembranei187 – 204HelicalSequence analysisAdd BLAST18
Topological domaini205 – 208CytoplasmicSequence analysis4
Transmembranei209 – 228HelicalSequence analysisAdd BLAST20
Topological domaini229 – 260PeriplasmicSequence analysisAdd BLAST32
Transmembranei261 – 289HelicalSequence analysisAdd BLAST29
Topological domaini290 – 302CytoplasmicSequence analysisAdd BLAST13
Transmembranei303 – 318HelicalSequence analysisAdd BLAST16
Topological domaini319 – 320PeriplasmicSequence analysis2
Transmembranei321 – 336Discontinuously helicalSequence analysisAdd BLAST16
Topological domaini337 – 340CytoplasmicSequence analysis4
Transmembranei341 – 355HelicalSequence analysisAdd BLAST15
Topological domaini356 – 366PeriplasmicSequence analysisAdd BLAST11
Transmembranei367 – 386HelicalSequence analysisAdd BLAST20
Topological domaini387 – 391CytoplasmicSequence analysis5
Intramembranei392 – 427Discontinuously helicalSequence analysisAdd BLAST36
Topological domaini428 – 455CytoplasmicSequence analysisAdd BLAST28

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001693581 – 455Guanine/hypoxanthine permease GhxQAdd BLAST455

Proteomic databases

PaxDbiQ46817.
PRIDEiQ46817.

Interactioni

Protein-protein interaction databases

BioGridi4259228. 164 interactors.
STRINGi511145.b4464.

Structurei

3D structure databases

ProteinModelPortaliQ46817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244363.
InParanoidiQ46817.
KOiK06901.
PhylomeDBiQ46817.

Family and domain databases

InterProiView protein in InterPro
IPR006043. Xant/urac/vitC.
PfamiView protein in Pfam
PF00860. Xan_ur_permease. 1 hit.

Sequencei

Sequence statusi: Complete.

Q46817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDILQTPD APKPQGALDN YFKITARGST VRQEVLAGLT TFLAMVYSVI 
        60         70         80         90        100
VVPGMLGKAG FPPAAVFVAT CLVAGFGSLL MGLWANLPMA IGCAISLTAF 
       110        120        130        140        150
TAFSLVLGQQ ISVPVALGAV FLMGVIFTAI SVTGVRTWIL RNLPMGIAHG 
       160        170        180        190        200
TGIGIGLFLL LIAANGVGMV IKNPIEGLPV ALGAFTSFPV MMSLLGLAVI 
       210        220        230        240        250
FGLEKCRVPG GILLVIIAIS IIGLIFDPAV KYHGLVAMPS LTGEDGKSLI 
       260        270        280        290        300
FSLDIMGALQ PTVLPSVLAL VMTAVFDATG TIRAVAGQAN LLDKDNQIIN 
       310        320        330        340        350
GGKALTSDSV SSIFSGLVGA APAAVYIESA AGTAAGGKTG LTATVVGALF 
       360        370        380        390        400
LLILFLSPLS FLIPGYATAP ALMYVGLLML SNVSKLDFND FIDAMAGLVC 
       410        420        430        440        450
AVFIVLTCNI VTGIMLGFVT LVVGRVFARE WQKLNIGTVI ITAALVAFYA 

GGWAI
Length:455
Mass (Da):46,758
Last modified:November 23, 2004 - v2
Checksum:iB56EDA4AAA01C797
GO

Sequence cautioni

The sequence AAA83065 differs from that shown. Reason: Frameshift at position 179. Produces two separate ORFs (ygfQ and ygfR).Curated
The sequence AAA83066 differs from that shown. Reason: Frameshift at position 179. Produces two separate ORFs (ygfQ and ygfR).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83065.1. Frameshift.
U28375 Genomic DNA. Translation: AAA83066.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48153.1.
AP009048 Genomic DNA. Translation: BAE76950.1.
PIRiD65072.
E65072.
RefSeqiWP_000012163.1. NZ_LN832404.1.
YP_026186.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48153; AAT48153; b4464.
BAE76950; BAE76950; BAE76950.
GeneIDi2847748.
KEGGiecj:JW5467.
eco:b4464.
PATRICifig|1411691.4.peg.3850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83065.1. Frameshift.
U28375 Genomic DNA. Translation: AAA83066.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48153.1.
AP009048 Genomic DNA. Translation: BAE76950.1.
PIRiD65072.
E65072.
RefSeqiWP_000012163.1. NZ_LN832404.1.
YP_026186.1. NC_000913.3.

3D structure databases

ProteinModelPortaliQ46817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259228. 164 interactors.
STRINGi511145.b4464.

Protein family/group databases

TCDBi2.A.40.7.7. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiQ46817.
PRIDEiQ46817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48153; AAT48153; b4464.
BAE76950; BAE76950; BAE76950.
GeneIDi2847748.
KEGGiecj:JW5467.
eco:b4464.
PATRICifig|1411691.4.peg.3850.

Organism-specific databases

EchoBASEiEB2879.
EcoGeneiEG13067. ghxQ.

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244363.
InParanoidiQ46817.
KOiK06901.
PhylomeDBiQ46817.

Enzyme and pathway databases

BioCyciEcoCyc:G7504-MONOMER.
MetaCyc:G7504-MONOMER.

Miscellaneous databases

PROiPR:Q46817.

Family and domain databases

InterProiView protein in InterPro
IPR006043. Xant/urac/vitC.
PfamiView protein in Pfam
PF00860. Xan_ur_permease. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGHXQ_ECOLI
AccessioniPrimary (citable) accession number: Q46817
Secondary accession number(s): Q2M9V6, Q46818, Q6BF61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 23, 2004
Last modified: June 7, 2017
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.