Q46814 (XDHD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable hypoxanthine oxidase XdhD
EC=1.-.-.-

Gene names

Name:	xdhD
Synonyms:	ygfN
Ordered Locus Names:	b2881, JW2849

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	956 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probably has no xanthine dehydrogenase activity; however deletion results in increased adenine sensitivity, suggesting that this protein contributes to the conversion of adenine to guanine nucleotides during purine salvage. Ref.3
Cofactor	Binds 2 2Fe-2S centers Potential. Binds 1 molybdenum ion (molybdopterin) per subunit By similarity.
Sequence similarities	Belongs to the xanthine dehydrogenase family.

Ontologies

Keywords
Biological process	Purine metabolism Purine salvage
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from direct assay PubMed 22094925. Source: EcoCyc oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 956	956	Probable hypoxanthine oxidase XdhD		PRO_0000166096
Sites
	Metal binding	414	1	Molybdenum Potential
	Metal binding	445	1	Molybdenum; via carbonyl oxygen Potential
	Metal binding	727	1	Molybdenum; via amide nitrogen Potential

Sequences

	Sequence		Length	Mass (Da)
	Q46814 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F60EEFE06F0AC17D	FASTA	956	103,519
	10 20 30 40 50 60 MIIHFTLNGA PQELTVNPGE NVQKLLFNMG MHSVRNSDDG FGFAGSDAII FNGNIVNASL 70 80 90 100 110 120 LIAAQLEKAD IRTAESLGKW NELSLVQQAM VDVGVVQSGY NDPAAALIIT DLLDRIAAPT 130 140 150 160 170 180 REEIDDALSG LFSRDAGWQQ YYQVIELAVA RKNNPQATID IAPTFRDDLE VIGKHYPKTD 190 200 210 220 230 240 AAKMVQAKPC YVEDRVTADA CVIKMLRSPH AHALITHLDV SKAEALPGVV HVITHLNCPD 250 260 270 280 290 300 IYYTPGGQSA PEPSPLDRRM FGKKMRHVGD RVAAVVAESE EIALEALKLI DVEYEVLKPV 310 320 330 340 350 360 MSIDEAMAED APVVHDEPVV YVAGAPDTLE DDNSHAAQRG EHMIINFPIG SRPRKNIAAS 370 380 390 400 410 420 IHGHIGDMDK GFADADVIIE RTYNSTQAQQ CPTETHICFT RMDGDRLVIH ASTQVPWHLR 430 440 450 460 470 480 RQVARLVGMK QHKVHVIKER VGGGFGSKQD ILLEEVCAWA TCVTGRPVLF RYTREEEFIA 490 500 510 520 530 540 NTSRHVAKVT VKLGAKKDGR LTAVKMDFRA NTGPYGNHSL TVPCNGPALS LPLYPCDNVD 550 560 570 580 590 600 FQVTTYYSNI CPNGAYQGYG APKGNFAITM ALAELAEQLQ IDQLEIIERN RVHEGQELKI 610 620 630 640 650 660 LGAIGEGKAP TSVPSAASCA LEEILRQGRE MIQWSSPKPQ NGDWHIGRGV AIIMQKSGIP 670 680 690 700 710 720 DIDQANCMIK LESDGTFIVH SGGADIGTGL DTVVTKLAAE VLHCPPQDVH VISGDTDHAL 730 740 750 760 770 780 FDKGAYASSG TCFSGNAARL AAENLREKIL FHGAQMLGEP VADVQLATPG VVRGKKGEVS 790 800 810 820 830 840 FGDIAHKGET GTGFGSLVGT GSYITPDFAF PYGANFAEVA VNTRTGEIRL DKFYALLDCG 850 860 870 880 890 900 TPVNPELALG QIYGATLRAI GHSMSEEIIY DAEGHPLTRD LRSYGAPKIG DIPRDFRAVL 910 920 930 940 950 VPSDDKVGPF GAKSISEIGV NGAAPAIATA IHDACGIWLR EWHFTPEKIL TALEKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage." Xi H., Schneider B.L., Reitzer L. J. Bacteriol. 182:5332-5341(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28375 Genomic DNA. Translation: AAA83062.1. U00096 Genomic DNA. Translation: AAC75919.1. AP009048 Genomic DNA. Translation: BAE76947.1.
PIR	A65072.
RefSeq	NP_417357.1. NC_000913.2. YP_491083.1. NC_007779.1.
3D structure databases
ProteinModelPortal	Q46814.
SMR	Q46814. Positions 1-954.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-28056N.
IntAct	Q46814. 3 interactions.
STRING	511145.b2881.
Proteomic databases
PRIDE	Q46814.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75919; AAC75919; b2881. BAE76947; BAE76947; BAE76947.
GeneID	12933334. 949079.
KEGG	ecj:Y75_p2814. eco:b2881.
PATRIC	32121172. VBIEscCol129921_2974.
Organism-specific databases
EchoBASE	EB2876.
EcoGene	EG13064. xdhD.
Phylogenomic databases
eggNOG	COG1529.
HOGENOM	HOG000244715.
KO	K12528.
OMA	EIATRNM.
ProtClustDB	PRK09800.
Enzyme and pathway databases
BioCyc	EcoCyc:G7500-MONOMER. ECOL316407:JW2849-MONOMER. MetaCyc:G7500-MONOMER.
Gene expression databases
Genevestigator	Q46814.
Family and domain databases
Gene3D	1.10.150.120. 1 hit. 3.30.365.10. 7 hits. 3.90.1170.50. 1 hit.
InterPro	IPR002888. 2Fe-2S-bd. IPR001041. 2Fe-2S_ferredoxin-type. IPR000674. Ald_Oxase/Xan_DH_a/b. IPR008274. AldOxase/xan_DH_Mopterin-bd. IPR017699. Se_red_Mo-bd_su. [Graphical view]
Pfam	PF01315. Ald_Xan_dh_C. 1 hit. PF02738. Ald_Xan_dh_C2. 1 hit. PF01799. Fer2_2. 1 hit. [Graphical view]
SMART	SM01008. Ald_Xan_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF47741. 2Fe-2S_bind. 1 hit. SSF56003. Ald_xan_DH_mo_bd. 1 hit. SSF54665. Aldxan_dh_hamm. 1 hit. SSF54292. Ferredoxin. 1 hit.
TIGRFAMs	TIGR03313. Se_sel_red_Mo. 1 hit.
ProtoNet	Search...

Entry information

Entry name

XDHD_ECOLI

Accession

Primary (citable) accession number: Q46814
Secondary accession number(s): Q2M9V9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program